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CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities (Probable). This CRISPR-Cas system protects bacteria against transformation with plasmids containing DNA homologous to its spacer regions (PubMed:29979631)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0A7HGA1"}],"value":"The subunit probably binds to the 5' handle of the crRNA, helping in discrimination between self- and non-self"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29979631"}],"value":"Deletion of the entire CRISPR-Cas locus (cas6 to cas2, Rv2824c to Rv2816c) decreases resistance to plasmids encoding spacer elements about 6-fold"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29979631"}],"value":"Encoded in a type III-A CRISPR locus"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CRISPR-associated Csm4 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"CRISPR system Cms protein Csm4","featureId":"PRO_0000418227"}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"9634230","citationType":"journal article","authors":["Cole S.T.","Brosch R.","Parkhill J.","Garnier T.","Churcher C.M.","Harris D.E.","Gordon S.V.","Eiglmeier K.","Gas S.","Barry C.E. 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