{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q0KHY3","secondaryAccessions":["B3DNA3","E1JJ26","E1JJ27","E2QD49","Q6NND0","Q8MR59"],"uniProtkbId":"MESH_DROME","entryAudit":{"firstPublicDate":"2008-12-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2013-10-16","entryVersion":131,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Drosophila melanogaster","commonName":"Fruit fly","taxonId":7227,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Panorpida","Diptera","Brachycera","Muscomorpha","Ephydroidea","Drosophilidae","Drosophila","Sophophora"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein mesh"}},"flag":"Precursor"},"genes":[{"geneName":{"value":"mesh"},"orfNames":[{"value":"CG31004"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"}],"value":"Required, together with Ssk and Tsp2A, for the proper organization of smooth septate junctions (sSJs), probably by mediating cell adhesion via its homophilic interaction (PubMed:22854041, PubMed:26848177). Also required for the correct subcellular localization of several sSJ components, such as Ssk, cora and l(2)gl, and for the barrier function of the midgut epithelium (PubMed:22854041). Required for maintaining the three-layered structure of the proventriculus (PubMed:22854041)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26848177"}],"value":"Forms a complex with Ssk and Tsp2A (PubMed:26848177). Interacts with Ssk; the interaction may be necessary for the localization of both proteins to the cell apicolateral region (PubMed:22854041)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Colocalizes with Ssk, cora, dlg1, l(2)gl and Fas3 at the apicolateral region of epithelial cells in the midgut. Also localizes to the apicolateral region of epithelial cells in the outer epithelial layer of the proventriculus and Malpighian tubules"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26848177"}],"value":"Cell junction, septate junction","id":"SL-0246"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26848177"}],"value":"Apicolateral cell membrane","id":"SL-0017"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"}],"value":"Single-pass type I membrane protein","id":"SL-9905"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"3"},"synonyms":[{"value":"D"}],"isoformIds":["Q0KHY3-3"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"1"},"synonyms":[{"value":"B"}],"isoformIds":["Q0KHY3-1"],"sequenceIds":["VSP_053282"],"isoformSequenceStatus":"Described"},{"name":{"value":"2"},"synonyms":[{"value":"C"}],"isoformIds":["Q0KHY3-2"],"sequenceIds":["VSP_035956"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26848177"}],"value":"At embryonic stage 12, expressed in endoderm-derived tissues (at protein level) (PubMed:22854041). In late-stage embryos, third-instar larvae and adult flies, expressed in the midgut, outer epithelial layer of the proventriculus and Malpighian tubules, but not expressed in the foregut and hindgut (at protein level) (PubMed:22854041, PubMed:26848177)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"}],"value":"Expressed throughout embryonic, larval and adult stages (at protein level)"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22854041"}],"value":"Homozygous larval lethal. Mutant embryos hatch into the first instar larvae, but die within 1 day. In mutant larvae midgut, a few septa are present at the cell contacts but large gaps between the lateral membranes of adjacent epithelial cells are frequently observed. Mutant larvae lack the three-layered structure of the proventriculus"}],"commentType":"DISRUPTION PHENOTYPE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AAM52621.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AAR96152.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":1454,"modifier":"EXACT"}},"description":"Protein mesh","featureId":"PRO_0000355637"},{"type":"Topological