{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q04471","secondaryAccessions":["D6VZT7"],"uniProtkbId":"YM04_YEAST","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":148,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Abasic site processing protein YMR114C"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"4.-.-.-"}]},"alternativeNames":[{"fullName":{"value":"Peptidase YMR114C"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8R1M0"}],"value":"3.4.-.-"}]}]},"genes":[{"orderedLocusNames":[{"value":"YMR114C"}],"orfNames":[{"value":"YM9718.13C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8R1M0"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The DNA-protein cross-link is then reversed: able to catalyze the reversal of the thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"Formation and reversal of DNA-protein cross-link depends on DNA context. Catalyzes formation of the thiazolidine linkage in presence of abasic sites in single-stranded DNA. Mediates the reversal of the thiazolidine cross-link in presence of double stranded DNA"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"Localizes to replication forks"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"Chromosome","id":"SL-0468"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P76318"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}],"value":"The N-terminal catalytic Cys-2 residue forms a thiazolidine linkage to a ring-opened DNA abasic site (By similarity). Glu-132 catalyzes reversal of the thiazolidine linkage; self-reversal is favoured by duplex DNA formation (By similarity)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the SOS response-associated peptidase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8R1M0"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"Abasic site processing protein YMR114C","featureId":"PRO_0000203292"},{"type":"Region","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":281,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":326,"modifier":"EXACT"},"end":{"value":349,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}]},{"type":"Active site","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}]},{"type":"Modified residue","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"Thiazolidine linkage to a ring-opened DNA abasic site","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96FZ2"}]},{"type":"Modified residue","location":{"start":{"value":338,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"17287358"}]}],"keywords":[{"id":"KW-0158","category":"Cellular component","name":"Chromosome"},{"id":"KW-0190","category":"PTM","name":"Covalent protein-DNA linkage"},{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9169872","citationType":"journal article","authors":["Bowman S.","Churcher C.M.","Badcock K.","Brown D.","Chillingworth T.","Connor R.","Dedman K.","Devlin K.","Gentles S.","Hamlin N.","Hunt S.","Jagels K.","Lye G.","Moule S.","Odell C.","Pearson D.","Rajandream M.A.","Rice P.","Skelton J.","Walsh S.V.","Whitehead S.","Barrell B.G."],"citationCrossReferences":[{"database":"PubMed","id":"9169872"},{"database":"DOI","id":"10.1038/387s090"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.","publicationDate":"1997","journal":"Nature","firstPage":"90","lastPage":"93","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"17287358","citationType":"journal article","authors":["Chi A.","Huttenhower C.","Geer L.Y.","Coon J.J.","Syka J.E.P.","Bai D.L.","Shabanowitz J.","Burke D.J.","Troyanskaya O.G.","Hunt D.F."],"citationCrossReferences":[{"database":"PubMed","id":"17287358"},{"database":"DOI","id":"10.1073/pnas.0607084104"}],"title":"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.","publicationDate":"2007","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"2193","lastPage":"2198","volume":"104"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z49702","properties":[{"key":"ProteinId","value":"CAA89751.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006946","properties":[{"key":"ProteinId","value":"DAA10011.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S54575","properties":[{"key":"EntryName","value":"S54575"}]},{"database":"RefSeq","id":"NP_013832.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001182614.1"}]},{"database":"AlphaFoldDB","id":"Q04471","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q04471","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"35290","properties":[{"key":"Interactions","value":"34"}]},{"database":"DIP","id":"DIP-5315N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q04471","properties":[{"key":"Number of interactors","value":"707"}]},{"database":"STRING","id":"4932.YMR114C","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q04471","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YMR114C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q04471","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YMR114C_mRNA","properties":[{"key":"ProteinId","value":"YMR114C"},{"key":"GeneId","value":"YMR114C"}]},{"database":"GeneID","id":"855141","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YMR114C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000004720","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000004720","properties":[{"key":"GeneName","value":"YMR114C"}]},{"database":"VEuPathDB","id":"FungiDB:YMR114C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2618","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_035990_0_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q04471","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MPCVLEP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2111841at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-32809-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"855141","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:Q04471","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XIII"}]},{"database":"RNAct","id":"Q04471","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005694","properties":[{"key":"GoTerm","value":"C:chromosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"24390141"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"}]},{"database":"GO","id":"GO:0005657","properties":[{"key":"GoTerm","value":"C:replication fork"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0140431","properties":[{"key":"GoTerm","value":"F:DNA-(abasic site) binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0160129","properties":[{"key":"GoTerm","value":"F:protein-DNA covalent cross-linking activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0003697","properties":[{"key":"GoTerm","value":"F:single-stranded DNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0097681","properties":[{"key":"GoTerm","value":"P:double-strand break repair via alternative nonhomologous end joining"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0036297","properties":[{"key":"GoTerm","value":"P:interstrand cross-link repair"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0106300","properties":[{"key":"GoTerm","value":"P:protein-DNA covalent cross-linking repair"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"Gene3D","id":"3.90.1680.10","properties":[{"key":"EntryName","value":"SOS response associated peptidase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003738","properties":[{"key":"EntryName","value":"SRAP"}]},{"database":"InterPro","id":"IPR036590","properties":[{"key":"EntryName","value":"SRAP-like"}]},{"database":"PANTHER","id":"PTHR13604:SF0","properties":[{"key":"EntryName","value":"ABASIC SITE PROCESSING PROTEIN HMCES"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13604","properties":[{"key":"EntryName","value":"DC12-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02586","properties":[{"key":"EntryName","value":"SRAP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF143081","properties":[{"key":"EntryName","value":"BB1717-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MCGRFALAYDSGDLPQLLRDWNLPVNTPKDASSNSQHPHDEEDTKDQPTVSKDIFKASYNISPTNYSAVYRPDTKAIQFMRWGLVPFWTKDVSQFKTYRTFNARLENLQESKMWMRPCEKKRCAVLMSGYFEWKTVGKKKTPYFISRRDGRLMFVAGMYDYVEKDDLYTFTIITAQGPRELEWLHERMPCVLEPGTESWDAWMDVDKTTWSTEELVKLLKPDYDESKLQFYQVTDDVGKTTNTGERLIKPLLKEDSDMFSVKREKEEALLENDNEQGIDNRGVKGDKSLKGEDVFNQKKSLKRNSYDGLKKNEEQEETTLPEEGSIGDRVKREEANLSPKREGNREKRNIVNMLGNQKDSRGKKKIKK","length":368,"molWeight":42901,"crc64":"0FC101AA7C918F7D","md5":"3E30A39AE29620AAE9DC19A95A5E71BD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"ACTIVITY REGULATION":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Region":2,"Compositional bias":4,"Active site":2,"Modified residue":2},"uniParcId":"UPI000013B884"}}