{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P96671","secondaryAccessions":["Q797H7"],"uniProtkbId":"YDEN_BACSU","entryAudit":{"firstPublicDate":"2009-01-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-05-01","entryVersion":117,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Putative hydrolase YdeN"},"ecNumbers":[{"value":"3.-.-.-"}]}},"genes":[{"geneName":{"value":"ydeN"},"orderedLocusNames":[{"value":"BSU05260"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the RBBP9 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"Putative hydrolase YdeN","featureId":"PRO_0000360180"},{"type":"Active site","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Active site","location":{"start":{"value":137,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Active site","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Beta strand","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Beta strand","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Beta strand","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Beta strand","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Beta strand","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Beta strand","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]},{"type":"Helix","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UXO"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-88S9H0MCUTQUV","citationType":"submission","authors":["Kasahara Y.","Nakai S.","Lee S.","Sadaie Y.","Ogasawara N."],"title":"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome.","publicationDate":"MAR-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"15159570","citationType":"journal article","authors":["Janda I.","Devedjiev Y.","Cooper D.","Chruszcz M.","Derewenda U.","Gabrys A.","Minor W.","Joachimiak A.","Derewenda Z.S."],"citationCrossReferences":[{"database":"PubMed","id":"15159570"},{"database":"DOI","id":"10.1107/s0907444904007188"}],"title":"Harvesting the high-hanging fruit: the structure of the YdeN gene product from Bacillus subtilis at 1.8 Angstroms resolution.","publicationDate":"2004","journal":"Acta Crystallogr. D","firstPage":"1101","lastPage":"1107","volume":"60"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)","PUTATIVE ACTIVE SITE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB001488","properties":[{"key":"ProteinId","value":"BAA19361.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB12333.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"H69778","properties":[{"key":"EntryName","value":"H69778"}]},{"database":"RefSeq","id":"NP_388407.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003234219.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"PDB","id":"1UXO","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.80 A"},{"key":"Chains","value":"A=2-190"}]},{"database":"PDBsum","id":"1UXO","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P96671","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P96671","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P96671","properties":[{"key":"Number of interactors","value":"51"}]},{"database":"STRING","id":"224308.BSU05260","properties":[{"key":"Description","value":"-"}]},{"database":"ESTHER","id":"bacsu-YDEN","properties":[{"key":"FamilyName","value":"Hydrolase_RBBP9_YdeN"}]},{"database":"PaxDb","id":"224308-BSU05260","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"939908","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB12333","properties":[{"key":"ProteinId","value":"CAB12333"},{"key":"GeneId","value":"BSU_05260"}]},{"database":"GeneID","id":"939908","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU05260","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.561","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG3545","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"P96671","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9804993at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P96671","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU05260-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P96671","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P96671","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0016787","properties":[{"key":"GoTerm","value":"F:hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR010662","properties":[{"key":"EntryName","value":"RBBP9/YdeN"}]},{"database":"PANTHER","id":"PTHR15394","properties":[{"key":"EntryName","value":"SERINE HYDROLASE RBBP9"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR15394:SF3","properties":[{"key":"EntryName","value":"SERINE HYDROLASE RBBP9"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06821","properties":[{"key":"EntryName","value":"Ser_hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTKQVYIIHGYRASSTNHWFPWLKKRLLADGVQADILNMPNPLQPRLEDWLDTLSLYQHTLHENTYLVAHSLGCPAILRFLEHLQLRKQLGGIILVSGFAKSLPTLQMLDEFTQGSFDHQKIIESAKHRAVIASKDDQIVPFSFSKDLAQQIDAALYEVQHGGHFLEDEGFTSLPIVYDVLTSYFSKETR","length":190,"molWeight":21722,"crc64":"B4D290FDDCD80362","md5":"CAC02EA0DD6303C87EFEED07C7009AF6"},"extraAttributes":{"countByCommentType":{"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":3,"Beta strand":7,"Helix":9},"uniParcId":"UPI00000330E6"}}