domain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":1186,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":1187,"modifier":"EXACT"},"end":{"value":1207,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":1208,"modifier":"EXACT"},"end":{"value":1454,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Domain","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":425,"modifier":"EXACT"}},"description":"NIDO","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00570"}]},{"type":"Domain","location":{"start":{"value":656,"modifier":"EXACT"},"end":{"value":807,"modifier":"EXACT"}},"description":"AMOP","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00347"}]},{"type":"Domain","location":{"start":{"value":820,"modifier":"EXACT"},"end":{"value":1028,"modifier":"EXACT"}},"description":"VWFD","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00580"}]},{"type":"Domain","location":{"start":{"value":1119,"modifier":"EXACT"},"end":{"value":1179,"modifier":"EXACT"}},"description":"Sushi","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00302"}]},{"type":"Region","location":{"start":{"value":1229,"modifier":"EXACT"},"end":{"value":1454,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1233,"modifier":"EXACT"},"end":{"value":1242,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1329,"modifier":"EXACT"},"end":{"value":1338,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1365,"modifier":"EXACT"},"end":{"value":1396,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1404,"modifier":"EXACT"},"end":{"value":1434,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":1249,"modifier":"EXACT"},"end":{"value":1249,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18327897"}]},{"type":"Modified residue","location":{"start":{"value":1251,"modifier":"EXACT"},"end":{"value":1251,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18327897"}]},{"type":"Modified residue","location":{"start":{"value":1254,"modifier":"EXACT"},"end":{"value":1254,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18327897"}]},{"type":"Modified residue","location":{"start":{"value":1295,"modifier":"EXACT"},"end":{"value":1295,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18327897"}]},{"type":"Modified residue","location":{"start":{"value":1297,"modifier":"EXACT"},"end":{"value":1297,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18327897"}]},{"type":"Glycosylation","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":320,"modifier":"EXACT"},"end":{"value":320,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":386,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":458,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":554,"modifier":"EXACT"},"end":{"value":554,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":620,"modifier":"EXACT"},"end":{"value":620,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":888,"modifier":"EXACT"},"end":{"value":888,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17893096"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":942,"modifier":"EXACT"},"end":{"value":942,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":977,"modifier":"EXACT"},"end":{"value":977,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":985,"modifier":"EXACT"},"end":{"value":985,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":1048,"modifier":"EXACT"},"end":{"value":1048,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":1066,"modifier":"EXACT"},"end":{"value":1066,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":1121,"modifier":"EXACT"},"end":{"value":1161,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Disulfide bond","location":{"start":{"value":1147,"modifier":"EXACT"},"end":{"value":1177,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Alternative sequence","location":{"start":{"value":1180,"modifier":"EXACT"},"end":{"value":1454,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.3"}],"featureId":"VSP_035956","alternativeSequence":{"originalSequence":"EVYYTRRVAFIAIGIIFLVICPLMLCIVCGVYRYRQKQLKEDPQWQMPMLPRSRASSARNLRTLNYDDDSDTDASTLKKSRSYDKVYRTNEPLPGKPQIDFPAKKWDLDENDEDVTSSEGEKPKQLGRRSLHSASGTELDQHGSPVDGDHPDEDDDFDEADVHTGTTHPVGYHQPLSPEEADQLHRQQYSPTFSGLDSRTSGASSINYTPQAATQNRFGGVPVLPSNTQYYSRPPSSVNTVPLRTAPTPLTQDTGLPSPPLATSPTPSVQKSTEV","alternativeSequences":["HEERSARVASISLGMVLLILIPCLLLLVCGASYWIRERIAREEEEAAQALLPKSKWQQSKQHVI"]}},{"type":"Alternative sequence","location":{"start":{"value":1259,"modifier":"EXACT"},"end":{"value":1282,"modifier":"EXACT"}},"description":"in isoform 1","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12537569"},{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.3"}],"featureId":"VSP_053282","alternativeSequence":{"originalSequence":"SRSYDKVYRTNEPLPGKPQIDFPA","alternativeSequences":["T"]}},{"type":"Sequence conflict","location":{"start":{"value":686,"modifier":"EXACT"},"end":{"value":686,"modifier":"EXACT"}},"description":"in Ref. 