{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P77433","secondaryAccessions":["Q2MCB4"],"uniProtkbId":"YKGG_ECOLI","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2001-08-29","entryVersion":135,"sequenceVersion":2},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Uncharacterized protein YkgG"}}},"genes":[{"geneName":{"value":"ykgG"},"orderedLocusNames":[{"value":"b0308"},{"value":"JW5042"}]}],"comments":[{"texts":[{"value":"The E.coli ykgEFG operon is a clear homolog of the B.subtilis lutABC operon, however it is not able of restoring L-lactate utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG operon may contribute to lactate catabolism in E.coli, but under conditions other than those tested, or alternatively, the ykgEFG operon may be responsible for the catabolism of a metabolite other than (but perhaps related to) lactate in E.coli"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the LutC/YkgG family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAB18035.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":231,"modifier":"EXACT"}},"description":"Uncharacterized protein YkgG","featureId":"PRO_0000168570"}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"19201793","citationType":"journal article","authors":["Chai Y.","Kolter R.","Losick R."],"citationCrossReferences":[{"database":"PubMed","id":"19201793"},{"database":"DOI","id":"10.1128/jb.01464-08"}],"title":"A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation.","publicationDate":"2009","journal":"J. Bacteriol.","firstPage":"2423","lastPage":"2430","volume":"191"},"referencePositions":["LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON"],"referenceComments":[{"value":"K12","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U73857","properties":[{"key":"ProteinId","value":"AAB18035.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73411.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76092.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D64757","properties":[{"key":"EntryName","value":"D64757"}]},{"database":"RefSeq","id":"NP_414842.4","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000370307.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P77433","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P77433","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261523","properties":[{"key":"Interactions","value":"27"}]},{"database":"FunCoup","id":"P77433","properties":[{"key":"Number of interactors","value":"31"}]},{"database":"STRING","id":"511145.b0308","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P77433","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0308","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73411","properties":[{"key":"ProteinId","value":"AAC73411"},{"key":"GeneId","value":"b0308"}]},{"database":"GeneID","id":"945906","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW5042","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0308","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_01510","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_24685","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|511145.12.peg.316","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3354","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1556","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_090664_1_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P77433","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MPSCVNI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9794157at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P77433","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G6178-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P77433","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15911532"}]},{"database":"GO","id":"GO:0004459","properties":[{"key":"GoTerm","value":"F:L-lactate dehydrogenase (NAD+) activity"},{"key":"GoEvidenceType","value":"EXP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19196979"}]},{"database":"FunFam","id":"3.40.50.10420:FF:000006","properties":[{"key":"EntryName","value":"Lactate utilization protein C"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10420","properties":[{"key":"EntryName","value":"NagB/RpiA/CoA transferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024185","properties":[{"key":"EntryName","value":"FTHF_cligase-like_sf"}]},{"database":"InterPro","id":"IPR003741","properties":[{"key":"EntryName","value":"LUD_dom"}]},{"database":"InterPro","id":"IPR037171","properties":[{"key":"EntryName","value":"NagB/RpiA_transferase-like"}]},{"database":"PANTHER","id":"PTHR43682","properties":[{"key":"EntryName","value":"LACTATE UTILIZATION PROTEIN C"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43682:SF1","properties":[{"key":"EntryName","value":"LACTATE UTILIZATION PROTEIN C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02589","properties":[{"key":"EntryName","value":"LUD_dom"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF100950","properties":[{"key":"EntryName","value":"NagB/RpiA/CoA transferase-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQQRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISERLQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLPEYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVHGPVKAVYLIIEDC","length":231,"molWeight":25213,"crc64":"8CE0331F1FE1A6DD","md5":"5606B0AD845085D2901679303E1134DE"},"extraAttributes":{"countByCommentType":{"MISCELLANEOUS":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI000013B6EC"}}