{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q05131","secondaryAccessions":["D6VZK9"],"uniProtkbId":"RCH1_YEAST","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":154,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26832117"}],"value":"Solute carrier RCH1"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26832117"}],"value":"Regulator of calcium homeostasis 1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26832117"}],"value":"RCH1"},"orderedLocusNames":[{"value":"YMR034C"}],"orfNames":[{"value":"YM9973.08C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26832117"}],"value":"Solute carrier protein that negatively regulates the cytosolic calcium homeostasis in response to high levels of extracellular calcium"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26832117"}],"value":"Distributes as multiple foci in the plasma membrane prior to cell division, moves toward and concentrates at the bud neck as the bud grows in size, and disperses again along the plasma membrane immediately prior to cytokinesis"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26832117"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26832117"}],"value":"Bud neck","id":"SL-0029"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26832117"}],"value":"Expression is positively regulated by calcium/calcineurin signaling through the sole CDRE element (5'-TTAGCCTC-3') in its promoter"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":434,"modifier":"EXACT"}},"description":"Solute carrier RCH1","featureId":"PRO_0000203274"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":285,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":328,"modifier":"EXACT"},"end":{"value":348,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":362,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":363,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":384,"modifier":"EXACT"},"end":{"value":434,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Modified residue","location":{"start":{"value":425,"modifier":"EXACT"},"end":{"value":425,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]}],"keywords":[{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-0109","category":"Biological process","name":"Calcium transport"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9169872","citationType":"journal article","authors":["Bowman S.","Churcher C.M.","Badcock K.","Brown D.","Chillingworth T.","Connor R.","Dedman K.","Devlin K.","Gentles S.","Hamlin N.","Hunt S.","Jagels K.","Lye G.","Moule S.","Odell C.","Pearson D.","Rajandream M.A.","Rice P.","Skelton J.","Walsh S.V.","Whitehead S.","Barrell B.G."],"citationCrossReferences":[{"database":"PubMed","id":"9169872"},{"database":"DOI","id":"10.1038/387s090"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.","publicationDate":"1997","journal":"Nature","firstPage":"90","lastPage":"93","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"17322287","citationType":"journal article","authors":["Hu Y.","Rolfs A.","Bhullar B.","Murthy T.V.S.","Zhu C.","Berger M.F.","Camargo A.A.","Kelley F.","McCarron S.","Jepson D.","Richardson A.","Raphael J.","Moreira D.","Taycher E.","Zuo D.","Mohr S.","Kane M.F.","Williamson J.","Simpson A.J.G.","Bulyk M.L.","Harlow E.","Marsischky G.","Kolodner R.D.","LaBaer J."],"citationCrossReferences":[{"database":"PubMed","id":"17322287"},{"database":"DOI","id":"10.1101/gr.6037607"}],"title":"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.","publicationDate":"2007","journal":"Genome Res.","firstPage":"536","lastPage":"543","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16847258","citationType":"journal article","authors":["Kim H.","Melen K.","Oesterberg M.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"16847258"},{"database":"DOI","id":"10.1073/pnas.0604075103"}],"title":"A global topology map of the Saccharomyces cerevisiae membrane proteome.","publicationDate":"2006","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"11142","lastPage":"11147","volume":"103"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"ATCC 208353 / W303-1A","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"19779198","citationType":"journal article","authors":["Holt L.J.","Tuch B.B.","Villen J.","Johnson A.D.","Gygi S.P.","Morgan D.O."],"citationCrossReferences":[{"database":"PubMed","id":"19779198"},{"database":"DOI","id":"10.1126/science.1172867"}],"title":"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.","publicationDate":"2009","journal":"Science","firstPage":"1682","lastPage":"1686","volume":"325"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-425","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"26832117","citationType":"journal article","authors":["Zhao Y.","Yan H.","Happeck R.","Peiter-Volk T.","Xu H.","Zhang Y.","Peiter E.","van Oostende Triplet C.","Whiteway M.","Jiang L."],"citationCrossReferences":[{"database":"PubMed","id":"26832117"},{"database":"DOI","id":"10.1016/j.ejcb.2016.01.001"}],"title":"The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast.","publicationDate":"2016","journal":"Eur. J. Cell Biol.","firstPage":"164","lastPage":"174","volume":"95"},"referencePositions":["FUNCTION","INDUCTION","SUBCELLULAR LOCATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z49213","properties":[{"key":"ProteinId","value":"CAA89150.