4; AAM52621","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["Y"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0130","category":"Biological process","name":"Cell adhesion"},{"id":"KW-0965","category":"Cellular component","name":"Cell junction"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0217","category":"Molecular function","name":"Developmental protein"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0768","category":"Domain","name":"Sushi"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"10731132","citationType":"journal article","authors":["Adams M.D.","Celniker S.E.","Holt R.A.","Evans C.A.","Gocayne J.D.","Amanatides P.G.","Scherer S.E.","Li P.W.","Hoskins R.A.","Galle R.F.","George R.A.","Lewis S.E.","Richards S.","Ashburner M.","Henderson S.N.","Sutton G.G.","Wortman J.R.","Yandell M.D.","Zhang Q.","Chen L.X.","Brandon R.C.","Rogers Y.-H.C.","Blazej R.G.","Champe M.","Pfeiffer B.D.","Wan K.H.","Doyle C.","Baxter E.G.","Helt G.","Nelson C.R.","Miklos G.L.G.","Abril J.F.","Agbayani A.","An H.-J.","Andrews-Pfannkoch C.","Baldwin D.","Ballew R.M.","Basu A.","Baxendale J.","Bayraktaroglu L.","Beasley E.M.","Beeson K.Y.","Benos P.V.","Berman B.P.","Bhandari D.","Bolshakov S.","Borkova D.","Botchan M.R.","Bouck J.","Brokstein P.","Brottier P.","Burtis K.C.","Busam D.A.","Butler H.","Cadieu E.","Center A.","Chandra I.","Cherry J.M.","Cawley S.","Dahlke C.","Davenport L.B.","Davies P.","de Pablos B.","Delcher A.","Deng Z.","Mays A.D.","Dew I.","Dietz S.M.","Dodson K.","Doup L.E.","Downes M.","Dugan-Rocha S.","Dunkov B.C.","Dunn P.","Durbin K.J.","Evangelista C.C.","Ferraz C.","Ferriera S.","Fleischmann W.","Fosler C.","Gabrielian A.E.","Garg N.S.","Gelbart W.M.","Glasser K.","Glodek A.","Gong F.","Gorrell J.H.","Gu Z.","Guan P.","Harris M.","Harris N.L.","Harvey D.A.","Heiman T.J.","Hernandez J.R.","Houck J.","Hostin D.","Houston K.A.","Howland T.J.","Wei M.-H.","Ibegwam C.","Jalali M.","Kalush F.","Karpen G.H.","Ke Z.","Kennison J.A.","Ketchum K.A.","Kimmel B.E.","Kodira C.D.","Kraft C.L.","Kravitz S.","Kulp D.","Lai Z.","Lasko P.","Lei Y.","Levitsky A.A.","Li J.H.","Li Z.","Liang Y.","Lin X.","Liu X.","Mattei B.","McIntosh T.C.","McLeod M.P.","McPherson D.","Merkulov G.","Milshina N.V.","Mobarry C.","Morris J.","Moshrefi A.","Mount S.M.","Moy M.","Murphy B.","Murphy L.","Muzny D.M.","Nelson D.L.","Nelson D.R.","Nelson K.A.","Nixon K.","Nusskern D.R.","Pacleb J.M.","Palazzolo M.","Pittman G.S.","Pan S.","Pollard J.","Puri V.","Reese M.G.","Reinert K.","Remington K.","Saunders R.D.C.","Scheeler F.","Shen H.","Shue B.C.","Siden-Kiamos I.","Simpson M.","Skupski M.P.","Smith T.J.","Spier E.","Spradling A.C.","Stapleton M.","Strong R.","Sun E.","Svirskas R.","Tector C.","Turner R.","Venter E.","Wang A.H.","Wang X.","Wang Z.-Y.","Wassarman D.A.","Weinstock G.M.","Weissenbach J.","Williams S.M.","Woodage T.","Worley K.C.","Wu D.","Yang S.","Yao Q.A.","Ye J.","Yeh R.-F.","Zaveri J.S.","Zhan M.","Zhang G.","Zhao Q.","Zheng L.","Zheng X.H.","Zhong F.N.","Zhong W.","Zhou X.","Zhu S.C.","Zhu X.","Smith H.O.","Gibbs R.A.","Myers E.W.","Rubin G.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10731132"},{"database":"DOI","id":"10.1126/science.287.5461.2185"}],"title":"The genome sequence of Drosophila melanogaster.","publicationDate":"2000","journal":"Science","firstPage":"2185","lastPage":"2195","volume":"287"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12537572","citationType":"journal article","authors":["Misra S.","Crosby M.A.","Mungall C.J.","Matthews B.B.","Campbell K.S.","Hradecky P.","Huang Y.","Kaminker J.S.","Millburn G.H.","Prochnik S.E.","Smith C.D.","Tupy J.L.","Whitfield E.J.","Bayraktaroglu L.","Berman B.P.","Bettencourt B.R.","Celniker S.E.","de Grey A.D.N.J.","Drysdale R.A.","Harris N.L.","Richter J.","Russo S.","Schroeder A.J.","Shu S.Q.","Stapleton M.","Yamada C.","Ashburner M.","Gelbart W.M.","Rubin G.M.","Lewis S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537572"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0083"}],"title":"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0083.1","lastPage":"RESEARCH0083.22","volume":"3"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-F45BMLFMHNBI5","citationType":"submission","authors":["Stapleton M.","Carlson J.W.","Booth B.","Chavez C.","Frise E.","George R.A.","Pacleb J.M.","Park S.","Wan K.H.","Yu C.","Rubin G.M.","Celniker S.E."],"publicationDate":"JAN-2012","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"12537569","citationType":"journal article","authors":["Stapleton M.","Carlson J.W.","Brokstein P.","Yu C.","Champe M.","George R.A.","Guarin H.","Kronmiller B.","Pacleb J.M.","Park S.","Wan K.H.","Rubin G.M.","Celniker S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537569"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0080"}],"title":"A Drosophila full-length cDNA resource.