{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q07833","uniProtkbId":"WAPA_BACSU","entryAudit":{"firstPublicDate":"1994-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-07-28","entryVersion":154,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"tRNA nuclease WapA"},"ecNumbers":[{"value":"3.1.-.-"}]},"alternativeNames":[{"fullName":{"value":"Cell wall-associated polypeptide CWBP200"},"shortNames":[{"value":"CWBP200"}]},{"fullName":{"value":"RNase WapA"}},{"fullName":{"value":"Toxin WapA"}},{"fullName":{"value":"Wall-associated protein"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"wapA"},"orderedLocusNames":[{"value":"BSU39230"}],"orfNames":[{"value":"N17G"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23572593"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. A site-specific general tRNA nuclease, the C-terminus (residues 2201-2334) removes 2 or 4 nucleotides from the 3' end of at least 4 tRNAs (upon expression in E.coli), possibly endonucleolytically. The nuclease activity is neutralized by expression of the cognate immunity protein WapI from the same strain, but not its homolog from 2 other B.subtilis strains. The C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene. Cell contact is necessary for growth inhibition (PubMed:23572593). Unlike the LXG toxin-immunity modules, WapAI mediates competition under shaking culture conditions (PubMed:34280190)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11987133"}],"value":"Detected in exponentially growing cells as many processing products, protein disappears upon entry into stationary phase with the concomitant appearance of smaller products. The large products persist in the absence of the extracellular serine protease Epr (PubMed:11987133)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10658653"}],"value":"Probably exported by a SecA-dependent pathway"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11987133"}],"value":"Secreted, cell wall","id":"SL-0041"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10658653"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"Constitutively and highly expressed on solid and in liquid medium, with or without biofilm formation, by 12 hours of culture. Repressed by DegU"}],"commentType":"INDUCTION"},{"texts":[{"value":"Has two ligand-binding domains; the N-terminus has three 101 AA repeats which are responsible for cell wall binding; the C-terminus consists of two blocks of residues with a conserved motif repeated 31 times"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11987133"}],"value":"Identified in the extracellular proteome as many processing products ranging from over 85 kDa to about 30 kDa. One of these probably starts on Ser-1726. Two forms are known as CWBP62 and CWBP105 (PubMed:11987133)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23572593"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34280190"}],"value":"A double wapA-wapI deletion strain is growth inhibited when cocultured with wild-type cells. When wapI is reintroduced it restores growth in a cognate toxin-dependent fashion (PubMed:23572593). Growth inhibition by wild-type cells is strongest on LB medium and less effective on media that promote biofilm formation (MSgg and 2xSGG) (PubMed:34280190)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the RHS/WapA nuclease family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Or 32","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":2334,"modifier":"EXACT"}},"description":"tRNA nuclease