{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q02792","secondaryAccessions":["D6W2B4"],"uniProtkbId":"XRN2_YEAST","entryAudit":{"firstPublicDate":"1993-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":201,"sequenceVersion":3},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"5'-3' exoribonuclease 2"},"ecNumbers":[{"value":"3.1.13.-"}]},"alternativeNames":[{"fullName":{"value":"Ribonucleic acid-trafficking protein 1"}},{"fullName":{"value":"p116"}}]},"genes":[{"geneName":{"value":"RAT1"},"synonyms":[{"value":"HKE1"},{"value":"TAP1"}],"orderedLocusNames":[{"value":"YOR048C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10805743"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11030620"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11142370"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11238889"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12769851"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14561886"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15565157"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16131592"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16598041"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7608167"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8417335"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9207242"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9384595"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9488433"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9584178"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9891049"}],"value":"Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA, rRNA and small nucleolar RNA (snoRNA) precursors. May promote termination of transcription by RNA polymerase II via the recruitment of 3'-end processing factors to the poly(A) site and by the degradation of nascent RNA downstream of the poly(A) site"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7608167"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8417335"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7608167"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8417335"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9384595"}],"value":"Inhibited by nucleoside 3', 5'-bisphosphates"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7608167"}],"value":"Optimum pH is 8.0-8.8."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10805743"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12612077"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15565157"}],"value":"Interacts with RAI1 and RTT103"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q02792","intActId":"EBI-14845"},"interactantTwo":{"uniProtKBAccession":"P53063","geneName":"RAI1","intActId":"EBI-24206"},"numberOfExperiments":5,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12612077"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8417335"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 623 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7608167"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":1006,"modifier":"EXACT"}},"description":"5'-3' exoribonuclease 2","featureId":"PRO_0000071399"},{"type":"Repeat","location":{"start":{"value":955,"modifier":"EXACT"},"end":{"value":958,"modifier":"EXACT"}},"description":"1-1"},{"type":"Repeat","location":{"start":{"value":961,"modifier":"EXACT"},"end":{"value":964,"modifier":"EXACT"}},"description":"2-1"},{"type":"Repeat","location":{"start":{"value":972,"modifier":"EXACT"},"end":{"value":974,"modifier":"EXACT"}},"description":"2-2"},{"type":"Repeat","location":{"start":{"value":975,"modifier":"EXACT"},"end":{"value":978,"modifier":"EXACT"}},"description":"3-1"},{"type":"Repeat","location":{"start":{"value":984,"modifier":"EXACT"},"end":{"value":986,"modifier":"EXACT"}},"description":"3-2"},{"type":"Repeat","location":{"start":{"value":996,"modifier":"EXACT"},"end":{"value":999,"modifier":"EXACT"}},"description":"1-2"},{"type":"Region","location":{"start":{"value":492,"modifier":"EXACT"},"end":{"value":529,"modifier":"EXACT"}},"description":"Required for retention in the nucleus"},{"type":"Region","location":{"start":{"value":561,"modifier":"EXACT"},"end":{"value":584,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":939,"modifier":"EXACT"},"end":{"value":1006,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":955,"modifier":"EXACT"},"end":{"value":999,"modifier":"EXACT"}},"description":"2 X 4 AA repeats of S-R-Y-D, N-N-N-Y, Y-S-G-N"},{"type":"Coiled coil","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":544,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":561,"modifier":"EXACT"},"end":{"value":575,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":939,"modifier":"EXACT"},"end":{"value":956,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":959,"modifier":"EXACT"},"end":{"value":993,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":996,"modifier":"EXACT"},"end":{"value":1006,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":574,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Mutagenesis","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"Abrogates exonuclease activity and impairs termination of transcription by RNA polymerase II.