{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q10Q08","secondaryAccessions":["A0A0P0VUM6","Q7Y069"],"uniProtkbId":"RFA1B_ORYSJ","entryAudit":{"firstPublicDate":"2013-06-26","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2006-08-22","entryVersion":123,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Oryza sativa subsp. japonica","commonName":"Rice","taxonId":39947,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","Liliopsida","Poales","Poaceae","BOP clade","Oryzoideae","Oryzeae","Oryzinae","Oryza","Oryza sativa"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Replication protein A 70 kDa DNA-binding subunit B"},"shortNames":[{"value":"OsRPA70b"}]},"alternativeNames":[{"fullName":{"value":"Replication factor A protein 1B"}},{"fullName":{"value":"Replication protein A 1B"}}]},"genes":[{"geneName":{"value":"RPA1B"},"synonyms":[{"value":"RPA70B"}],"orderedLocusNames":[{"value":"Os03g0214100"},{"value":"LOC_Os03g11540"}],"orfNames":[{"value":"OsJ_09906"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15978034"}],"value":"Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15978034"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16428324"}],"value":"Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex) (By similarity). Interacts with RPA2A"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11470540"}],"value":"Expressed in root tips, roots, shoot apical meristem (SAM) and young leaves, and at lower levels in mature leaves, flag leaves and ears"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11470540"}],"value":"Repressed by sucrose starvation"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the replication factor A protein 1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":630,"modifier":"EXACT"}},"description":"Replication protein A 70 kDa DNA-binding subunit B","featureId":"PRO_0000422621"},{"type":"DNA binding","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":282,"modifier":"EXACT"}},"description":"OB"},{"type":"Zinc finger","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":516,"modifier":"EXACT"}},"description":"C4-type","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":269,"modifier":"EXACT"},"end":{"value":269,"modifier":"EXACT"}},"description":"in Ref. 1; BAC77529","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":411,"modifier":"EXACT"}},"description":"in Ref. 1; BAC77529","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["L"]}}],"keywords":[{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0233","category":"Biological process","name":"DNA recombination"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0235","category":"Biological process","name":"DNA replication"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0862","category":"Ligand","name":"Zinc"},{"id":"KW-0863","category":"Domain","name":"Zinc-finger"}],"references":[{"referenceNumber":1,"citation":{"id":"11470540","citationType":"journal article","authors":["Ishibashi T.","Kimura S.","Furukawa T.","Hatanaka M.","Hashimoto J.","Sakaguchi K."],"citationCrossReferences":[{"database":"PubMed","id":"11470540"},{"database":"DOI","id":"10.1016/s0378-1119(01)00555-8"}],"title":"Two types of replication protein A 70 kDa subunit in rice, Oryza sativa: molecular cloning, characterization, and cellular & tissue distribution.","publicationDate":"2001","journal":"Gene","firstPage":"335","lastPage":"343","volume":"272"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","TISSUE SPECIFICITY","INDUCTION"],"referenceComments":[{"value":"cv. Nipponbare","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16109971","citationType":"journal article","authoringGroup":["The rice chromosome 3 sequencing consortium"],"authors":["Buell C.R.","Yuan Q.","Ouyang S.","Liu J.","Zhu W.","Wang A.","Maiti R.","Haas B.","Wortman J.","Pertea M.","Jones K.M.","Kim M.","Overton L.","Tsitrin T.","Fadrosh D.","Bera J.","Weaver B.","Jin S.","Johri S.","Reardon M.","Webb K.","Hill