WapA","featureId":"PRO_0000022689"},{"type":"Repeat","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":605,"modifier":"EXACT"}},"description":"1-1"},{"type":"Repeat","location":{"start":{"value":636,"modifier":"EXACT"},"end":{"value":736,"modifier":"EXACT"}},"description":"1-2"},{"type":"Repeat","location":{"start":{"value":769,"modifier":"EXACT"},"end":{"value":869,"modifier":"EXACT"}},"description":"1-3"},{"type":"Repeat","location":{"start":{"value":1021,"modifier":"EXACT"},"end":{"value":1040,"modifier":"EXACT"}},"description":"2-1"},{"type":"Repeat","location":{"start":{"value":1032,"modifier":"EXACT"},"end":{"value":1065,"modifier":"EXACT"}},"description":"YD 1"},{"type":"Repeat","location":{"start":{"value":1042,"modifier":"EXACT"},"end":{"value":1061,"modifier":"EXACT"}},"description":"2-2"},{"type":"Repeat","location":{"start":{"value":1063,"modifier":"EXACT"},"end":{"value":1082,"modifier":"EXACT"}},"description":"2-3"},{"type":"Repeat","location":{"start":{"value":1075,"modifier":"EXACT"},"end":{"value":1103,"modifier":"EXACT"}},"description":"YD 2"},{"type":"Repeat","location":{"start":{"value":1083,"modifier":"EXACT"},"end":{"value":1102,"modifier":"EXACT"}},"description":"2-4"},{"type":"Repeat","location":{"start":{"value":1109,"modifier":"EXACT"},"end":{"value":1128,"modifier":"EXACT"}},"description":"2-5"},{"type":"Repeat","location":{"start":{"value":1129,"modifier":"EXACT"},"end":{"value":1148,"modifier":"EXACT"}},"description":"2-6"},{"type":"Repeat","location":{"start":{"value":1150,"modifier":"EXACT"},"end":{"value":1169,"modifier":"EXACT"}},"description":"2-7"},{"type":"Repeat","location":{"start":{"value":1174,"modifier":"EXACT"},"end":{"value":1193,"modifier":"EXACT"}},"description":"2-8"},{"type":"Repeat","location":{"start":{"value":1199,"modifier":"EXACT"},"end":{"value":1218,"modifier":"EXACT"}},"description":"2-9"},{"type":"Repeat","location":{"start":{"value":1219,"modifier":"EXACT"},"end":{"value":1238,"modifier":"EXACT"}},"description":"2-10"},{"type":"Repeat","location":{"start":{"value":1636,"modifier":"EXACT"},"end":{"value":1671,"modifier":"EXACT"}},"description":"YD 3"},{"type":"Repeat","location":{"start":{"value":1646,"modifier":"EXACT"},"end":{"value":1665,"modifier":"EXACT"}},"description":"2-11"},{"type":"Repeat","location":{"start":{"value":1667,"modifier":"EXACT"},"end":{"value":1686,"modifier":"EXACT"}},"description":"2-12"},{"type":"Repeat","location":{"start":{"value":1690,"modifier":"EXACT"},"end":{"value":1709,"modifier":"EXACT"}},"description":"2-13"},{"type":"Repeat","location":{"start":{"value":1711,"modifier":"EXACT"},"end":{"value":1730,"modifier":"EXACT"}},"description":"2-14"},{"type":"Repeat","location":{"start":{"value":1732,"modifier":"EXACT"},"end":{"value":1751,"modifier":"EXACT"}},"description":"2-15"},{"type":"Repeat","location":{"start":{"value":1753,"modifier":"EXACT"},"end":{"value":1772,"modifier":"EXACT"}},"description":"2-16"},{"type":"Repeat","location":{"start":{"value":1795,"modifier":"EXACT"},"end":{"value":1814,"modifier":"EXACT"}},"description":"2-17"},{"type":"Repeat","location":{"start":{"value":1820,"modifier":"EXACT"},"end":{"value":1839,"modifier":"EXACT"}},"description":"2-18"},{"type":"Repeat","location":{"start":{"value":1840,"modifier":"EXACT"},"end":{"value":1859,"modifier":"EXACT"}},"description":"2-19"},{"type":"Repeat","location":{"start":{"value":1861,"modifier":"EXACT"},"end":{"value":1880,"modifier":"EXACT"}},"description":"2-20"},{"type":"Repeat","location":{"start":{"value":1887,"modifier":"EXACT"},"end":{"value":1906,"modifier":"EXACT"}},"description":"2-21"},{"type":"Repeat","location":{"start":{"value":1898,"modifier":"EXACT"},"end":{"value":1935,"modifier":"EXACT"}},"description":"YD 