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15565157"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":518,"modifier":"EXACT"},"end":{"value":518,"modifier":"EXACT"}},"description":"Causes mislocalization to the cytoplasm and suppresses the requirement for XRN1 function.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":520,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"Suppresses the requirement for XRN1 function.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["P"]}},{"type":"Mutagenesis","location":{"start":{"value":534,"modifier":"EXACT"},"end":{"value":534,"modifier":"EXACT"}},"description":"Causes mislocalization to the cytoplasm; when associated with A-535 and A-537.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":535,"modifier":"EXACT"},"end":{"value":535,"modifier":"EXACT"}},"description":"Causes mislocalization to the cytoplasm; when associated with A-534 and A-537.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":535,"modifier":"EXACT"},"end":{"value":535,"modifier":"EXACT"}},"description":"Causes mislocalization to the cytoplasm and suppresses the requirement for XRN1 function.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":537,"modifier":"EXACT"},"end":{"value":537,"modifier":"EXACT"}},"description":"Causes mislocalization to the cytoplasm; when associated with A-534 and A-535.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":537,"modifier":"EXACT"},"end":{"value":537,"modifier":"EXACT"}},"description":"Causes mislocalization to the cytoplasm and suppresses the requirement for XRN1 function.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9315672"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["E"]}},{"type":"Mutagenesis","location":{"start":{"value":683,"modifier":"EXACT"},"end":{"value":683,"modifier":"EXACT"}},"description":"In allele TAP1-1; activates transcription of the promoter-defective yeast SUP4 tRNA(Tyr) allele SUP4A53T61.","alternativeSequence":{"originalSequence":"Y","alternativeSequences":["H"]}},{"type":"Helix","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8JCH"}]},{"type":"Helix","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K5P"}]},{"type":"Helix","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":204,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":236,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K5P"}]},{"type":"Helix","location":{"start":{"value":284,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":316,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":336,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":344,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":361,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":362,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":366,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":591,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":594,"modifier":"EXACT"},"end":{"value":596,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":604,"modifier":"EXACT"},"end":{"value":613,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":617,"modifier":"EXACT"},"end":{"value":619,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9DSO"}]},{"type":"Helix","location":{"start":{"value":620,"modifier":"EXACT"},"end":{"value":644,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":658,"modifier":"EXACT"},"end":{"value":660,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":661,"modifier":"EXACT"},"end":{"value":663,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":677,"modifier":"EXACT"},"end":{"value":681,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":682,"modifier":"EXACT"},"end":{"value":687,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":692,"modifier":"EXACT"},"end":{"value":697,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":700,"modifier":"EXACT"},"end":{"value":707,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":708,"modifier":"EXACT"},"end":{"value":710,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K5P"}]},{"type":"Helix","location":{"start":{"value":714,"modifier":"EXACT"},"end":{"value":716,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":727,"modifier":"EXACT"},"end":{"value":729,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K5P"}]},{"type":"Beta