{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q08930","secondaryAccessions":["D6W3H7"],"uniProtkbId":"YP191_YEAST","entryAudit":{"firstPublicDate":"2006-05-30","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-11-01","entryVersion":148,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27292798"}],"value":"Ubiquitin carboxyl-terminal hydrolase MIY1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27292798"}],"value":"3.4.19.12"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27292798"}],"value":"Deubiquitinating enzyme MIY1"}}]},"genes":[{"orderedLocusNames":[{"value":"YPL191C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27292798"}],"value":"Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. Has endodeubiquitinase activity"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).","ecNumber":"3.4.19.12","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27292798"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562095"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 2050 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the MINDY deubiquitinase family. FAM63 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"Ubiquitin carboxyl-terminal hydrolase MIY1","featureId":"PRO_0000238658"},{"type":"Region","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":325,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]},{"type":"Active site","location":{"start":{"value":216,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]},{"type":"Site","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"Ubiquitin-binding","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]},{"type":"Site","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"Ubiquitin-binding","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]},{"type":"Site","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":303,"modifier":"EXACT"}},"description":"Ubiquitin-binding","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0788","category":"Molecular function","name":"Thiol protease"},{"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"}],"references":[{"referenceNumber":1,"citation":{"id":"9169875","citationType":"journal article","authors":["Bussey H.","Storms R.K.","Ahmed A.","Albermann K.","Allen E.","Ansorge W.","Araujo R.","Aparicio A.","Barrell B.G.","Badcock K.","Benes V.","Botstein D.","Bowman S.","Brueckner M.","Carpenter J.","Cherry J.M.","Chung E.","Churcher C.M.","Coster F.","Davis K.","Davis R.W.","Dietrich F.S.","Delius H.","DiPaolo T.","Dubois E.","Duesterhoeft A.","Duncan M.","Floeth M.","Fortin N.","Friesen J.D.","Fritz C.","Goffeau A.","Hall J.","Hebling U.","Heumann K.","Hilbert H.","Hillier L.W.","Hunicke-Smith S.","Hyman R.W.","Johnston M.","Kalman S.","Kleine K.","Komp C.","Kurdi O.","Lashkari D.","Lew H.","Lin A.","Lin D.","Louis E.J.","Marathe R.","Messenguy F.","Mewes H.-W.","Mirtipati S.","Moestl D.","Mueller-Auer S.","Namath A.","Nentwich U.","Oefner P.","Pearson D.","Petel F.X.","Pohl T.M.","Purnelle B.","Rajandream M.A.","Rechmann S.","Rieger M.","Riles L.","Roberts D.","Schaefer M.","Scharfe M.","Scherens B.","Schramm S.","Schroeder M.","Sdicu A.-M.","Tettelin H.","Urrestarazu L.A.","Ushinsky S.","Vierendeels F.","Vissers S.","Voss H.","Walsh S.V.","Wambutt R.","Wang Y.","Wedler E.","Wedler H.","Winnett E.","Zhong W.-W.","Zollner A.","Vo D.H.","Hani J."],"citationCrossReferences":[{"database":"PubMed","id":"9169875"},{"database":"DOI","id":"10.1038/387s103"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.","publicationDate":"1997","journal":"Nature","firstPage":"103","lastPage":"105","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"14562095","citationType":"journal article","authors":["Huh W.-K.","Falvo J.V.","Gerke L.C.","Carroll A.S.","Howson R.W.","Weissman J.S.","O'Shea E.K."],"citationCrossReferences":[{"database":"PubMed","id":"14562095"},{"database":"DOI","id":"10.1038/nature02026"}],"title":"Global analysis of protein localization in budding yeast.","publicationDate":"2003","journal":"Nature","firstPage":"686","lastPage":"691","volume":"425"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":4,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":5,"citation":{"id":"27292798","citationType":"journal article","authors":["Abdul Rehman S.A.","Kristariyanto Y.A.","Choi S.Y.","Nkosi P.J.","Weidlich S.","Labib K.","Hofmann K.","Kulathu Y."],"citationCrossReferences":[{"database":"PubMed","id":"27292798"},{"database":"DOI","id":"10.1016/j.molcel.2016.05.009"}],"title":"MINDY-1 is a member of an evolutionarily conserved and structurally distinct new family of deubiquitinating enzymes.","publicationDate":"2016","journal":"Mol. Cell","firstPage":"146","lastPage":"155","volume":"63"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","GENE FAMILY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z73547","properties":[{"key":"ProteinId","value":"CAA97904.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006949","properties":[{"key":"ProteinId","value":"DAA11243.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S65210","properties":[{"key":"EntryName","value":"S65210"}]},{"database":"AlphaFoldDB","id":"Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"35992","properties":[{"key":"Interactions","value":"77"}]},{"database":"DIP","id":"DIP-2779N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q08930","properties":[{"key":"Number of interactors","value":"215"}]},{"database":"IntAct","id":"Q08930","properties":[{"key":"Interactions","value":"3"}]},{"database":"MINT","id":"Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YPL191C","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YPL191C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YPL191C_mRNA","properties":[{"key":"ProteinId","value":"YPL191C"},{"key":"GeneId","value":"YPL191C"}]},{"database":"KEGG","id":"sce:YPL191C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000006112","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000006112","properties":[{"key":"GeneName","value":"YPL191C"}]},{"database":"VEuPathDB","id":"FungiDB:YPL191C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2427","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_022566_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"HTRFSNE","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10261212at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-34084-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"855910","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:Q08930","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XVI"}]},{"database":"RNAct","id":"Q08930","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0071944","properties":[{"key":"GoTerm","value":"C:cell periphery"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"26928762"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"26928762"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0016807","properties":[{"key":"GoTerm","value":"F:cysteine-type carboxypeptidase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27292798"}]},{"database":"GO","id":"GO:0004843","properties":[{"key":"GoTerm","value":"F:cysteine-type deubiquitinase activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27292798"}]},{"database":"GO","id":"GO:1990380","properties":[{"key":"GoTerm","value":"F:K48-linked deubiquitinase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27292798"}]},{"database":"GO","id":"GO:0071444","properties":[{"key":"GoTerm","value":"P:cellular response to pheromone"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"39798877"}]},{"database":"GO","id":"GO:0071108","properties":[{"key":"GoTerm","value":"P:protein K48-linked deubiquitination"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27292798"}]},{"database":"InterPro","id":"IPR007518","properties":[{"key":"EntryName","value":"MINDY"}]},{"database":"InterPro","id":"IPR033979","properties":[{"key":"EntryName","value":"MINDY_domain"}]},{"database":"PANTHER","id":"PTHR18063","properties":[{"key":"EntryName","value":"NF-E2 INDUCIBLE PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR18063:SF6","properties":[{"key":"EntryName","value":"UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04424","properties":[{"key":"EntryName","value":"MINDY_DUB"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDLSFTTKSVKINGQNHRILLQNENGPCALLALANILILSPDHTRFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINPEFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADINCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFSTLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQQLSTDIERDFGTGNLLLEEIQQIENDKELAKQLQEQEQERVTKFEAKRKIHSHKKNSEIHAPVKKDKFKRRSSLLNAKASEKEKSECVVM","length":360,"molWeight":41032,"crc64":"6C345663C0B69491","md5":"EC973D1616AFB3AD6BA99194DE1E5606"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Compositional bias":2,"Active site":2,"Site":3},"uniParcId":"UPI000006B1FC"}}