4"},{"type":"Repeat","location":{"start":{"value":1908,"modifier":"EXACT"},"end":{"value":1927,"modifier":"EXACT"}},"description":"2-22"},{"type":"Repeat","location":{"start":{"value":1929,"modifier":"EXACT"},"end":{"value":1948,"modifier":"EXACT"}},"description":"2-23"},{"type":"Repeat","location":{"start":{"value":1969,"modifier":"EXACT"},"end":{"value":1982,"modifier":"EXACT"}},"description":"2-24; approximate"},{"type":"Repeat","location":{"start":{"value":1983,"modifier":"EXACT"},"end":{"value":2002,"modifier":"EXACT"}},"description":"2-25"},{"type":"Repeat","location":{"start":{"value":2008,"modifier":"EXACT"},"end":{"value":2027,"modifier":"EXACT"}},"description":"2-26"},{"type":"Repeat","location":{"start":{"value":2028,"modifier":"EXACT"},"end":{"value":2047,"modifier":"EXACT"}},"description":"2-27"},{"type":"Repeat","location":{"start":{"value":2051,"modifier":"EXACT"},"end":{"value":2070,"modifier":"EXACT"}},"description":"2-28"},{"type":"Repeat","location":{"start":{"value":2071,"modifier":"EXACT"},"end":{"value":2090,"modifier":"EXACT"}},"description":"2-29"},{"type":"Repeat","location":{"start":{"value":2082,"modifier":"EXACT"},"end":{"value":2113,"modifier":"EXACT"}},"description":"YD 5"},{"type":"Repeat","location":{"start":{"value":2093,"modifier":"EXACT"},"end":{"value":2112,"modifier":"EXACT"}},"description":"2-30"},{"type":"Repeat","location":{"start":{"value":2120,"modifier":"EXACT"},"end":{"value":2139,"modifier":"EXACT"}},"description":"2-31"},{"type":"Region","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":869,"modifier":"EXACT"}},"description":"3 X 101 AA approximate tandem repeats"},{"type":"Region","location":{"start":{"value":1021,"modifier":"EXACT"},"end":{"value":2139,"modifier":"EXACT"}},"description":"31 X 21 AA approximate tandem repeats of X(4)-G-X(4)-[YF]-X-D-X(2)-G-X(4)"},{"type":"Region","location":{"start":{"value":2312,"modifier":"EXACT"},"end":{"value":2334,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":2315,"modifier":"EXACT"},"end":{"value":2334,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":1312,"modifier":"EXACT"},"end":{"value":1313,"modifier":"EXACT"}},"description":"in Ref. 1; AAA22883, 2; BAA06656 and 3; BAA06260","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"SG","alternativeSequences":["RR"]}}],"keywords":[{"id":"KW-0134","category":"Cellular component","name":"Cell wall"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"8316082","citationType":"journal article","authors":["Foster S.J."],"citationCrossReferences":[{"database":"PubMed","id":"8316082"},{"database":"DOI","id":"10.1111/j.1365-2958.1993.tb01574.x"}],"title":"Molecular analysis of three major wall-associated proteins of Bacillus subtilis 168: evidence for processing of the product of a gene encoding a 258 kDa precursor two-domain ligand-binding protein.","publicationDate":"1993","journal":"Mol. Microbiol.","firstPage":"299","lastPage":"310","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"7704263","citationType":"journal article","authors":["Yoshida K.","Sano H.","Seki S.","Oda M.","Fujimura M.","Fujita Y."],"citationCrossReferences":[{"database":"PubMed","id":"7704263"},{"database":"DOI","id":"10.1099/13500872-141-2-337"}],"title":"Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wapA loci.","publicationDate":"1995","journal":"Microbiology","firstPage":"337","lastPage":"343","volume":"141"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168 / BGSC1A1","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"8969509","citationType":"journal article","authors":["Yoshida K.","Shindo K.","Sano H.","Seki S.","Fujimura M.","Yanai N.","Miwa Y.","Fujita Y."],"citationCrossReferences":[{"database":"PubMed","id":"8969509"},{"database":"DOI","id":"10.1099/13500872-142-11-3113"}],"title":"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region.","publicationDate":"1996","journal":"Microbiology","firstPage":"3113","lastPage":"3123","volume":"142"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168 / BGSC1A1","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"19383706","citationType":"journal article","authors":["Barbe V.","Cruveiller S.","Kunst F.","Lenoble P.","Meurice G.","Sekowska A.","Vallenet D.","Wang T.","Moszer I.","Medigue C.