J.","Moffat K.","Tallon L.","Van Aken S.","Lewis M.","Utterback T.","Feldblyum T.","Zismann V.","Iobst S.","Hsiao J.","de Vazeille A.R.","Salzberg S.L.","White O.","Fraser C.M.","Yu Y.","Kim H.","Rambo T.","Currie J.","Collura K.","Kernodle-Thompson S.","Wei F.","Kudrna K.","Ammiraju J.S.S.","Luo M.","Goicoechea J.L.","Wing R.A.","Henry D.","Oates R.","Palmer M.","Pries G.","Saski C.","Simmons J.","Soderlund C.","Nelson W.","de la Bastide M.","Spiegel L.","Nascimento L.","Huang E.","Preston R.","Zutavern T.","Palmer L.","O'Shaughnessy A.","Dike S.","McCombie W.R.","Minx P.","Cordum H.","Wilson R.","Jin W.","Lee H.R.","Jiang J.","Jackson S."],"citationCrossReferences":[{"database":"PubMed","id":"16109971"},{"database":"DOI","id":"10.1101/gr.3869505"}],"title":"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species.","publicationDate":"2005","journal":"Genome Res.","firstPage":"1284","lastPage":"1291","volume":"15"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Nipponbare","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16100779","citationType":"journal article","authoringGroup":["International rice genome sequencing project (IRGSP)"],"citationCrossReferences":[{"database":"PubMed","id":"16100779"},{"database":"DOI","id":"10.1038/nature03895"}],"title":"The map-based sequence of the rice genome.","publicationDate":"2005","journal":"Nature","firstPage":"793","lastPage":"800","volume":"436"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Nipponbare","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"18089549","citationType":"journal article","authoringGroup":["The rice annotation project (RAP)"],"citationCrossReferences":[{"database":"PubMed","id":"18089549"},{"database":"DOI","id":"10.1093/nar/gkm978"}],"title":"The rice annotation project database (RAP-DB): 2008 update.","publicationDate":"2008","journal":"Nucleic Acids Res.","firstPage":"D1028","lastPage":"D1033","volume":"36"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Nipponbare","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"24280374","citationType":"journal article","authors":["Kawahara Y.","de la Bastide M.","Hamilton J.P.","Kanamori H.","McCombie W.R.","Ouyang S.","Schwartz D.C.","Tanaka T.","Wu J.","Zhou S.","Childs K.L.","Davidson R.M.","Lin H.","Quesada-Ocampo L.","Vaillancourt B.","Sakai H.","Lee S.S.","Kim J.","Numa H.","Itoh T.","Buell C.R.","Matsumoto T."],"citationCrossReferences":[{"database":"PubMed","id":"24280374"},{"database":"DOI","id":"10.1186/1939-8433-6-4"}],"title":"Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data.","publicationDate":"2013","journal":"Rice","firstPage":"4","lastPage":"4","volume":"6"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Nipponbare","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"15685292","citationType":"journal article","authors":["Yu J.","Wang J.","Lin W.","Li S.","Li H.","Zhou J.","Ni P.","Dong W.","Hu S.","Zeng C.","Zhang J.","Zhang Y.","Li R.","Xu Z.","Li S.","Li X.","Zheng H.","Cong L.","Lin L.","Yin J.","Geng J.","Li G.","Shi J.","Liu J.","Lv H.","Li J.","Wang J.","Deng Y.","Ran L.","Shi X.","Wang X.","Wu Q.","Li C.","Ren X.","Wang J.","Wang X.","Li D.","Liu D.","Zhang X.","Ji Z.","Zhao W.","Sun Y.","Zhang Z.","Bao J.","Han Y.","Dong L.","Ji J.","Chen P.","Wu S.","Liu J.","Xiao Y.","Bu D.","Tan J.","Yang L.","Ye C.","Zhang J.","Xu J.","Zhou Y.","Yu Y.","Zhang B.","Zhuang S.","Wei H.","Liu B.","Lei M.","Yu H.","Li Y.","Xu H.","Wei S.","He X.","Fang L.","Zhang Z.","Zhang Y.","Huang X.","Su Z.","Tong W.","Li J.","Tong Z.","Li S.","Ye J.","Wang L.","Fang L.","Lei T.","Chen C.-S.","Chen H.-C.","Xu Z.","Li H.","Huang H.","Zhang F.","Xu H.","Li N.","Zhao C.","Li S.","Dong L.","Huang Y.","Li L.","Xi Y.","Qi Q.","Li W.","Zhang B.","Hu W.","Zhang Y.","Tian X.","Jiao Y.","Liang X.","Jin J.","Gao L.","Zheng W.","Hao B.","Liu S.-M.","Wang W.","Yuan L.","Cao M.","McDermott J.","Samudrala R.","Wang J.","Wong G.K.