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0080.1","lastPage":"RESEARCH0080.8","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 217-1431 (ISOFORM 1)"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"},{"value":"Head","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"17893096","citationType":"journal article","authors":["Koles K.","Lim J.-M.","Aoki K.","Porterfield M.","Tiemeyer M.","Wells L.","Panin V."],"citationCrossReferences":[{"database":"PubMed","id":"17893096"},{"database":"DOI","id":"10.1093/glycob/cwm097"}],"title":"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster.","publicationDate":"2007","journal":"Glycobiology","firstPage":"1388","lastPage":"1403","volume":"17"},"referencePositions":["GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-888","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"Oregon-R","type":"STRAIN"},{"value":"Head","type":"TISSUE"}]},{"referenceNumber":6,"citation":{"id":"18327897","citationType":"journal article","authors":["Zhai B.","Villen J.","Beausoleil S.A.","Mintseris J.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"18327897"},{"database":"DOI","id":"10.1021/pr700696a"}],"title":"Phosphoproteome analysis of Drosophila melanogaster embryos.","publicationDate":"2008","journal":"J. Proteome Res.","firstPage":"1675","lastPage":"1682","volume":"7"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1249; THR-1251; SER-1254; THR-1295 AND SER-1297","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"Embryo","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"22854041","citationType":"journal article","authors":["Izumi Y.","Yanagihashi Y.","Furuse M."],"citationCrossReferences":[{"database":"PubMed","id":"22854041"},{"database":"DOI","id":"10.1242/jcs.112243"}],"title":"A novel protein complex, Mesh-Ssk, is required for septate junction formation in the Drosophila midgut.","publicationDate":"2012","journal":"J. Cell Sci.","firstPage":"4923","lastPage":"4933","volume":"125"},"referencePositions":["FUNCTION","INTERACTION WITH SSK","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","DEVELOPMENTAL STAGE","DISRUPTION PHENOTYPE"]},{"referenceNumber":8,"citation":{"id":"26848177","citationType":"journal article","authors":["Izumi Y.","Motoishi M.","Furuse K.","Furuse M."],"citationCrossReferences":[{"database":"PubMed","id":"26848177"},{"database":"DOI","id":"10.1242/jcs.180448"}],"title":"A tetraspanin regulates septate junction formation in Drosophila midgut.","publicationDate":"2016","journal":"J. Cell Sci.","firstPage":"1155","lastPage":"1164","volume":"129"},"referencePositions":["FUNCTION","IDENTIFICATION IN A COMPLEX WITH SSK AND TSP2A","SUBCELLULAR LOCATION","TISSUE SPECIFICITY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE014297","properties":[{"key":"ProteinId","value":"AAF57124.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AE014297","properties":[{"key":"ProteinId","value":"ACZ95074.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AE014297","properties":[{"key":"ProteinId","value":"ACZ95075.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AE014297","properties":[{"key":"ProteinId","value":"AAF57123.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BT032891","properties":[{"key":"ProteinId","value":"ACD99455.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT133164","properties":[{"key":"ProteinId","value":"AEZ02857.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT011360","properties":[{"key":"ProteinId","value":"AAR96152.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY122109","properties":[{"key":"ProteinId","value":"AAM52621.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001163781.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001170310.1"}],"isoformId":"Q0KHY3-2"},{"database":"RefSeq","id":"NP_001163782.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001170311.1"}],"isoformId":"Q0KHY3-3"},{"database":"RefSeq","id":"NP_001287618.