{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P80505","uniProtkbId":"G3P2_SYNY3","entryAudit":{"firstPublicDate":"1996-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":164,"sequenceVersion":3},"annotationScore":5.0,"organism":{"scientificName":"Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)","taxonId":1111708,"lineage":["Bacteria","Bacillati","Cyanobacteriota","Cyanophyceae","Synechococcales","Merismopediaceae","Synechocystis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9226260"}],"value":"Glyceraldehyde-3-phosphate dehydrogenase 2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}],"value":"1.2.1.59"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9226260"}],"value":"GAPDH 2"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9226260"}],"value":"NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9226260"}],"value":"NAD-dependent glyceraldehyde-3-phosphate dehydrogenase"}}]},"genes":[{"geneName":{"value":"gap2"},"orderedLocusNames":[{"value":"sll1342"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}],"value":"Involved in photosynthetic carbon assimilation. Catalyzes the NAD(P)-dependent oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG). The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG. It can use both NADP and NAD"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10296"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57604"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:59776"}],"ecNumber":"1.2.1.59","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:10300"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:57540"},{"database":"ChEBI","id":"CHEBI:57604"},{"database":"ChEBI","id":"CHEBI:57945"},{"database":"ChEBI","id":"CHEBI:59776"}],"ecNumber":"1.2.1.59","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glyceraldehyde-3-phosphate dehydrogenase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.4"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":337,"modifier":"EXACT"}},"description":"Glyceraldehyde-3-phosphate dehydrogenase 2","featureId":"PRO_0000145709"},{"type":"Active site","location":{"start":{"value":154,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}]},{"type":"Binding site","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Binding site","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":199,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59776"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"D-glyceraldehyde 3-phosphate","id":"ChEBI:CHEBI:59776"}},{"type":"Binding site","location":{"start":{"value":317,"modifier":"EXACT"},"end":{"value":317,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57540"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P00362"}],"ligand":{"name":"NAD(+)","id":"ChEBI:CHEBI:57540"}},{"type":"Site","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"Activates thiol group during catalysis","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q6GIL8"}]},{"type":"Sequence conflict","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"in Ref. 1; CAA58550","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["G"]}},{"type":"Sequence conflict","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"in Ref. 2; CAA60135/BAA18633","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"FG","alternativeSequences":["IA"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0324","category":"Biological process","name":"Glycolysis"},{"id":"KW-0520","category":"Ligand","name":"NAD"},{"id":"KW-0521","category":"Ligand","name":"NADP"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9226260","citationType":"journal article","authors":["Valverde F.","Losada M.","Serrano A."],"citationCrossReferences":[{"database":"PubMed","id":"9226260"},{"database":"DOI","id":"10.1128/jb.179.14.4513-4522.1997"}],"title":"Functional complementation of an Escherichia coli gap mutant supports an amphibolic role for NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase of Synechocystis sp. strain PCC 6803.","publicationDate":"1997","journal":"J. Bacteriol.","firstPage":"4513","lastPage":"4522","volume":"179"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","CATALYTIC ACTIVITY","SUBUNIT","SUBSTRATE SPECIFICITY"],"referenceComments":[{"value":"strain PCC 6803 / Kazusa","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"CI-8BFQ71ELBCS7R","citationType":"submission","authors":["Schubert M.","Brinkmann H.","Cerff R."],"publicationDate":"APR-1995","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"8905231","citationType":"journal article","authors":["Kaneko T.","Sato S.","Kotani H.","Tanaka A.","Asamizu E.","Nakamura Y.","Miyajima N.","Hirosawa M.","Sugiura M.","Sasamoto S.","Kimura T.","Hosouchi T.","Matsuno A.","Muraki A.","Nakazaki N.","Naruo K.","Okumura S.","Shimpo S.","Takeuchi C.","Wada T.","Watanabe A.","Yamada M.","Yasuda M.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"8905231"},{"database":"DOI","id":"10.1093/dnares/3.3.109"}],"title":"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.","publicationDate":"1996","journal":"DNA Res.","firstPage":"109","lastPage":"136","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 27184 / PCC 6803 / Kazusa","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-62UQACLAU7DSN","citationType":"submission","authors":["Valverde F.","Serrano A."],"publicationDate":"NOV-1995","submissionDatabase":"UniProtKB"},"referencePositions":["PROTEIN SEQUENCE OF 2-11"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X83564","properties":[{"key":"ProteinId","value":"CAA58550.