strand","location":{"start":{"value":730,"modifier":"EXACT"},"end":{"value":732,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8JCH"}]},{"type":"Helix","location":{"start":{"value":738,"modifier":"EXACT"},"end":{"value":741,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":743,"modifier":"EXACT"},"end":{"value":751,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":754,"modifier":"EXACT"},"end":{"value":756,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":759,"modifier":"EXACT"},"end":{"value":762,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":763,"modifier":"EXACT"},"end":{"value":765,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K5P"}]},{"type":"Beta strand","location":{"start":{"value":771,"modifier":"EXACT"},"end":{"value":777,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":781,"modifier":"EXACT"},"end":{"value":788,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":789,"modifier":"EXACT"},"end":{"value":791,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9DSO"}]},{"type":"Beta strand","location":{"start":{"value":792,"modifier":"EXACT"},"end":{"value":795,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":800,"modifier":"EXACT"},"end":{"value":802,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Turn","location":{"start":{"value":804,"modifier":"EXACT"},"end":{"value":807,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":811,"modifier":"EXACT"},"end":{"value":815,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":829,"modifier":"EXACT"},"end":{"value":832,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":835,"modifier":"EXACT"},"end":{"value":837,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8K5P"}]},{"type":"Helix","location":{"start":{"value":839,"modifier":"EXACT"},"end":{"value":841,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":844,"modifier":"EXACT"},"end":{"value":849,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":850,"modifier":"EXACT"},"end":{"value":853,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":856,"modifier":"EXACT"},"end":{"value":858,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9DSO"}]},{"type":"Helix","location":{"start":{"value":865,"modifier":"EXACT"},"end":{"value":869,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8JCH"}]},{"type":"Helix","location":{"start":{"value":874,"modifier":"EXACT"},"end":{"value":881,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":885,"modifier":"EXACT"},"end":{"value":888,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8JCH"}]},{"type":"Helix","location":{"start":{"value":895,"modifier":"EXACT"},"end":{"value":898,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":899,"modifier":"EXACT"},"end":{"value":901,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Beta strand","location":{"start":{"value":918,"modifier":"EXACT"},"end":{"value":920,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]},{"type":"Helix","location":{"start":{"value":921,"modifier":"EXACT"},"end":{"value":930,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"9FMS"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0269","category":"Molecular function","name":"Exonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0698","category":"Biological process","name":"rRNA processing"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"id":"KW-0806","category":"Biological process","name":"Transcription termination"}],"references":[{"referenceNumber":1,"citation":{"id":"1628825","citationType":"journal article","authors":["Amberg D.C.","Goldstein A.L.","Cole C.N."],"citationCrossReferences":[{"database":"PubMed","id":"1628825"},{"database":"DOI","id":"10.1101/gad.6.7.1173"}],"title":"Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.","publicationDate":"1992","journal":"Genes Dev.","firstPage":"1173","lastPage":"1189","volume":"6"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"8417335","citationType":"journal article","authors":["Kenna M.","Stevens A.","McCammon M.","Douglas M.G."],"citationCrossReferences":[{"database":"PubMed","id":"8417335"},{"database":"DOI","id":"10.1128/mcb.13.1.341-350.1993"}],"title":"An essential yeast gene with homology to the exonuclease-encoding XRN1/KEM1 gene also encodes a protein with