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Cannot use NADH or NADPH instead of F420H(2) as the electron donor (PubMed:23240649)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16387854"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19039139"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22023140"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22244759"}],"value":"Is involved in the bioreductive activation of bicyclic 4-nitroimidazole prodrugs such as PA-824 and delamanid developed for anti-tuberculosis therapy against both replicating and persistent bacteria. It converts PA-824 into three primary metabolites resulting from reduction of the imidazole ring at C-3; the major one is the corresponding des-nitroimidazole that generates lethal reactive nitrogen species, including nitric oxide (NO), which appears to be responsible for the anaerobic killing activity. Ddn uses the reduced F420 produced by FGD1 to activate PA-824. 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Is not able to reduce the enantiomer (R)-PA-824 (the drug PA-824 is defined as the (S) isomer), and metronidazole (Mtz) (PubMed:22023140)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22023140"}],"value":"Optimum pH is 7.0-8.0."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16000731"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16000731"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the F420H(2)-dependent quinone reductase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"Deazaflavin-dependent 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