{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P9WM66","secondaryAccessions":["L0T4B3","P64685","Q10894"],"uniProtkbId":"INLPD_MYCTO","entryAudit":{"firstPublicDate":"2014-04-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2014-04-16","entryVersion":40,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)","taxonId":83331,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Mycobacteriales","Mycobacteriaceae","Mycobacterium","Mycobacterium tuberculosis complex"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}],"value":"(2E)-enoyl-[ACP] glycyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}],"value":"4.3.2.11"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}],"value":"(2E)-unsaturated fatty acyl-[ACP] glycyltransferase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WM67"}],"value":"Long-chain fatty acyl-CoA thioesterase FcoT"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WM67"}],"value":"3.1.2.-"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WM67"}],"value":"fcoT"},"orderedLocusNames":[{"value":"MT0107"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WM67"}],"value":"Involved in the biosynthesis of a unique class of isonitrile lipopeptides (INLPs) that seem to function as virulence factors in M.tuberculosis and to play a role in metal acquisition (By similarity). Catalyzes a Michael addition of glycine to the beta-position of an alpha,beta-unsaturated fatty acyl-[ACP], producing a (3R)-3-[(carboxymethyl)amino]fatty acid. Acts on the (2E)-decenoyl moiety loaded on the acyl-carrier protein (ACP) MT0109, forming the product (3R)-3-[(carboxymethyl)amino]decanoate released from the ACP (By similarity). Displays thioesterase activity with a preference for long chain fatty acyl groups (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a (3R)-3-[(carboxymethyl)amino]fatty acid + holo-[ACP] + H(+) = a (2E)-enoyl-[ACP] + glycine + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:74923"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"Rhea","id":"RHEA-COMP:9925"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57305"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78784"},{"database":"ChEBI","id":"CHEBI:193080"}],"ecNumber":"4.3.2.11","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:74925"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(3R)-3-[(carboxylmethyl)amino]decanoate + holo-[ACP] + H(+) = (2E)-decenoyl-[ACP] + glycine + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:75543"},{"database":"Rhea","id":"RHEA-COMP:9639"},{"database":"Rhea","id":"RHEA-COMP:9685"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57305"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:78467"},{"database":"ChEBI","id":"CHEBI:194341"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}]},"physiologicalReactions":[{"directionType":"right-to-left","reactionCrossReference":{"database":"Rhea","id":"RHEA:75545"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2HKM2"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a fatty acyl-CoA + H2O = a fatty acid + CoA + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16781"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:28868"},{"database":"ChEBI","id":"CHEBI:57287"},{"database":"ChEBI","id":"CHEBI:77636"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WM67"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the FcoT family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"(2E)-enoyl-[ACP] glycyltransferase","featureId":"PRO_0000427357"}],"keywords":[{"id":"KW-0276","category":"Biological process","name":"Fatty acid metabolism"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0443","category":"Biological process","name":"Lipid metabolism"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"12218036","citationType":"journal article","authors":["Fleischmann R.D.","Alland D.","Eisen J.A.","Carpenter L.","White O.","Peterson J.D.","DeBoy R.T.","Dodson R.J.","Gwinn M.L.","Haft D.H.","Hickey E.K.","Kolonay J.F.","Nelson W.C.","Umayam L.A.","Ermolaeva M.D.","Salzberg S.L.","Delcher A.","Utterback T.R.","Weidman J.F.","Khouri H.M.","Gill J.","Mikula A.","Bishai W.","Jacobs W.R. Jr.","Venter J.C.","Fraser C.M."],"citationCrossReferences":[{"database":"PubMed","id":"12218036"},{"database":"DOI","id":"10.1128/jb.184.19.5479-5490.2002"}],"title":"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.","publicationDate":"2002","journal":"J. Bacteriol.","firstPage":"5479","lastPage":"5490","volume":"184"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"CDC 1551 / Oshkosh","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE000516","properties":[{"key":"ProteinId","value":"AAK44329.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B70751","properties":[{"key":"EntryName","value":"B70751"}]},{"database":"RefSeq","id":"WP_003899810.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002755.2"}]},{"database":"AlphaFoldDB","id":"P9WM66","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P9WM66","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mtc:MT0107","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|83331.31.peg.112","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"HOGENOM","id":"CLU_124481_0_0_11","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001020","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0047617","properties":[{"key":"GoTerm","value":"F:fatty acyl-CoA hydrolase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"Gene3D","id":"3.10.129.30","properties":[{"key":"EntryName","value":"Rv0098, thioesterase-like hot dog domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR022598","properties":[{"key":"EntryName","value":"FcoT_ThioEstase"}]},{"database":"InterPro","id":"IPR043064","properties":[{"key":"EntryName","value":"FcoT_ThioEstase_Rv0098-like_sf"}]},{"database":"Pfam","id":"PF10862","properties":[{"key":"EntryName","value":"FcoT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSHTDLTPCTRVLASSGTVPIAEELLARVLEPYSCKGCRYLIDAQYSATEDSVLAYGNFTIGESAYIRSTGHFNAVELILCFNQLAYSAFAPAVLNEEIRVLRGWSIDDYCQHQLSSMLIRKASSRFRKPLNPQKFSARLLCRDLQVIERTWRYLKVPCVIEFWDENGGAASGEIELAALNIP","length":183,"molWeight":20529,"crc64":"EC4FCF0B8032355F","md5":"FC41867DA6AC2C47672CEA2A53782942"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":3,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0000139256"}}