exoribonuclease activity.","publicationDate":"1993","journal":"Mol. Cell. Biol.","firstPage":"341","lastPage":"350","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","COFACTOR","SUBCELLULAR LOCATION"]},{"referenceNumber":3,"citation":{"id":"8497260","citationType":"journal article","authors":["Aldrich T.L.","di Segni G.","McConaughy B.L.","Keen N.J.","Whelen S.","Hall B.D."],"citationCrossReferences":[{"database":"PubMed","id":"8497260"},{"database":"DOI","id":"10.1128/mcb.13.6.3434-3444.1993"}],"title":"Structure of the yeast TAP1 protein: dependence of transcription activation on the DNA context of the target gene.","publicationDate":"1993","journal":"Mol. Cell. Biol.","firstPage":"3434","lastPage":"3444","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":4,"citation":{"id":"9169874","citationType":"journal article","authors":["Dujon B.","Albermann K.","Aldea M.","Alexandraki D.","Ansorge W.","Arino J.","Benes V.","Bohn C.","Bolotin-Fukuhara M.","Bordonne R.","Boyer J.","Camasses A.","Casamayor A.","Casas C.","Cheret G.","Cziepluch C.","Daignan-Fornier B.","Dang V.-D.","de Haan M.","Delius H.","Durand P.","Fairhead C.","Feldmann H.","Gaillon L.","Galisson F.","Gamo F.-J.","Gancedo C.","Goffeau A.","Goulding S.E.","Grivell L.A.","Habbig B.","Hand N.J.","Hani J.","Hattenhorst U.","Hebling U.","Hernando Y.","Herrero E.","Heumann K.","Hiesel R.","Hilger F.","Hofmann B.","Hollenberg C.P.","Hughes B.","Jauniaux J.-C.","Kalogeropoulos A.","Katsoulou C.","Kordes E.","Lafuente M.J.","Landt O.","Louis E.J.","Maarse A.C.","Madania A.","Mannhaupt G.","Marck C.","Martin R.P.","Mewes H.-W.","Michaux G.","Paces V.","Parle-McDermott A.G.","Pearson B.M.","Perrin A.","Pettersson B.","Poch O.","Pohl T.M.","Poirey R.","Portetelle D.","Pujol A.","Purnelle B.","Ramezani Rad M.","Rechmann S.","Schwager C.","Schweizer M.","Sor F.","Sterky F.","Tarassov I.A.","Teodoru C.","Tettelin H.","Thierry A.","Tobiasch E.","Tzermia M.","Uhlen M.","Unseld M.","Valens M.","Vandenbol M.","Vetter I.","Vlcek C.","Voet M.","Volckaert G.","Voss H.","Wambutt R.","Wedler H.","Wiemann S.","Winsor B.","Wolfe K.H.","Zollner A.","Zumstein E.","Kleine K."],"citationCrossReferences":[{"database":"PubMed","id":"9169874"},{"database":"DOI","id":"10.1038/387s098"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.","publicationDate":"1997","journal":"Nature","firstPage":"98","lastPage":"102","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"8497275","citationType":"journal article","authors":["Ganster R.W.","Shen W.","Schmidt M.C."],"citationCrossReferences":[{"database":"PubMed","id":"8497275"},{"database":"DOI","id":"10.1128/mcb.13.6.3650-3659.1993"}],"title":"Isolation of STD1, a high-copy-number suppressor of a dominant negative mutation in the yeast TATA-binding protein.","publicationDate":"1993","journal":"Mol. Cell. Biol.","firstPage":"3650","lastPage":"3659","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 929-1006"],"referenceComments":[{"value":"BJ1991","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"7608167","citationType":"journal article","authors":["Stevens A.","Poole T.L."],"citationCrossReferences":[{"database":"PubMed","id":"7608167"},{"database":"DOI","id":"10.1074/jbc.270.27.16063"}],"title":"5'-exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5'-exonuclease-1.","publicationDate":"1995","journal":"J. Biol. Chem.","firstPage":"16063","lastPage":"16069","volume":"270"},"referencePositions":["PROTEIN SEQUENCE OF 2-9","FUNCTION","COFACTOR","BIOPHYSICOCHEMICAL PROPERTIES"]},{"referenceNumber":8,"citation":{"id":"9207242","citationType":"journal article","authors":["Poole T.L.","Stevens A."],"citationCrossReferences":[{"database":"PubMed","id":"9207242"},{"database":"DOI","id":"10.1006/bbrc.1997.6877"}],"title":"Structural modifications of RNA influence the 5' exoribonucleolytic hydrolysis by XRN1 and HKE1 of Saccharomyces cerevisiae.","publicationDate":"1997","journal":"Biochem. Biophys. Res. Commun.","firstPage":"799","lastPage":"805","volume":"235"},"referencePositions":["FUNCTION"]},{"referenceNumber":9,"citation":{"id":"9384595","citationType":"journal article","authors":["Dichtl B.","Stevens A.","Tollervey D."],"citationCrossReferences":[{"database":"PubMed","id":"9384595"},{"database":"DOI","id":"10.1093/emboj/16.23.7184"}],"title":"Lithium toxicity in yeast is due to the inhibition of RNA processing enzymes.","publicationDate":"1997","journal":"EMBO J.","firstPage":"7184","lastPage":"7195","volume":"16"},"referencePositions":["FUNCTION","ACTIVITY REGULATION"]},{"referenceNumber":10,"citation":{"id":"9315672","citationType":"journal