{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P77692","secondaryAccessions":["Q9XB43"],"uniProtkbId":"YKFI_ECOLI","entryAudit":{"firstPublicDate":"1998-07-15","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1997-02-01","entryVersion":135,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14594833"}],"value":"Toxin YkfI"}}},"genes":[{"geneName":{"value":"ykfI"},"orderedLocusNames":[{"value":"b0245"},{"value":"JW0234"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14594833"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"value":"Toxic component of a type IV toxin-antitoxin (TA) system (PubMed:14594833, PubMed:28257056, PubMed:28931012). Acts as a toxin inhibitor that blocks cell division and cell elongation via FtsZ and possibly also MreB (although no interaction with MreB has been proven) (PubMed:28931012). Overexpression results in inhibition of growth in liquid cultures and a decrease in colony formation (PubMed:14594833, PubMed:28257056, PubMed:28931012). These effects are overcome by concomitant expression of cognate antitoxin YafW, which leads to toxin loss by an unknown mechanism (PubMed:14594833, PubMed:28257056). Overexpression leads to formation of lemon-shaped cells and cell lysis; inactivated by overexpression of cognate antitoxin YafW but not when the 2 genes are coexpressed from the same plasmid (PubMed:28257056). Also neutralized by overexpression of non-cognate antitoxins YfjZ and CbeA (PubMed:28257056). Co-overexpression of toxin YkfI and antitoxin YafW leads to formation of elongated cells (PubMed:28257056)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"value":"Interacts with FtsZ but not MreB"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"}],"value":"Expressed in mid-log phase at lower levels than toxin relE"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"}],"value":"Deletion of 3 type IV toxin genes (cbtA, ykfI, ypfJ) leads to a slight reduction in resistance to oxidative stress, has no effect on cell growth"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Encoded in prophage CP4-6"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CbtA/YkfI/YpjF toxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Toxin YkfI","featureId":"PRO_0000168551"},{"type":"Mutagenesis","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Loss of interaction with FtsZ.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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