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"19383706"},{"database":"DOI","id":"10.1099/mic.0.027839-0"}],"title":"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.","publicationDate":"2009","journal":"Microbiology","firstPage":"1758","lastPage":"1775","volume":"155"},"referencePositions":["SEQUENCE REVISION TO 1312-1313"]},{"referenceNumber":6,"citation":{"id":"10658653","citationType":"journal article","authors":["Hirose I.","Sano K.","Shioda I.","Kumano M.","Nakamura K.","Yamane K."],"citationCrossReferences":[{"database":"PubMed","id":"10658653"},{"database":"DOI","id":"10.1099/00221287-146-1-65"}],"title":"Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study.","publicationDate":"2000","journal":"Microbiology","firstPage":"65","lastPage":"75","volume":"146"},"referencePositions":["PROTEIN SEQUENCE OF 97-107","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"11987133","citationType":"journal article","authors":["Antelmann H.","Yamamoto H.","Sekiguchi J.","Hecker M."],"citationCrossReferences":[{"database":"PubMed","id":"11987133"},{"database":"DOI","id":"10.1002/1615-9861(200205)2:5<591::aid-prot591>3.0.co;2-8"}],"title":"Stabilization of cell wall proteins in Bacillus subtilis: a proteomic approach.","publicationDate":"2002","journal":"Proteomics","firstPage":"591","lastPage":"602","volume":"2"},"referencePositions":["ACTIVITY REGULATION","SUBCELLULAR LOCATION","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"23572593","citationType":"journal article","authors":["Koskiniemi S.","Lamoureux J.G.","Nikolakakis K.C.","t'Kint de Roodenbeke C.","Kaplan M.D.","Low D.A.","Hayes C.S."],"citationCrossReferences":[{"database":"PubMed","id":"23572593"},{"database":"DOI","id":"10.1073/pnas.1300627110"}],"title":"Rhs proteins from diverse bacteria mediate intercellular competition.","publicationDate":"2013","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"7032","lastPage":"7037","volume":"110"},"referencePositions":["FUNCTION AS A TRNA NUCLEASE","FUNCTION AS A TOXIN","EXPRESSION IN E.COLI","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":9,"citation":{"id":"34280190","citationType":"journal article","authors":["Kobayashi K."],"citationCrossReferences":[{"database":"PubMed","id":"34280190"},{"database":"DOI","id":"10.1371/journal.pgen.1009682"}],"title":"Diverse LXG toxin and antitoxin systems specifically mediate intraspecies competition in Bacillus subtilis biofilms.","publicationDate":"2021","journal":"PLoS Genet.","firstPage":"e1009682","lastPage":"e1009682","volume":"17"},"referencePositions":["INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / NRRL NRS-744 / VKM B-501","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L05634","properties":[{"key":"ProteinId","value":"AAA22883.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"D31856","properties":[{"key":"ProteinId","value":"BAA06656.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"D29985","properties":[{"key":"ProteinId","value":"BAA06260.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"D83026","properties":[{"key":"ProteinId","value":"BAA11683.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB15959.