article","authors":["Johnson A.W."],"citationCrossReferences":[{"database":"PubMed","id":"9315672"},{"database":"DOI","id":"10.1128/mcb.17.10.6122"}],"title":"Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectively.","publicationDate":"1997","journal":"Mol. Cell. Biol.","firstPage":"6122","lastPage":"6130","volume":"17"},"referencePositions":["SUBCELLULAR LOCATION","MUTAGENESIS OF HIS-518; LEU-520; LYS-534; LYS-535 AND LYS-537"]},{"referenceNumber":11,"citation":{"id":"9488433","citationType":"journal article","authors":["Petfalski E.","Dandekar T.","Henry Y.","Tollervey D."],"citationCrossReferences":[{"database":"PubMed","id":"9488433"},{"database":"DOI","id":"10.1128/mcb.18.3.1181"}],"title":"Processing of the precursors to small nucleolar RNAs and rRNAs requires common components.","publicationDate":"1998","journal":"Mol. Cell. Biol.","firstPage":"1181","lastPage":"1189","volume":"18"},"referencePositions":["FUNCTION"]},{"referenceNumber":12,"citation":{"id":"9584178","citationType":"journal article","authors":["Villa T.","Ceradini F.","Presutti C.","Bozzoni I."],"citationCrossReferences":[{"database":"PubMed","id":"9584178"},{"database":"DOI","id":"10.1128/mcb.18.6.3376"}],"title":"Processing of the intron-encoded U18 small nucleolar RNA in the yeast Saccharomyces cerevisiae relies on both exo- and endonucleolytic activities.","publicationDate":"1998","journal":"Mol. Cell. Biol.","firstPage":"3376","lastPage":"3383","volume":"18"},"referencePositions":["FUNCTION"]},{"referenceNumber":13,"citation":{"id":"9891049","citationType":"journal article","authors":["Qu L.-H.","Henras A.","Lu Y.-J.","Zhou H.","Zhou W.-X.","Zhu Y.-Q.","Zhao J.","Henry Y.","Caizergues-Ferrer M.","Bachellerie J.-P."],"citationCrossReferences":[{"database":"PubMed","id":"9891049"},{"database":"DOI","id":"10.1128/mcb.19.2.1144"}],"title":"Seven novel methylation guide small nucleolar RNAs are processed from a common polycistronic transcript by Rat1p and RNase III in yeast.","publicationDate":"1999","journal":"Mol. Cell. Biol.","firstPage":"1144","lastPage":"1158","volume":"19"},"referencePositions":["FUNCTION"]},{"referenceNumber":14,"citation":{"id":"11030620","citationType":"journal article","authors":["Bousquet-Antonelli C.","Presutti C.","Tollervey D."],"citationCrossReferences":[{"database":"PubMed","id":"11030620"},{"database":"DOI","id":"10.1016/s0092-8674(00)00065-9"}],"title":"Identification of a regulated pathway for nuclear pre-mRNA turnover.","publicationDate":"2000","journal":"Cell","firstPage":"765","lastPage":"775","volume":"102"},"referencePositions":["FUNCTION"]},{"referenceNumber":15,"citation":{"id":"10805743","citationType":"journal article","authors":["Xue Y.","Bai X.","Lee I.","Kallstrom G.","Ho J.","Brown J.","Stevens A.","Johnson A.W."],"citationCrossReferences":[{"database":"PubMed","id":"10805743"},{"database":"DOI","id":"10.1128/mcb.20.11.4006-4015.2000"}],"title":"Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p.","publicationDate":"2000","journal":"Mol. Cell. Biol.","firstPage":"4006","lastPage":"4015","volume":"20"},"referencePositions":["FUNCTION","INTERACTION WITH RAI1"]},{"referenceNumber":16,"citation":{"id":"11142370","citationType":"journal article","authors":["Geerlings T.H.","Vos J.C.","Raue H.A."],"citationCrossReferences":[{"database":"PubMed","id":"11142370"},{"database":"DOI","id":"10.1017/s1355838200001540"}],"title":"The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5'->3' exonucleases.","publicationDate":"2000","journal":"RNA","firstPage":"1698","lastPage":"1703","volume":"6"},"referencePositions":["FUNCTION"]},{"referenceNumber":17,"citation":{"id":"11238889","citationType":"journal article","authors":["He F.","Jacobson A."],"citationCrossReferences":[{"database":"PubMed","id":"11238889"},{"database":"DOI","id":"10.1128/mcb.21.5.1515-1530.2001"}],"title":"Upf1p, Nmd2p, and Upf3p regulate the decapping and exonucleolytic degradation of both nonsense-containing mRNAs and wild-type mRNAs.","publicationDate":"2001","journal":"Mol. Cell. Biol.","firstPage":"1515","lastPage":"1530","volume":"21"},"referencePositions":["FUNCTION"]},{"referenceNumber":18,"citation":{"id":"12769851","citationType":"journal article","authors":["Danin-Kreiselman M.","Lee C.Y.","Chanfreau G."],"citationCrossReferences":[{"database":"PubMed","id":"12769851"},{"database":"DOI","id":"10.1016/s1097-2765(03)00137-0"}],"title":"RNase III-mediated degradation of unspliced pre-mRNAs and lariat