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001300689.1"}],"isoformId":"Q0KHY3-2"},{"database":"RefSeq","id":"NP_733418.1","properties":[{"key":"NucleotideSequenceId","value":"NM_170539.1"}],"isoformId":"Q0KHY3-1"},{"database":"RefSeq","id":"NP_733419.1","properties":[{"key":"NucleotideSequenceId","value":"NM_170540.1"}],"isoformId":"Q0KHY3-1"},{"database":"AlphaFoldDB","id":"Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"68533","properties":[{"key":"Interactions","value":"7"}]},{"database":"FunCoup","id":"Q0KHY3","properties":[{"key":"Number of interactors","value":"6"}]},{"database":"IntAct","id":"Q0KHY3","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"7227.FBpp0290183","properties":[{"key":"Description","value":"-"}]},{"database":"GlyCosmos","id":"Q0KHY3","properties":[{"key":"glycosylation","value":"13 sites, No reported glycans"}]},{"database":"GlyGen","id":"Q0KHY3","properties":[{"key":"glycosylation","value":"15 sites, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"7227-FBpp0290183","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"43688","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"FBtr0300961","properties":[{"key":"ProteinId","value":"FBpp0290183"},{"key":"GeneId","value":"FBgn0051004"}]},{"database":"GeneID","id":"43688","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"dme:Dmel_CG31004","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"CG31004-RA","properties":[{"key":"OrganismName","value":"d. melanogaster"}],"isoformId":"Q0KHY3-3"},{"database":"UCSC","id":"CG31004-RB","properties":[{"key":"OrganismName","value":"d. melanogaster"}]},{"database":"AGR","id":"FB:FBgn0051004","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"43688","properties":[{"key":"Description","value":"-"}]},{"database":"FlyBase","id":"FBgn0051004","properties":[{"key":"GeneName","value":"mesh"}]},{"database":"VEuPathDB","id":"VectorBase:FBgn0051004","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4291","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FYYWRRM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"6051552at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-DME-913709","properties":[{"key":"PathwayName","value":"O-linked glycosylation of mucins"}]},{"database":"Reactome","id":"R-DME-977068","properties":[{"key":"PathwayName","value":"Termination of O-glycan biosynthesis"}]},{"database":"SignaLink","id":"Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"43688","properties":[{"key":"hits","value":"0 hits in 1 CRISPR screen"}]},{"database":"GenomeRNAi","id":"43688","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q0KHY3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000803","properties":[{"key":"Component","value":"Chromosome 3R"}]},{"database":"Bgee","id":"FBgn0051004","properties":[{"key":"ExpressionPatterns","value":"Expressed in adult differentiating enterocyte in digestive tract and 46 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q0KHY3","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0016327","properties":[{"key":"GoTerm","value":"C:apicolateral plasma membrane"},{"key":"GoEvidenceType","value":"IDA:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33589496"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005920","properties":[{"key":"GoTerm","value":"C:smooth septate junction"},{"key":"GoEvidenceType","value":"IDA:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22854041"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33589496"}]},{"database":"GO","id":"GO:0007160","properties":[{"key":"GoTerm","value":"P:cell-matrix adhesion"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090528","properties":[{"key":"GoTerm","value":"P:smooth septate junction assembly"},{"key":"GoEvidenceType","value":"IMP:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22854041"}]},{"database":"CDD","id":"cd00033","properties":[{"key":"EntryName","value":"CCP"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.10.70.10","properties":[{"key":"EntryName","value":"Complement