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S32920","properties":[{"key":"EntryName","value":"S32920"}]},{"database":"RefSeq","id":"NP_391802.2","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003243665.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"Q07833","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q07833","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q07833","properties":[{"key":"Number of interactors","value":"14"}]},{"database":"IntAct","id":"Q07833","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"224308.BSU39230","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q07833","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"224308-BSU39230","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB15959","properties":[{"key":"ProteinId","value":"CAB15959"},{"key":"GeneId","value":"BSU_39230"}]},{"database":"GeneID","id":"937525","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU39230","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.4247","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG3209","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"Q07833","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1432909at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU39230-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"Q07833","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10658653"}]},{"database":"GO","id":"GO:0016798","properties":[{"key":"GoTerm","value":"F:hydrolase activity, acting on glycosyl bonds"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004549","properties":[{"key":"GoTerm","value":"F:tRNA-specific ribonuclease activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23572593"}]},{"database":"GO","id":"GO:0016078","properties":[{"key":"GoTerm","value":"P:tRNA decay"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23572593"}]},{"database":"FunFam","id":"2.180.10.10:FF:000010","properties":[{"key":"EntryName","value":"Cell wall-associated protein"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.180.10.10:FF:000015","properties":[{"key":"EntryName","value":"Cell wall-associated protein"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.970","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.260","properties":[{"key":"EntryName","value":"Galactose-binding domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.180.10.10","properties":[{"key":"EntryName","value":"RHS repeat-associated core"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR055372","properties":[{"key":"EntryName","value":"CBM96"}]},{"database":"InterPro","id":"IPR003305","properties":[{"key":"EntryName","value":"CenC_carb-bd"}]},{"database":"InterPro","id":"IPR045351","properties":[{"key":"EntryName","value":"DUF6531"}]},{"database":"InterPro","id":"IPR008979","properties":[{"key":"EntryName","value":"Galactose-bd-like_sf"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR022385","properties":[{"key":"EntryName","value":"Rhs_assc_core"}]},{"database":"InterPro","id":"IPR031325","properties":[{"key":"EntryName","value":"RHS_repeat"}]},{"database":"InterPro","id":"IPR050708","properties":[{"key":"EntryName","value":"T6SS_VgrG/RHS"}]},{"database":"InterPro","id":"IPR056823","properties":[{"key":"EntryName","value":"TEN-like_YD-shell"}]},{"database":"InterPro","id":"IPR006530","properties":[{"key":"EntryName","value":"YD"}]},{"database":"NCBIfam","id":"NF033679","properties":[{"key":"EntryName","value":"DNRLRE_dom"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR03696","properties":[{"key":"EntryName","value":"Rhs_assc_core"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01643","properties":[{"key":"EntryName","value":"YD_repeat_2x"},{"key":"MatchStatus","value":"5"}]},{"database":"PANTHER","id":"PTHR32305","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32305:SF17","properties":[{"key":"EntryName","value":"TRNA