introns.","publicationDate":"2003","journal":"Mol. Cell","firstPage":"1279","lastPage":"1289","volume":"11"},"referencePositions":["FUNCTION"]},{"referenceNumber":19,"citation":{"id":"12612077","citationType":"journal article","authors":["Sydorskyy Y.","Dilworth D.J.","Yi E.C.","Goodlett D.R.","Wozniak R.W.","Aitchison J.D."],"citationCrossReferences":[{"database":"PubMed","id":"12612077"},{"database":"DOI","id":"10.1128/mcb.23.6.2042-2054.2003"}],"title":"Intersection of the Kap123p-mediated nuclear import and ribosome export pathways.","publicationDate":"2003","journal":"Mol. Cell. Biol.","firstPage":"2042","lastPage":"2054","volume":"23"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY","INTERACTION WITH RAI1","SUBCELLULAR LOCATION"]},{"referenceNumber":20,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":21,"citation":{"id":"14561886","citationType":"journal article","authors":["Lee C.Y.","Lee A.","Chanfreau G."],"citationCrossReferences":[{"database":"PubMed","id":"14561886"},{"database":"DOI","id":"10.1261/rna.5126203"}],"title":"The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5'-end processing of S. cerevisiae box C/D snoRNAs.","publicationDate":"2003","journal":"RNA","firstPage":"1362","lastPage":"1370","volume":"9"},"referencePositions":["FUNCTION"]},{"referenceNumber":22,"citation":{"id":"15565157","citationType":"journal article","authors":["Kim M.","Krogan N.J.","Vasiljeva L.","Rando O.J.","Nedea E.","Greenblatt J.F.","Buratowski S."],"citationCrossReferences":[{"database":"PubMed","id":"15565157"},{"database":"DOI","id":"10.1038/nature03041"}],"title":"The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II.","publicationDate":"2004","journal":"Nature","firstPage":"517","lastPage":"522","volume":"432"},"referencePositions":["FUNCTION","IDENTIFICATION BY MASS SPECTROMETRY","IDENTIFICATION IN A COMPLEX WITH RTT103","MUTAGENESIS OF ASP-235"]},{"referenceNumber":23,"citation":{"id":"CI-DCFHRBLDEJPMN","citationType":"journal article","authors":["Kim M.","Krogan N.J.","Vasiljeva L.","Rando O.J.","Nedea E.","Greenblatt J.F.","Buratowski S."],"publicationDate":"2005","journal":"Nature","firstPage":"661","lastPage":"661","volume":"433"},"referencePositions":["ERRATUM OF PUBMED:15565157"]},{"referenceNumber":24,"citation":{"id":"16131592","citationType":"journal article","authors":["Fang F.","Phillips S.","Butler J.S."],"citationCrossReferences":[{"database":"PubMed","id":"16131592"},{"database":"DOI","id":"10.1261/rna.2900205"}],"title":"Rat1p and Rai1p function with the nuclear exosome in the processing and degradation of rRNA precursors.","publicationDate":"2005","journal":"RNA","firstPage":"1571","lastPage":"1578","volume":"11"},"referencePositions":["FUNCTION"]},{"referenceNumber":25,"citation":{"id":"16598041","citationType":"journal article","authors":["Luo W.","Johnson A.W.","Bentley D.L."],"citationCrossReferences":[{"database":"PubMed","id":"16598041"},{"database":"DOI","id":"10.1101/gad.1409106"}],"title":"The role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model.","publicationDate":"2006","journal":"Genes Dev.","firstPage":"954","lastPage":"965","volume":"20"},"referencePositions":["FUNCTION"]},{"referenceNumber":26,"citation":{"id":"19779198","citationType":"journal article","authors":["Holt L.J.","Tuch B.B.","Villen J.","Johnson A.D.","Gygi S.P.","Morgan D.O."],"citationCrossReferences":[{"database":"PubMed","id":"19779198"},{"database":"DOI","id":"10.1126/science.1172867"}],"title":"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.","publicationDate":"2009","journal":"Science","firstPage":"1682","lastPage":"1686","volume":"325"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-574","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M95626","properties":[{"key":"ProteinId","value":"AAA34960.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z11746","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"S61567","properties":[{"key":"ProteinId","value":"AAB26818.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z74956","properties":[{"key":"ProteinId","value":"CAA99240.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"L06011","properties":[{"key":"ProteinId","value":"AAA16950.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Unassigned_DNA"}]},{"database":"EMBL","id":"BK006948","properties":[{"key":"ProteinId","value":"DAA10830.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S20126","properties":[{"key":"EntryName","value":"S20126"}]},{"database":"RefSeq","id":"NP_014691.