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Catalyzes the F420H(2)-dependent two-electron reduction of quinones to dihydroquinones, thereby preventing the formation of cytotoxic semiquinones obtained by the one-electron reduction pathway. In vitro, catalyzes the reduction of menadione to menadiol; since menaquinone is the sole quinone electron carrier in the respiratory chain in M.tuberculosis, the physiological electron acceptor for Fqr-mediated F420H(2) oxidation is therefore likely to be the endogenous menaquinone found in the membrane fraction of M.tuberculosis"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"oxidized coenzyme F420-(gamma-L-Glu)(n) + a quinol + H(+) = reduced coenzyme F420-(gamma-L-Glu)(n) + a quinone","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:39663"},{"database":"Rhea","id":"RHEA-COMP:12939"},{"database":"Rhea","id":"RHEA-COMP:14378"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:24646"},{"database":"ChEBI","id":"CHEBI:132124"},{"database":"ChEBI","id":"CHEBI:133980"},{"database":"ChEBI","id":"CHEBI:139511"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23240649"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":47.1,"unit":"uM","substrate":"menadione","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23240649"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23240649"}],"value":"kcat is 18.7 min(-1) for the reduction of menadione."}]}}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16000731"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the F420H(2)-dependent quinone reductase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"F420H(2)-dependent quinone reductase Rv1261c","featureId":"PRO_0000103778"},{"type":"Binding site","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:133980"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WP15"}],"ligand":{"name":"coenzyme F420-(gamma-Glu)n","id":"ChEBI:CHEBI:133980"}},{"type":"Binding site","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:133980"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WP15"}],"ligand":{"name":"coenzyme F420-(gamma-Glu)n","id":"ChEBI:CHEBI:133980"}},{"type":"Binding site","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:133980"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WP15"}],"ligand":{"name":"coenzyme F420-(gamma-Glu)n","id":"ChEBI:CHEBI:133980"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:133980"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WP15"}],"ligand":{"name":"coenzyme F420-(gamma-Glu)n","id":"ChEBI:CHEBI:133980"}},{"type":"Binding site","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:133980"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WP15"}],"ligand":{"name":"coenzyme F420-(gamma-Glu)n","id":"ChEBI:CHEBI:133980"}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9634230","citationType":"journal article","authors":["Cole S.T.","Brosch R.","Parkhill J.","Garnier T.","Churcher C.M.","Harris D.E.","Gordon S.V.","Eiglmeier K.","Gas S.","Barry C.E. 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