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X86376","properties":[{"key":"ProteinId","value":"CAA60135.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BA000022","properties":[{"key":"ProteinId","value":"BAA18633.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S54141","properties":[{"key":"EntryName","value":"S54141"}]},{"database":"AlphaFoldDB","id":"P80505","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P80505","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P80505","properties":[{"key":"Number of interactors","value":"449"}]},{"database":"IntAct","id":"P80505","properties":[{"key":"Interactions","value":"3"}]},{"database":"STRING","id":"1148.gene:10500398","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"1148-gene:10500398","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"BAA18633","properties":[{"key":"ProteinId","value":"BAA18633"},{"key":"GeneId","value":"BAA18633"}]},{"database":"KEGG","id":"syn:sll1342","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0057","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"P80505","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P80505","properties":[{"key":"Description","value":"-"}]},{"database":"SABIO-RK","id":"P80505","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00109","properties":[{"key":"RectionId","value":"UER00184"}]},{"database":"Proteomes","id":"UP000001425","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0004365","properties":[{"key":"GoTerm","value":"F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}]},{"database":"GO","id":"GO:0047100","properties":[{"key":"GoTerm","value":"F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9226260"}]},{"database":"GO","id":"GO:0051287","properties":[{"key":"GoTerm","value":"F:NAD binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0050661","properties":[{"key":"GoTerm","value":"F:NADP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006006","properties":[{"key":"GoTerm","value":"P:glucose metabolic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006096","properties":[{"key":"GoTerm","value":"P:glycolytic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"CDD","id":"cd18126","properties":[{"key":"EntryName","value":"GAPDH_I_C"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05214","properties":[{"key":"EntryName","value":"GAPDH_I_N"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.360.10:FF:000002","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.720:FF:000001","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.360.10","properties":[{"key":"EntryName","value":"Dihydrodipicolinate Reductase, domain 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.720","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-like Domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR020831","properties":[{"key":"EntryName","value":"GlycerAld/Erythrose_P_DH"}]},{"database":"InterPro","id":"IPR020830","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_AS"}]},{"database":"InterPro","id":"IPR020829","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_cat"}]},{"database":"InterPro","id":"IPR020828","properties":[{"key":"EntryName","value":"GlycerAld_3-P_DH_NAD(P)-bd"}]},{"database":"InterPro","id":"IPR006424","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-P_DH_1"}]},{"database":"InterPro","id":"IPR036291","properties":[{"key":"EntryName","value":"NAD(P)-bd_dom_sf"}]},{"database":"NCBIfam","id":"TIGR01534","properties":[{"key":"EntryName","value":"GAPDH-I"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF005641","properties":[{"key":"EntryName","value":"PRK07403.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43148","properties":[{"key":"EntryName","value":"GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02800","properties":[{"key":"EntryName","value":"Gp_dh_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00044","properties":[{"key":"EntryName","value":"Gp_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF000149","properties":[{"key":"EntryName","value":"GAP_DH"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00078","properties":[{"key":"EntryName","value":"G3PDHDRGNASE"}]},{"database":"SMART","id":"SM00846","properties":[{"key":"EntryName","value":"Gp_dh_N"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55347","properties":[{"key":"EntryName","value":"Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51735","properties":[{"key":"EntryName","value":"NAD(P)-binding Rossmann-fold domains"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00071","properties":[{"key":"EntryName","value":"GAPDH"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTRVAINGFGRIGRNFLRCWLGRTDSQLEVVGINDTSDPRTNAHLLRYDSMLGKLDADISADENSITVNGKTIKCVSDRNPLNLPWAEWNVDLVIEATGVFVTHEGATKHVQAGAKKVLITAPGKGPNIGTYVVGVNAHEYKHEEYEVISNASCTTNCLAPFGKVINDNFGIIKGTMTTTHSYTGDQRILDASHRDLRRARAAAVNIVPTSTGAAKAVALVIPELQGKLNGIALRVPTPNVSVVDLVVQVEKNTIAEQVNGVLKEAANTSLKGVLEYTDLELVSSDFRGTDCSSTVDGSLTMVMGGDMVKVIAWYDNEWGYSQRVVDLAEIVAKNWK","length":337,"molWeight":36512,"crc64":"FC8FF0E7FEAC1585","md5":"C6B9CCF1B92C6A38E9945981B12AE24E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Active site":1,"Binding site":10,"Site":1,"Sequence conflict":2},"uniParcId":"UPI0000ED912B"}}