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001183467.1"}]},{"database":"PDB","id":"8JCH","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"M=1-1006"}]},{"database":"PDB","id":"8K5P","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"M=1-1006"}]},{"database":"PDB","id":"8Q6V","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.23 A"},{"key":"Chains","value":"B=1-1006"}]},{"database":"PDB","id":"9DSO","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.85 A"},{"key":"Chains","value":"A=1-981"}]},{"database":"PDB","id":"9FMS","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"2.65 A"},{"key":"Chains","value":"B=1-1006"}]},{"database":"PDBsum","id":"8JCH","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8K5P","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8Q6V","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9DSO","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9FMS","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-18199","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-36162","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-36908","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-47148","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-50566","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"34449","properties":[{"key":"Interactions","value":"640"}]},{"database":"ComplexPortal","id":"CPX-1332","properties":[{"key":"EntryName","value":"RAT1-RAI1 RNA polymerase II termination complex"}]},{"database":"DIP","id":"DIP-6692N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q02792","properties":[{"key":"Number of interactors","value":"1278"}]},{"database":"IntAct","id":"Q02792","properties":[{"key":"Interactions","value":"7"}]},{"database":"MINT","id":"Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YOR048C","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q02792","properties":[{"key":"glycosylation","value":"1 site"}]},{"database":"iPTMnet","id":"Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YOR048C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YOR048C_mRNA","properties":[{"key":"ProteinId","value":"YOR048C"},{"key":"GeneId","value":"YOR048C"}]},{"database":"GeneID","id":"854213","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YOR048C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000005574","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000005574","properties":[{"key":"GeneName","value":"RAT1"}]},{"database":"VEuPathDB","id":"FungiDB:YOR048C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2044","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_006038_1_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ITHDMVV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"28245at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-33592-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"3.1.13.B1","properties":[{"key":"OrganismId","value":"984"}]},{"database":"BioGRID-ORCS","id":"854213","properties":[{"key":"hits","value":"8 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:Q02792","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XV"}]},{"database":"RNAct","id":"Q02792","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0090730","properties":[{"key":"GoTerm","value":"C:Las1 complex"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"26638174"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14576278"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16823961"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9315672"}]},{"database":"GO","id":"GO:0110103","properties":[{"key":"GoTerm","value":"C:RNA polymerase II termination complex"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"25722373"}]},{"database":"GO","id":"GO:0004534","properties":[{"key":"GoTerm","value":"F:5'-3' RNA exonuclease activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8417335"}]},{"database":"GO","id":"GO:0003729","properties":[{"key":"GoTerm","value":"F:mRNA binding"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"23222640"}]},{"database":"GO","id":"GO:0019843","properties":[{"key":"GoTerm","value":"F:rRNA binding"},{"key":"GoEvidenceType","value":"IDA:GO_Central"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21811236"}]},{"database":"GO","id":"GO:0000448","properties":[{"key":"GoTerm","value":"P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26638174"}]},{"database":"GO","id":"GO:0000398","properties":[{"key":"GoTerm","value":"P:mRNA splicing, via spliceosome"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"33978753"}]},{"database":"GO","id":"GO:0110155","properties":[{"key":"GoTerm","value":"P:NAD-cap decapping"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"35173156"}]},{"database":"GO","id":"GO:0034244","properties":[{"key":"GoTerm","value":"P:negative