{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q07876","uniProtkbId":"RSMH_BACSU","entryAudit":{"firstPublicDate":"1994-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-10-01","entryVersion":152,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"Ribosomal RNA small subunit methyltransferase H"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"2.1.1.199"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"16S rRNA m(4)C1402 methyltransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"rRNA (cytosine-N(4)-)-methyltransferase RsmH"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"rsmH"},"synonyms":[{"value":"mraW"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"8636036"}],"value":"yllC"},{"value":"ylxA"}],"orderedLocusNames":[{"value":"BSU15140"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:42928"},{"database":"Rhea","id":"RHEA-COMP:10286"},{"database":"Rhea","id":"RHEA-COMP:10287"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:74506"},{"database":"ChEBI","id":"CHEBI:82748"}],"ecNumber":"2.1.1.199","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8636036"}],"value":"Transcribed at a low level in exponential phase, it may be further induced in stationary phase. Part of the mraZ-rsmH-ftsL-pbpB operon"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8636036"}],"value":"No visible phenotype"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"value":"Belongs to the methyltransferase superfamily. RsmH family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Ribosomal RNA small subunit methyltransferase H","featureId":"PRO_0000108578"},{"type":"Region","location":{"start":{"value":287,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}},{"type":"Binding site","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59789"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01007"}],"ligand":{"name":"S-adenosyl-L-methionine","id":"ChEBI:CHEBI:59789"}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0698","category":"Biological process","name":"rRNA processing"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"8636036","citationType":"journal article","authors":["Daniel R.A.","Williams A.M.","Errington J."],"citationCrossReferences":[{"database":"PubMed","id":"8636036"},{"database":"DOI","id":"10.1128/jb.178.8.2343-2350.1996"}],"title":"A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.","publicationDate":"1996","journal":"J. Bacteriol.","firstPage":"2343","lastPage":"2350","volume":"178"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"8244929","citationType":"journal article","authors":["Yanouri A.","Daniel R.A.","Errington J.","Buchanan C.E."],"citationCrossReferences":[{"database":"PubMed","id":"8244929"},{"database":"DOI","id":"10.1128/jb.175.23.7604-7616.1993"}],"title":"Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis.","publicationDate":"1993","journal":"J. Bacteriol.","firstPage":"7604","lastPage":"7616","volume":"175"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-311"],"referenceComments":[{"value":"168","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z68230","properties":[{"key":"ProteinId","value":"CAA92525.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB13387.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"L09703","properties":[{"key":"ProteinId","value":"AAC36835.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Unassigned_DNA"}]},{"database":"PIR","id":"D69881","properties":[{"key":"EntryName","value":"D69881"}]},{"database":"RefSeq","id":"NP_389397.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003245724.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"Q07876","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q07876","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q07876","properties":[{"key":"Number of interactors","value":"548"}]},{"database":"STRING","id":"224308.BSU15140","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q07876","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU15140","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB13387","properties":[{"key":"ProteinId","value":"CAB13387"},{"key":"GeneId","value":"BSU_15140"}]},{"database":"GeneID","id":"936555","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU15140","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.1650","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG0275","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"Q07876","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9806637at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q07876","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU15140-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0071424","properties":[{"key":"GoTerm","value":"F:rRNA (cytosine-N4-)-methyltransferase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0070475","properties":[{"key":"GoTerm","value":"P:rRNA base methylation"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"FunFam","id":"1.10.150.170:FF:000001","properties":[{"key":"EntryName","value":"Ribosomal RNA small subunit methyltransferase H"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.150.170","properties":[{"key":"EntryName","value":"Putative methyltransferase TM0872, insert domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01007","properties":[{"key":"EntryName","value":"16SrRNA_methyltr_H"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002903","properties":[{"key":"EntryName","value":"RsmH"}]},{"database":"InterPro","id":"IPR023397","properties":[{"key":"EntryName","value":"SAM-dep_MeTrfase_MraW_recog"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"NCBIfam","id":"TIGR00006","properties":[{"key":"EntryName","value":"16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11265:SF0","properties":[{"key":"EntryName","value":"12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11265","properties":[{"key":"EntryName","value":"S-ADENOSYL-METHYLTRANSFERASE MRAW"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01795","properties":[{"key":"EntryName","value":"Methyltransf_5"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF004486","properties":[{"key":"EntryName","value":"MraW"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81799","properties":[{"key":"EntryName","value":"Putative methyltransferase TM0872, insert domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFQHKTVLLRETVDGLNIKPDGTYVDCTLGGAGHSTYLLQQLSEKGRLIAFDQDDTALQHAKEVLSDYKGQLILIKSNFRYLKEYLNEQGVTEVDGILFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSATLSAKEVVNEWRYEDLVRIFFKYGEEKFSKQIARKIEEARMKSPIQTTGQLVDLIKDAIPAPARRSGGHPAKRVFQAIRIAVNDELRVFEEALEQAIEVLKPGGRVSVITFHSLEDRICKTTFKEKSSLPELPPGLPVIPEEFEPELKLITRKPITASQEELEENNRARSAKLRIAEKRK","length":311,"molWeight":35305,"crc64":"C50C609129DFD4AD","md5":"C1496F1A82187035EF10D6472A0E4233"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Compositional bias":1,"Binding site":5},"uniParcId":"UPI0000060376"}}