Module, domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005533","properties":[{"key":"EntryName","value":"AMOP_dom"}]},{"database":"InterPro","id":"IPR056619","properties":[{"key":"EntryName","value":"C8-3_MUC4"}]},{"database":"InterPro","id":"IPR051495","properties":[{"key":"EntryName","value":"Epithelial_Barrier/Signaling"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR014756","properties":[{"key":"EntryName","value":"Ig_E-set"}]},{"database":"InterPro","id":"IPR003886","properties":[{"key":"EntryName","value":"NIDO_dom"}]},{"database":"InterPro","id":"IPR035976","properties":[{"key":"EntryName","value":"Sushi/SCR/CCP_sf"}]},{"database":"InterPro","id":"IPR000436","properties":[{"key":"EntryName","value":"Sushi_SCR_CCP_dom"}]},{"database":"InterPro","id":"IPR001846","properties":[{"key":"EntryName","value":"VWF_type-D"}]},{"database":"PANTHER","id":"PTHR13802","properties":[{"key":"EntryName","value":"MUCIN 4-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13802:SF52","properties":[{"key":"EntryName","value":"MUCIN-4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03782","properties":[{"key":"EntryName","value":"AMOP"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF23263","properties":[{"key":"EntryName","value":"C8-3_MUC4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06119","properties":[{"key":"EntryName","value":"NIDO"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00084","properties":[{"key":"EntryName","value":"Sushi"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00094","properties":[{"key":"EntryName","value":"VWD"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00723","properties":[{"key":"EntryName","value":"AMOP"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00032","properties":[{"key":"EntryName","value":"CCP"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00539","properties":[{"key":"EntryName","value":"NIDO"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00216","properties":[{"key":"EntryName","value":"VWD"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF57535","properties":[{"key":"EntryName","value":"Complement control module/SCR domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81296","properties":[{"key":"EntryName","value":"E set domains"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50856","properties":[{"key":"EntryName","value":"AMOP"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51220","properties":[{"key":"EntryName","value":"NIDO"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50923","properties":[{"key":"EntryName","value":"SUSHI"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51233","properties":[{"key":"EntryName","value":"VWFD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRFKLFVVCALACGFMAFVLANENISTEEFEDQIINAVPEPVKVTKPKAQAEFVEVPKATPAPKKAEADNPKTKPLALQKPTLIPVVHVSSRGTDDILTVAGLKPEKMFGALIMPNDYLGELYDVDNSGYNWDPNNNVAPPTTYSTAAGGYTITAARLAELRSNFMYWYFDKDMYGGRGDYQFDIHASMTQLHKNLNFQLPFYGFRFNYTRLSLNGYLEFSDPPEYLTYPLVFPIKDWPAKRDPSFMGIFFSKCRVGRIYPSDIDQRTPGVYFRVERDLMGRTDRFGVEVRERTMWDIRQGVVGADTFIPKHVVIATWKNVSFAGGIDNSLYTTNTFQMVLATDEVYTYIIFNYAVLNWLSHTEAGGDTTKGEGGVPAYVGFNAGNGTQAYEYNPYSQNMVIRDLANRGWANGFPGRHIFRVDEQILIGSCNKDIDAALLPLTFAPESGNMLGGQVVNITGPCFDPAIRVTCHFDTESVLGTYVDRNRVICVQPYLKAEGYIRFQISVGTQRFKWRGKYFVETPAAATEKIFFTTDDVHKKNPAEIRITWNQYNLTSNANANVMISLWGYRETKIEPQLEYIDVIEASYSNSGSYVITPSNYINRNNINRDMQFGFLQINLTQPDQYSGLAISPVLWSRPIPLAWYMAPQWERQHGKRWARALCDNWIRADRFLRNFAADLPLCPCTLDQAVLDKGRFRPDRECDKDSNPSCLRHRGAIHCVVSGTPVAQGAEQQCCYDRYGFLMLTYDQMWGSRPRRVHNLGKMPWNEASKVPSLSMWFHDMRPFYSCCYWQEEQAVGCETYRFERRPSQDCVAYQAPGIAGVFGDPHFVTFDGTAYTFNGLGEFVLARSVDESNKFEVQGRFEQLPVNYYGEVKATQLTAVAMRGNTTTTIEVRLRPLHARWRYRLDVLADGRRVYFDRESLKFQHFDGVTVYTPTYLLNQSQVVVQFDAGIGVEVVENEGYMTGRVFLPWKFINKTAGLFGNWSFNKLDDFMLPNGQVAQLNLNDLRSIHTNFGIKWMLTDREVPGVGAALFKREFGRMSGYYANATFQPNYVLDPADFLPANRSYDLERAEELCGECMQCQYDYAMTLNRDLAHFTKNYYDTIVNMQAENAVRVVSCGVLQTPRFGRKLSFDFMPGAKVSFECNEGFILIGDQRRECLSNGLWNIPEYGYTECLREVYYTRRVAFIAIGIIFLVICPLMLCIVCGVYRYRQKQLKEDPQWQMPMLPRSRASSARNLRTLNYDDDSDTDASTLKKSRSYDKVYRTNEPLPGKPQIDFPAKKWDLDENDEDVTSSEGEKPKQLGRRSLHSASGTELDQHGSPVDGDHPDEDDDFDEADVHTGTTHPVGYHQPLSPEEADQLHRQQYSPTFSGLDSRTSGASSINYTPQAATQNRFGGVPVLPSNTQYYSRPPSSVNTVPLRTAPTPLTQDTGLPSPPLATSPTPSVQKSTEV","length":1454,"molWeight":165038,"crc64":"2C8C1239C69EA6F8","md5":"3D7C837E2A3680F92B8CCD843F661EAA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":3,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"DISRUPTION PHENOTYPE":1,"SEQUENCE CAUTION":2},"countByFeatureType":{"Signal":1,"Chain":1,"Topological domain":2,"Transmembrane":1,"Domain":4,"Region":1,"Compositional bias":4,"Modified residue":5,"Glycosylation":13,"Disulfide bond":2,"Alternative sequence":2,"Sequence conflict":1},"uniParcId":"UPI0001506296"}}