{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q00531","uniProtkbId":"JI60_HORVU","entryAudit":{"firstPublicDate":"1992-12-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1992-12-01","entryVersion":92,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Hordeum vulgare","commonName":"Barley","taxonId":4513,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","Liliopsida","Poales","Poaceae","BOP clade","Pooideae","Triticodae","Triticeae","Hordeinae","Hordeum"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"60 kDa jasmonate-induced protein"},"ecNumbers":[{"value":"3.2.2.22"}]},"alternativeNames":[{"fullName":{"value":"rRNA N-glycosidase"}}]},"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.","ecNumber":"3.2.2.22"}},{"texts":[{"value":"By jasmonate"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":560,"modifier":"EXACT"}},"description":"60 kDa jasmonate-induced protein","featureId":"PRO_0000221400"},{"type":"Region","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]}],"keywords":[{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0611","category":"Biological process","name":"Plant defense"},{"id":"KW-0652","category":"Molecular function","name":"Protein synthesis inhibitor"},{"id":"KW-0800","category":"Molecular function","name":"Toxin"}],"references":[{"referenceNumber":1,"citation":{"id":"1511131","citationType":"journal article","authors":["Becker W.","Apel K."],"citationCrossReferences":[{"database":"PubMed","id":"1511131"},{"database":"DOI","id":"10.1007/bf00040538"}],"title":"Isolation and characterization of a cDNA clone encoding a novel jasmonate-induced protein of barley (Hordeum vulgare L.).","publicationDate":"1992","journal":"Plant Mol. Biol.","firstPage":"1065","lastPage":"1067","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"Leaf","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X66376","properties":[{"key":"ProteinId","value":"CAA47017.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"S25092","properties":[{"key":"EntryName","value":"S25092"}]},{"database":"AlphaFoldDB","id":"Q00531","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q00531","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0030598","properties":[{"key":"GoTerm","value":"F:rRNA N-glycosylase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0017148","properties":[{"key":"GoTerm","value":"P:negative regulation of translation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.420.10","properties":[{"key":"EntryName","value":"Ricin (A subunit), domain 1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR046533","properties":[{"key":"EntryName","value":"DUF6598"}]},{"database":"InterPro","id":"IPR036041","properties":[{"key":"EntryName","value":"Ribosome-inact_prot_sf"}]},{"database":"InterPro","id":"IPR001574","properties":[{"key":"EntryName","value":"Ribosome_inactivat_prot"}]},{"database":"InterPro","id":"IPR017988","properties":[{"key":"EntryName","value":"Ribosome_inactivat_prot_CS"}]},{"database":"InterPro","id":"IPR016138","properties":[{"key":"EntryName","value":"Ribosome_inactivat_prot_sub1"}]},{"database":"PANTHER","id":"PTHR33453","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33453:SF44","properties":[{"key":"EntryName","value":"RRNA N-GLYCOSYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20241","properties":[{"key":"EntryName","value":"DUF6598"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00161","properties":[{"key":"EntryName","value":"RIP"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56371","properties":[{"key":"EntryName","value":"Ribosome inactivating proteins (RIP)"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00275","properties":[{"key":"EntryName","value":"SHIGA_RICIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALDKVAPIVIVTPFNVMTDRYDEFIEKVRKALAGTAGAKVGPKPKSKVESPVLDKGTFPVEQPPRWIHVELHGKTQGTTTPKPKVAIRSDDAYIMGFTNSTGRWFQLSKTGTTYKLVDDKAVMAGFDGNYNTLVGGVNNLPTLNLNKFSMAQAAAALWNKASTLSGGIGSDVVDDDDGDMLRANDPVKQAVATLAVAVCEAARFSPVSKVVNAGWIKDKVSVTPDEVNYIKEWGDLSTALLSWMDKQYKDDATIFKKFNGIGITNGEEALAVVRLVKLVIRSNMAAAPTTDEQLLAYAQLPKHGRYMAEVFAVRIPATAGGDRPAAPSLCTAATAAATSFTARKKNTPRSKPAATARVTWSSLGHRLATSAYGPIVFNLDLHDGNCGQADEEEDEKNTGRIVCDAIGGDFSNYNKAISETVLTRCGPAEVIYAVLSNGVQGRVDVKLAGLQSRDEVVLVGRIVARSKLFDFGCVLFYNEAAGVRVRPGELVPLARHALAVPLHMPLTIELDIRHGGSGDEIVRGELEFKTAIDGLHTGRLVGVNDAEFEVTILWSEYPW","length":560,"molWeight":60362,"crc64":"7FD9BEAA024D4813","md5":"5FEB5C933B7904B5A7686B8B6676E3A7"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Active site":1},"uniParcId":"UPI000012DAB5"}}