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY692713","properties":[{"key":"ProteinId","value":"AAT92732.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006946","properties":[{"key":"ProteinId","value":"DAA09933.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S53951","properties":[{"key":"EntryName","value":"S53951"}]},{"database":"RefSeq","id":"NP_013748.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001182531.1"}]},{"database":"AlphaFoldDB","id":"Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"35206","properties":[{"key":"Interactions","value":"90"}]},{"database":"FunCoup","id":"Q05131","properties":[{"key":"Number of interactors","value":"574"}]},{"database":"MINT","id":"Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YMR034C","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YMR034C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YMR034C_mRNA","properties":[{"key":"ProteinId","value":"YMR034C"},{"key":"GeneId","value":"YMR034C"}]},{"database":"GeneID","id":"855050","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YMR034C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000004637","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000004637","properties":[{"key":"GeneName","value":"RCH1"}]},{"database":"VEuPathDB","id":"FungiDB:YMR034C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4821","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_039013_3_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"LPIMIYH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"188035at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-32739-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"855050","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:Q05131","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XIII"}]},{"database":"RNAct","id":"Q05131","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0071944","properties":[{"key":"GoTerm","value":"C:cell periphery"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"26928762"}]},{"database":"GO","id":"GO:0005935","properties":[{"key":"GoTerm","value":"C:cellular bud neck"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26832117"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26832117"}]},{"database":"GO","id":"GO:0008028","properties":[{"key":"GoTerm","value":"F:monocarboxylic acid transmembrane transporter activity"},{"key":"GoEvidenceType","value":"ISA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000247","source":"PubMed","id":"10543755"}]},{"database":"GO","id":"GO:0051481","properties":[{"key":"GoTerm","value":"P:negative regulation of cytosolic calcium ion concentration"},{"key":"GoEvidenceType","value":"IGI:SGD"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"26832117"}]},{"database":"Gene3D","id":"1.20.1530.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR038770","properties":[{"key":"EntryName","value":"Na+/solute_symporter_sf"}]},{"database":"InterPro","id":"IPR016833","properties":[{"key":"EntryName","value":"Put_Na-Bile_cotransptr"}]},{"database":"PANTHER","id":"PTHR18640:SF5","properties":[{"key":"EntryName","value":"SODIUM_BILE ACID COTRANSPORTER 7"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR18640","properties":[{"key":"EntryName","value":"SOLUTE CARRIER FAMILY 10 MEMBER 7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13593","properties":[{"key":"EntryName","value":"SBF_like"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF026166","properties":[{"key":"EntryName","value":"UCP026166"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKTQYSLIRKIWAHSVTEFLKSQWFFICLAILIVIARFAPNFARDGGLIKGQYSIGYGCVAWIFLQSGLGMKSRSLMANMLNWRAHATILVLSFLITSSIVYGFCCAVKAANDPKIDDWVLIGLILTATCPTTVASNVIMTTNAGGNSLLCVCEVFIGNLLGAFITPALVQMFTNRAPFAYGNPATGNGIGALYGRVMKQVGLSVFVPLFVGQVIQNCFPKGTAYYLGFLKKYHIKIGSYMLLLIMFSSFSTAFYQDAFTSVSHVCIIFLCFFNLGIYIFFTGLSYLCARPWFILKLFPHEPIEGKSTRLYRYSYNIFRPFYYSKEDAICIMFCGPAKTAALGVSLITSQYGDKKEHLGKLLVPLVLYQVEQVMTANFFVSLFKRWIQKDAQADGSESSCANENEEVDLEKIISIGTGENQSVLSNNVPYTQPR","length":434,"molWeight":48375,"crc64":"80922AC8D1BEE433","md5":"36DD34798B931B96D6909908DE23B0A4"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":11,"Transmembrane":10,"Modified residue":1},"uniParcId":"UPI000013BA1A"}}