-S.","Yang H."],"citationCrossReferences":[{"database":"PubMed","id":"15685292"},{"database":"DOI","id":"10.1371/journal.pbio.0030038"}],"title":"The genomes of Oryza sativa: a history of duplications.","publicationDate":"2005","journal":"PLoS Biol.","firstPage":"266","lastPage":"281","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Nipponbare","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"15978034","citationType":"journal article","authors":["Ishibashi T.","Koga A.","Yamamoto T.","Uchiyama Y.","Mori Y.","Hashimoto J.","Kimura S.","Sakaguchi K."],"citationCrossReferences":[{"database":"PubMed","id":"15978034"},{"database":"DOI","id":"10.1111/j.1742-4658.2005.04719.x"}],"title":"Two types of replication protein A in seed plants.","publicationDate":"2005","journal":"FEBS J.","firstPage":"3270","lastPage":"3281","volume":"272"},"referencePositions":["FUNCTION","INTERACTION WITH RPA2A"]},{"referenceNumber":8,"citation":{"id":"16428324","citationType":"journal article","authors":["Ishibashi T.","Kimura S.","Sakaguchi K."],"citationCrossReferences":[{"database":"PubMed","id":"16428324"},{"database":"DOI","id":"10.1093/jb/mvj014"}],"title":"A higher plant has three different types of RPA heterotrimeric complex.","publicationDate":"2006","journal":"J. Biochem.","firstPage":"99","lastPage":"104","volume":"139"},"referencePositions":["INTERACTION WITH RPA2A"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB111916","properties":[{"key":"ProteinId","value":"BAC77529.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"DP000009","properties":[{"key":"ProteinId","value":"ABF94627.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP008209","properties":[{"key":"ProteinId","value":"BAF11283.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP014959","properties":[{"key":"ProteinId","value":"BAS82952.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CM000140","properties":[{"key":"ProteinId","value":"EAZ26052.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q10Q08","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q10Q08","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q10Q08","properties":[{"key":"Number of interactors","value":"91"}]},{"database":"STRING","id":"39947.Q10Q08","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"39947-Q10Q08","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblPlants","id":"Os03t0214100-01","properties":[{"key":"ProteinId","value":"Os03t0214100-01"},{"key":"GeneId","value":"Os03g0214100"}]},{"database":"Gramene","id":"Os03t0214100-01","properties":[{"key":"ProteinId","value":"Os03t0214100-01"},{"key":"GeneId","value":"Os03g0214100"}]},{"database":"KEGG","id":"dosa:Os03g0214100","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0851","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_012393_3_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q10Q08","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FNSYAML","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"1751331at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PlantReactome","id":"R-OSA-9645850","properties":[{"key":"pathwayName","value":"Activation of pre-replication complex"}]},{"database":"Proteomes","id":"UP000059680","properties":[{"key":"Component","value":"Chromosome 3"}]},{"database":"GO","id":"GO:0005662","properties":[{"key":"GoTerm","value":"C:DNA replication factor A complex"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15978034"}]},{"database":"GO","id":"GO:0003684","properties":[{"key":"GoTerm","value":"F:damaged DNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0043047","properties":[{"key":"GoTerm","value":"F:single-stranded telomeric DNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006260","properties":[{"key":"GoTerm","value":"P:DNA replication"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000724","properties":[{"key":"GoTerm","value":"P:double-strand break repair via homologous