NUCLEASE WAPA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF24517","properties":[{"key":"EntryName","value":"CBM96"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02018","properties":[{"key":"EntryName","value":"CBM_4_9"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20148","properties":[{"key":"EntryName","value":"DUF6531"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05593","properties":[{"key":"EntryName","value":"RHS_repeat"},{"key":"MatchStatus","value":"4"}]},{"database":"Pfam","id":"PF25023","properties":[{"key":"EntryName","value":"TEN_YD-shell"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF49785","properties":[{"key":"EntryName","value":"Galactose-binding domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKKRKRRNFKRFIAAFLVLALMISLVPADVLAKSTEEENGNRIAADDPEETLQKEQTEEAVPFDPKDINKEGEITSERTENTKLYYEGDGVYKQEVYLDPIHTKETPDADWEDISPELKESTSKQVETENAILNSDFQKQMKNGLYATFEHNDHKVTYSLAEAKGPNKTSLTPKDTSADYKTDSNEIVYPDVFPNIDLQTFTFNENIKEDLVLHQYNGYNTFTFQLKTDLQAKEQEDGSIDFSDEKGKVVFSVPKPFMTDSKLDELSGEVERSDKVSYKLEKNEEGYLLHLTADENWLKDPERVYPVSIDPSTSLSVSSDTFVMSAYPTTNYSASSQKWDANLKAYVLKTGYYDKTTGTNYAFMKFNNLKPIQNMTVTKATLKTYVAHSYYGTKATGLWLDTVNSNYDNAKVTWNTKPASKNIGKADVHKGQWASYDVTAAVKSWNSGGANYGFKLHTNGNGKEYWKKLISSANSANKPYIEVTYTIPKGNTPTIKAYHNGDSTGYFDISWKKVEGAKGYKVWIYNGKEYQAISAGNVTSWSTKGKKIWPTSAEIASKRYKLHLDGKDGAELALDPSPVYKNSGGSYATSKNYWIGVSAIFDQGEGAMSAPAKPVIPNVGKAQAPSAKGYNNGNATGYFDLSWKAVSGATGYKVQVFNGKGFETLDLGNQTSWTTKGKKIWPTSAEIKAGKYALHLKDGSGAELPINPGPTYKNAGGDGAKRNYSFKIIAYNKDGEAIASPAATPALPDIARPKNVTGYLYTNTKSSQTGYVNLIWEKVQNAKGYKVNIYNGKEYQSFDVGDADHWTTQNKNIWPTSEEIKAGSYKLHTDGKGGELALDPSPVYNNANGNYKGKKNYSFTLVAYDANGETIPTAPFNPTFHEGAEFLGTEEYWSIIDIPSGQLNGATGNVIVNEEDLSIDGRGPGLGLSRTYNSLDSSDHLFGQGWYADAETSVISTDGGAMYIDEDATTHRFTKKADGTYQPPTGVYLELTETADQFILKTKDQTNAYFNKKGGKLQKVVDGHNNATVYTYNDKNQLTAITDASGRKLTFTYDENGHVTSITGPKNKKVTYSYENDLLKKVTDTDGTVTSYDYDSEGRLVKQYSANSTEAKPVFTEYQYSGHRLEKAINAKKETYVYSYDADKKTLLMTQPNGRKVQYGYNEAGNPIQVIDDAEGLKITTNTKYEGNNVVEDVDPNDVGTGKATESYQYDKDGNVTSVKDAYGTETYEYNKNNDVTKMKDTEGNVTDIAYDGLDAVSETDQSGKSSSAAVYDKYGNQIQSSKDLSASTNILKDGSFEAQKSGWNLTASKDSGKISVIADKSGVLSGSKALEVLSQSTSAGTDHGYSSATQTVELEPNTTYTLSGKIKTDLAKSRAYFNIDLRDKDQKRIQWIHNEYSALAGKNDWTKRQITFTTPANAGKAVVYMEVDHKDKDGKGKAWFDEVQLEKGEVSSSYNPVQNSSFTSATENWNVSGASVDSEEGFNDDVSLKAARTSASQAGSVTKQTVVLGQSANDKPVYLTLTGMSKASSVKFTDEKDYSLQANVTYADGSTGIYNAKFPSGTQEWNRAAVVIPKTKPINKVDISILFQKSATGTVWFDDIRLIEGSLLTKSTYDSNGNYVTKEEDELGYATSTDYDETGKKTSETDAKGEKTTYTYDQADQLTNMTLSNGTSILHSYDKEGNEVSKTIRAGADQTYKFEYDVMGKLVKTTDPLGNVLASEYDANSNLTKTISPNGNEVSLSYDGTDRVKSKSYNGTEKYIFTYDKNGNETSVVNKEQNTTKKRTFDNKNRLTELTDRGGSQTWTYPSDSDKLKTFSWIHGDQKGTNQFTYNKLDQMIEMKDSTSSYSFDYDENGNVQTFITGNGGGTSFSYDERNLVSSLHIGDKNGGDILTESYEYDANGNRTTINSSASGKVQYEYGKLNQLVKETHEDGTVIEYTYDGFGNRKTVTTIKDGSSKTVNASFNIMNQLTKVNDESISYDKNGNRTSDGKFTYTWDAEDNLTAVTKKGEDKPFATYKYDEKGNRIQKTVNGKVTNYFYDGDSLNVLYETDADNNVTKSYTYGDSGQLLSYTENGKKYFYHYNAHGDIIAISDSTGKTVAKYQYDAWGNPTKTEASDEVKDNRYRYAGYQYDEETGLYYLMARYYEPRNGVFLSLDPDPGSDGDSLDQNGYAYGNNNPVMNVDPDGHWVWLVVNAGFAAYDGYKAYKSGKGWKGAAWAAASNFGPGKIFKGASRAYKFTKKAVKITGHTRHGLNQSIGRNGGRGVNLRAKLNAVRSPKKVIKQPNGATKYVGKKATVVLNKRGKVITAYGSSRAKGSKHVFHTHGKGNKSKRRR","length":2334,"molWeight":258162,"crc64":"DAE0897CD279AEB6","md5":"572B63E6743A83E568C0614872418F67"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"ACTIVITY REGULATION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":1,"PTM":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Repeat":39,"Region":4,"Compositional bias":1,"Sequence conflict":1},"uniParcId":"UPI000195C71B"}}