{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q09232","uniProtkbId":"ORAI_CAEEL","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1997-11-01","entryVersion":137,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein orai"}},"alternativeNames":[{"fullName":{"value":"Store-operated calcium channel"}}]},"genes":[{"geneName":{"value":"orai-1"},"orfNames":[{"value":"C09F5.2"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17218360"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17376526"}],"value":"Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, stim-1. Required for Ca(2+) and IP3-dependent contractile activity of sheath cells and the spermatheca. Affects brood size and somatic cell function"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17218360"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17376526"}],"value":"Expressed in gonad sheath cells, hypodermis, intestine and spermatheca. Coexpressed with stim-1"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17218360"}],"value":"Worms exhibit an inhibition of intestinal Ca(2+) release activated Ca(2+) channel activity but has no effect on the initiation of posterior body wall muscle contraction, intestinal Ca(2+) oscillations or intestinal ER Ca(2+) store homeostasis. Attenuation also affects sterility and brood size"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Orai family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Protein orai","featureId":"PRO_0000065170"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":122,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":168,"modifier":"EXACT"},"end":{"value":198,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":199,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9851916","citationType":"journal article","authoringGroup":["The C. elegans sequencing consortium"],"citationCrossReferences":[{"database":"PubMed","id":"9851916"},{"database":"DOI","id":"10.1126/science.282.5396.2012"}],"title":"Genome sequence of the nematode C. elegans: a platform for investigating biology.","publicationDate":"1998","journal":"Science","firstPage":"2012","lastPage":"2018","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Bristol N2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"17376526","citationType":"journal article","authors":["Strange K.","Yan X.","Lorin-Nebel C.","Xing J."],"citationCrossReferences":[{"database":"PubMed","id":"17376526"},{"database":"DOI","id":"10.1016/j.ceca.2007.02.007"}],"title":"Physiological roles of STIM1 and Orai1 homologs and CRAC channels in the genetic model organism Caenorhabditis elegans.","publicationDate":"2007","journal":"Cell Calcium","firstPage":"193","lastPage":"203","volume":"42"},"referencePositions":["FUNCTION","TISSUE SPECIFICITY"]},{"referenceNumber":3,"citation":{"id":"17218360","citationType":"journal article","authors":["Lorin-Nebel C.","Xing J.","Yan X.","Strange K."],"citationCrossReferences":[{"database":"PubMed","id":"17218360"},{"database":"DOI","id":"10.1113/jphysiol.2006.124883"}],"title":"CRAC channel activity in C. elegans is mediated by Orai1 and STIM1 homologues and is essential for ovulation and fertility.","publicationDate":"2007","journal":"J. Physiol. (Lond.)","firstPage":"67","lastPage":"85","volume":"580"},"referencePositions":["FUNCTION","TISSUE SPECIFICITY","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"FO080475","properties":[{"key":"ProteinId","value":"CCD63980.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T15476","properties":[{"key":"EntryName","value":"T15476"}]},{"database":"RefSeq","id":"NP_001254834.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001267905.6"}]},{"database":"AlphaFoldDB","id":"Q09232","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q09232","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"40492","properties":[{"key":"Interactions","value":"1"}]},{"database":"DIP","id":"DIP-25585N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q09232","properties":[{"key":"Number of interactors","value":"420"}]},{"database":"IntAct","id":"Q09232","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"6239.C09F5.2a.1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"6239-C09F5.2a.1","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q09232","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"C09F5.2b.1","properties":[{"key":"ProteinId","value":"C09F5.2b.1"},{"key":"GeneId","value":"WBGene00015648"}]},{"database":"GeneID","id":"175221","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cel:CELE_C09F5.2","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"WB:WBGene00015648","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"175221","properties":[{"key":"Description","value":"-"}]},{"database":"WormBase","id":"C09F5.2b","properties":[{"key":"ProteinId","value":"CE01774"},{"key":"GeneId","value":"WBGene00015648"},{"key":"GeneName","value":"orai-1"}]},{"database":"eggNOG","id":"KOG4298","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_940845_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q09232","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"61124at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q09232","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q09232","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001940","properties":[{"key":"Component","value":"Chromosome III"}]},{"database":"Bgee","id":"WBGene00015648","properties":[{"key":"ExpressionPatterns","value":"Expressed in material anatomical entity and 5 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q09232","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0016324","properties":[{"key":"GoTerm","value":"C:apical plasma membrane"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17218360"}]},{"database":"GO","id":"GO:0016323","properties":[{"key":"GoTerm","value":"C:basolateral plasma membrane"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17218360"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17218360"}]},{"database":"GO","id":"GO:0005891","properties":[{"key":"GoTerm","value":"C:voltage-gated calcium channel complex"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"17376526"}]},{"database":"GO","id":"GO:0015279","properties":[{"key":"GoTerm","value":"F:store-operated calcium channel activity"},{"key":"GoEvidenceType","value":"IDA:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17218360"}]},{"database":"GO","id":"GO:0045087","properties":[{"key":"GoTerm","value":"P:innate immune response"},{"key":"GoEvidenceType","value":"HEP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0007007","source":"PubMed","id":"16968778"}]},{"database":"GO","id":"GO:0030728","properties":[{"key":"GoTerm","value":"P:ovulation"},{"key":"GoEvidenceType","value":"IMP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"17218360"}]},{"database":"GO","id":"GO:0019953","properties":[{"key":"GoTerm","value":"P:sexual reproduction"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"17376526"}]},{"database":"GO","id":"GO:0002115","properties":[{"key":"GoTerm","value":"P:store-operated calcium entry"},{"key":"GoEvidenceType","value":"IMP:WormBase"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"17218360"}]},{"database":"FunFam","id":"1.20.140.140:FF:000003","properties":[{"key":"EntryName","value":"Protein orai"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.140.140","properties":[{"key":"EntryName","value":"Calcium release-activated calcium channel protein Orai"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR012446","properties":[{"key":"EntryName","value":"CRAC_channel"}]},{"database":"InterPro","id":"IPR038350","properties":[{"key":"EntryName","value":"Orai_sf"}]},{"database":"PANTHER","id":"PTHR31501","properties":[{"key":"EntryName","value":"CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31501:SF7","properties":[{"key":"EntryName","value":"CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07856","properties":[{"key":"EntryName","value":"Orai-1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPRSHDPSRVELLRKEGGLTEKRVSISVEDIRGAVANWKNSGGAGDPITPYPLPQFFLQPPSTAGGGSRNGVGSKEGSVTSLRMPLKKAGDDVDLGHRGELDLSEKYNYDLSRAQLKASSRTSALLAGFAMVCLVELQYDQSTPKPLLIVLGVVTSLLVSVHLLALMMSTCILPYMEATGCTQDSPHIKLKFYIDLSWLFSTCIGLLLFLVEIGVIFYVKFTAVGYPTAGYITTAMLVPVGVVFVVFSYLIHKNRVSHSLGRFKHKVDTMKQFLDVEANLQKSTLAPSTIRDI","length":293,"molWeight":32024,"crc64":"41267F1B729CDD92","md5":"E933ED66D0F027C14CB4AD930F1E5F30"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":5,"Transmembrane":4,"Region":1},"uniParcId":"UPI000013BE87"}}