recombination"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006289","properties":[{"key":"GoTerm","value":"P:nucleotide-excision repair"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0007004","properties":[{"key":"GoTerm","value":"P:telomere maintenance via telomerase"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd04474","properties":[{"key":"EntryName","value":"RPA1_DBD_A"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04475","properties":[{"key":"EntryName","value":"RPA1_DBD_B"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04476","properties":[{"key":"EntryName","value":"RPA1_DBD_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04477","properties":[{"key":"EntryName","value":"RPA1N"},{"key":"MatchStatus","value":"1"}]},{"database":"DisProt","id":"DP01086","properties":[{"key":"Description","value":"-"}]},{"database":"FunFam","id":"2.40.50.140:FF:000041","properties":[{"key":"EntryName","value":"Replication protein A subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.50.140:FF:000064","properties":[{"key":"EntryName","value":"Replication protein A subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.50.140:FF:000090","properties":[{"key":"EntryName","value":"Replication protein A subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.50.140:FF:000257","properties":[{"key":"EntryName","value":"Replication protein A subunit"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.50.140","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"4"}]},{"database":"InterPro","id":"IPR047192","properties":[{"key":"EntryName","value":"Euk_RPA1_DBD_C"}]},{"database":"InterPro","id":"IPR012340","properties":[{"key":"EntryName","value":"NA-bd_OB-fold"}]},{"database":"InterPro","id":"IPR013955","properties":[{"key":"EntryName","value":"Rep_factor-A_C"}]},{"database":"InterPro","id":"IPR007199","properties":[{"key":"EntryName","value":"Rep_factor-A_N"}]},{"database":"InterPro","id":"IPR031657","properties":[{"key":"EntryName","value":"REPA_OB_2"}]},{"database":"InterPro","id":"IPR004591","properties":[{"key":"EntryName","value":"Rfa1"}]},{"database":"InterPro","id":"IPR003871","properties":[{"key":"EntryName","value":"RFA1B/D_OB_1st"}]},{"database":"NCBIfam","id":"TIGR00617","properties":[{"key":"EntryName","value":"rpa1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47165","properties":[{"key":"EntryName","value":"OS03G0429900 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47165:SF4","properties":[{"key":"EntryName","value":"OS03G0429900 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02721","properties":[{"key":"EntryName","value":"DUF223"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04057","properties":[{"key":"EntryName","value":"Rep-A_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08646","properties":[{"key":"EntryName","value":"Rep_fac-A_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16900","properties":[{"key":"EntryName","value":"REPA_OB_2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50249","properties":[{"key":"EntryName","value":"Nucleic acid-binding proteins"},{"key":"MatchStatus","value":"4"}]}],"sequence":{"value":"MDSDAAPSVTPGAVAFVLENASPDAATGVPVPEIVLQVVDLKPIGTRFTFLASDGKDKIKTMLLTQLAPEVRSGNIQNLGVIRVLDYTCNTIGEKQEKVLIITKLEVVFKALDSEIKCEAEKQEEKPAILLSPKEESVVLSKPTNAPPLPPVVLKPKQEVKSASQIVNEQRGNAAPAARLAMTRRVHPLISLNPYQGNWIIKVRVTSKGNLRTYKNARGEGCVFNVELTDVDGTQIQATMFNEAAKKFYPMFELGKVYYISKGSLRVANKQFKTVHNDYEMTLNENAVVEEAEGETFIPQIQYNFVKIDQLGPYVGGRELVDVIGVVQSVSPTLSVRRKIDNETIPKRDIVVADDSSKTVTISLWNDLATTTGQELLDMVDSAPIIAIKSLKVSDFQGLSLSTVGRSTIVVNPDLPEAEQLRAWYDSEGKGTSMASIGSDMGASRVGGARSMYSDRVFLSHITSDPNLGQDKPVFFSLNAYISLIKPDQTMWYRACKTCNKKVTEAIGSGYWCEGCQKNDAECSLRYIMVIKVSDPTGEAWLSLFNDQAERIVGCSADELDRIRKEEGDDSYLLKLKEATWVPHLFRVSVTQNEYMNEKRQRITVRSEAPVDHAAEAKYMLEEIAKLTGC","length":630,"molWeight":69604,"crc64":"6593FE3B68125C20","md5":"1B06DDCCEACD2C530F3A746A225D1F7C"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"DNA binding":1,"Zinc finger":1,"Sequence conflict":2},"uniParcId":"UPI0000DB4529"}}