regulation of transcription elongation by RNA polymerase II"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24501251"}]},{"database":"GO","id":"GO:0071028","properties":[{"key":"GoTerm","value":"P:nuclear mRNA surveillance"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"11030620"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27124216"}]},{"database":"GO","id":"GO:0071035","properties":[{"key":"GoTerm","value":"P:nuclear polyadenylation-dependent rRNA catabolic process"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"16131592"}]},{"database":"GO","id":"GO:1904595","properties":[{"key":"GoTerm","value":"P:positive regulation of termination of RNA polymerase II transcription"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25722373"}]},{"database":"GO","id":"GO:0006364","properties":[{"key":"GoTerm","value":"P:rRNA processing"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"11142370"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9488433"}]},{"database":"GO","id":"GO:0043144","properties":[{"key":"GoTerm","value":"P:sno(s)RNA processing"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"9488433"}]},{"database":"GO","id":"GO:0030847","properties":[{"key":"GoTerm","value":"P:termination of RNA polymerase II transcription, exosome-dependent"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"19818713"}]},{"database":"GO","id":"GO:0030846","properties":[{"key":"GoTerm","value":"P:termination of RNA polymerase II transcription, poly(A)-coupled"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"15565157"}]},{"database":"CDD","id":"cd18673","properties":[{"key":"EntryName","value":"PIN_XRN1-2-like"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.25.40.1050:FF:000003","properties":[{"key":"EntryName","value":"5'-3' exoribonuclease"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.12390:FF:000003","properties":[{"key":"EntryName","value":"5'-3' exoribonuclease"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.12390:FF:000005","properties":[{"key":"EntryName","value":"5'-3' exoribonuclease 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.1050","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.12390","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR027073","properties":[{"key":"EntryName","value":"5_3_exoribonuclease"}]},{"database":"InterPro","id":"IPR041412","properties":[{"key":"EntryName","value":"Xrn1_helical"}]},{"database":"InterPro","id":"IPR004859","properties":[{"key":"EntryName","value":"Xrn1_N"}]},{"database":"InterPro","id":"IPR017151","properties":[{"key":"EntryName","value":"Xrn2/3/4"}]},{"database":"PANTHER","id":"PTHR12341:SF41","properties":[{"key":"EntryName","value":"5'-3' EXORIBONUCLEASE 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12341","properties":[{"key":"EntryName","value":"5'->3' EXORIBONUCLEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17846","properties":[{"key":"EntryName","value":"XRN_M"},{"key":"MatchStatus","value":"2"}]},{"database":"Pfam","id":"PF03159","properties":[{"key":"EntryName","value":"XRN_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF037239","properties":[{"key":"EntryName","value":"Exonuclease_Xrn2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGVPSFFRWLSRKYPKIISPVLEEQPQIVDGVILPLDYSASNPNGELDNLYLDMNGIVHPCSHPENKPPPETEDEMLLAVFEYTNRVLNMARPRKVLVMAVDGVAPRAKMNQQRARRFRSARDAQIENEAREEIMRQREEVGEIIDDAVRNKKTWDSNAITPGTPFMDKLAAALRYWTAFKLATDPGWKNLQVIISDATVPGEGEHKIMNFIRSQRADPEYNPNTTHCIYGLDADLIFLGLATHEPHFKILREDVFAQDNRKRNNLKDTINMTEEEKQFLQKQNSEQPFLWLHINVLREYLSAELWVPGLPFTFDLERAIDDWVFMCFFCGNDFLPHLPCLDVRENSIDILLDIWKVVLPKLKTYMTCDGVLNLPSVETLLQHLGSREGDIFKTRHIQEARKKEAFERRKAQKNMSKGQDRHPTVATEQLQMYDTQGNLAKGSWNLTTSDMVRLKKELMLANEGNEEAIAKVKQQSDKNNELMKDISKEEIDDAVSKANKTNFNLAEVMKQKIINKKHRLEKDNEEEEIAKDSKKVKTEKAESECDLDAEIKDEIVADVNDRENSETTEVSRDSPVHSTVNVSEGPKNGVFDTDEFVKLFEPGYHERYYTAKFHVTPQDIEQLRKDMVKCYIEGVAWVLMYYYQGCASWNWFYPYHYAPLATDFHGFSHLEIKFEEGTPFLPYEQLMSVLPAASGHALPKIFRSLMSEPDSEIIDFYPEEFPIDMNGKKMSWQGIALLPFIDQDRLLTAVRAQYPLLSDAERARNIRGEPVLLISNKNANYERFSKKLYSKENNNNNVVVKFQHFKSGLSGIVSKDVEGFELNGKIVCPIQGGSLPNLSTTLILKMSYRLIPLPSRNKSIILNGFIPSEPVLTAYDLDSIMYKYNNQNYSRRWNFGNDLKQNIVPVGPKGITQYKPRTGGYRAFFYFAELSRNNVQPAHNYGRNSYNSQPGFNNSRYDGGNNNYRQNSNYRNNNYSGNRNSGQYSGNSYSRNNKQSRYDNSRANRR","length":1006,"molWeight":115934,"crc64":"5DDD5B0245F3E12A","md5":"2D99A2FE6D600D62A1061776098E613E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Repeat":6,"Region":4,"Coiled coil":2,"Compositional bias":4,"Modified residue":1,"Mutagenesis":9,"Helix":52,"Beta strand":25,"Turn":1},"uniParcId":"UPI0000052E3A"}}