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CRISPR clusters contain sequences complementary to antecedent mobile elements (spacers) and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities (Probable). This CRISPR-Cas system protects bacteria against transformation with plasmids containing DNA homologous to its spacer regions (PubMed:29979631)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"Processes pre-crRNA into individual crRNA units; accurate cleavage of pre-crRNA depends on a 3' stem-loop and the sequence of the bases in the stem. The mature crRNA is unusual for type III-A systems as it does not undergo 3' processing after Cas6 cleavage. Mature crRNA is about 71 nucleotides (nt long) with an 8 nt 5' handle and 28 nt repeat with a 10 nt stem-loop at the 3' end"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"a divalent metal cation","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60240"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"Ca(2+) and Mn(2+) stimulate processing of pre-crRNA."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"Pre-crRNA processing is inhibited by EDTA"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":8.395,"unit":"uM","substrate":"repeat pre-crRNA in presence of 0.125 mM CaCl(2)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"kcat is 12.156 min(-1) in presence of CaCl(2)."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"Deletion of this gene alone blocks maturation of pre-crRNA (PubMed:29979631). Deletion of the entire CRISPR-Cas locus (cas6 to cas2, Rv2824c to Rv2816c) decreases resistance to plasmids encoding spacer elements about 6-fold (PubMed:29979631)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29979631"}],"value":"Encoded in a type III-A CRISPR locus"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":314,"modifier":"EXACT"}},"description":"CRISPR-associated endoribonuclease Cas6","featureId":"PRO_0000418222"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Mutagenesis","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"Incorrect processing of pre-crRNA, binds pre-crRNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"Incorrect processing of pre-crRNA, no longer binds pre-crRNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"alternativeSequence":{"originalSequence":"GMG","alternativeSequences":["AMA"]}}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9634230","citationType":"journal article","authors":["Cole S.T.","Brosch R.","Parkhill J.","Garnier T.","Churcher C.M.","Harris D.E.","Gordon S.V.","Eiglmeier K.","Gas S.","Barry C.E. 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