{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P77425","secondaryAccessions":["Q2MBR2"],"uniProtkbId":"ALLC_ECOLI","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-02-01","entryVersion":178,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10601204"}],"value":"Allantoate amidohydrolase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20038185"}],"value":"AAH"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20038185"}],"value":"3.5.3.9"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17362992"}],"value":"Allantoate deiminase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10601204"}],"value":"allC"},"synonyms":[{"value":"glxB7"},{"value":"ylbB"}],"orderedLocusNames":[{"value":"b0516"},{"value":"JW0504"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10601204"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20038185"}],"value":"Involved in the anaerobic nitrogen utilization via the assimilation of allantoin (PubMed:10601204, PubMed:20038185). Catalyzes specifically the hydrolysis of allantoate to yield CO2, NH3 and S-ureidoglycine, which is unstable and readily undergoes a second deamination by S-ureidoglycine aminohydrolase AllE to yield S-ureidoglycolate and NH3 (PubMed:20038185). In vivo, the spontaneous release of S-ureidoglycolate and ammonia from S-ureidoglycine appears to be too slow to sustain an efficient flux of nitrogen (PubMed:20038185)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"allantoate + H2O + 2 H(+) = (S)-2-ureidoglycine + NH4(+) + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:27485"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:17536"},{"database":"ChEBI","id":"CHEBI:28938"},{"database":"ChEBI","id":"CHEBI:59947"}],"ecNumber":"3.5.3.9","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20038185"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25020232"}],"value":"Binds 2 Zn(2+) ions per subunit (PubMed:17362992). Also able to bind Mn(2+) (PubMed:25020232)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"17362992"}],"value":"Sulfate could be an allosteric effector of the enzyme that is responsible for stabilizing substrate binding. In addition, this anion effector may act as a counterion during enzyme-mediated catalysis"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"17362992"}],"value":"Optimum pH is 8. Less active under acidic conditions."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"10601204"}],"value":"Nitrogen metabolism; (S)-allantoin degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10601204"}],"value":"By glyoxylate and allantoin under anaerobic conditions"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase M20 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":411,"modifier":"EXACT"}},"description":"Allantoate amidohydrolase","featureId":"PRO_0000061957"},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25020232"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4PXD"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25020232"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4PXD"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1"}},{"type":"Binding site","location":{"start":{"value":215,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17536"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"}],"ligand":{"name":"allantoate","id":"ChEBI:CHEBI:17536"}},{"type":"Binding site","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":275,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17536"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25020232"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4PXD"}],"ligand":{"name":"allantoate","id":"ChEBI:CHEBI:17536"}},{"type":"Binding site","location":{"start":{"value":288,"modifier":"EXACT"},"end":{"value":288,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:17536"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25020232"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4PXD"}],"ligand":{"name":"allantoate","id":"ChEBI:CHEBI:17536"}},{"type":"Binding site","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":382,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17362992"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25020232"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"1Z2L"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"4PXD"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Mutagenesis","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"Loss of amidohydrolase activity. Large movement of the catalytic domain into a closed conformation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25020232"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Helix","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":18,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Turn","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":152,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1Z2L"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":191,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":193,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2IMO"}]},{"type":"Helix","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":221,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Turn","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":232,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":238,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":279,"modifier":"EXACT"},"end":{"value":291,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":316,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":332,"modifier":"EXACT"},"end":{"value":344,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":356,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":363,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Turn","location":{"start":{"value":364,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Beta strand","location":{"start":{"value":369,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":377,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]},{"type":"Helix","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":409,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4PXD"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0659","category":"Biological process","name":"Purine metabolism"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"10601204","citationType":"journal article","authors":["Cusa E.","Obradors N.","Baldoma L.","Badia J.","Aguilar J."],"citationCrossReferences":[{"database":"PubMed","id":"10601204"},{"database":"DOI","id":"10.1128/jb.181.24.7479-7484.1999"}],"title":"Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli.","publicationDate":"1999","journal":"J. Bacteriol.","firstPage":"7479","lastPage":"7484","volume":"181"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","PATHWAY","INDUCTION"],"referenceComments":[{"value":"K12 / ECL1","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"20038185","citationType":"journal article","authors":["Serventi F.","Ramazzina I.","Lamberto I.","Puggioni V.","Gatti R.","Percudani R."],"citationCrossReferences":[{"database":"PubMed","id":"20038185"},{"database":"DOI","id":"10.1021/cb900248n"}],"title":"Chemical basis of nitrogen recovery through the ureide pathway: formation and hydrolysis of S-ureidoglycine in plants and bacteria.","publicationDate":"2010","journal":"ACS Chem. Biol.","firstPage":"203","lastPage":"214","volume":"5"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY"]},{"referenceNumber":6,"citation":{"id":"17362992","citationType":"journal article","authors":["Agarwal R.","Burley S.K.","Swaminathan S."],"citationCrossReferences":[{"database":"PubMed","id":"17362992"},{"database":"DOI","id":"10.1016/j.jmb.2007.02.028"}],"title":"Structural analysis of a ternary complex of allantoate amidohydrolase from Escherichia coli reveals its mechanics.","publicationDate":"2007","journal":"J. Mol. Biol.","firstPage":"450","lastPage":"463","volume":"368"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 2-411 IN COMPLEX WITH 2 ZINC IONS AND ALLANTOATE","COFACTOR","ACTIVITY REGULATION","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT"]},{"referenceNumber":7,"citation":{"id":"25020232","citationType":"journal article","authors":["Shin I.","Han K.","Rhee S."],"citationCrossReferences":[{"database":"PubMed","id":"25020232"},{"database":"DOI","id":"10.1016/j.jmb.2014.06.017"}],"title":"Structural insights into the substrate specificity of (s)-ureidoglycolate amidohydrolase and its comparison with allantoate amidohydrolase.","publicationDate":"2014","journal":"J. Mol. Biol.","firstPage":"3028","lastPage":"3040","volume":"426"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF MUTANT ALA-126 IN COMPLEX WITH MANGANESE ION AND ALLANTOATE","MUTAGENESIS OF GLU-126","COFACTOR"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U89279","properties":[{"key":"ProteinId","value":"AAB93857.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U82664","properties":[{"key":"ProteinId","value":"AAB40268.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73618.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76294.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"C64783","properties":[{"key":"EntryName","value":"C64783"}]},{"database":"RefSeq","id":"NP_415049.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001310618.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"1Z2L","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.25 A"},{"key":"Chains","value":"A/B=2-411"}]},{"database":"PDB","id":"2IMO","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.80 A"},{"key":"Chains","value":"A/B=2-411"}]},{"database":"PDB","id":"4PXD","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A/B=1-411"}]},{"database":"PDBsum","id":"1Z2L","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2IMO","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4PXD","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P77425","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P77425","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261167","properties":[{"key":"Interactions","value":"67"}]},{"database":"BioGRID","id":"849538","properties":[{"key":"Interactions","value":"1"}]},{"database":"DIP","id":"DIP-9088N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P77425","properties":[{"key":"Number of interactors","value":"397"}]},{"database":"IntAct","id":"P77425","properties":[{"key":"Interactions","value":"9"}]},{"database":"STRING","id":"511145.b0516","properties":[{"key":"Description","value":"-"}]},{"database":"DrugBank","id":"DB04380","properties":[{"key":"GenericName","value":"Allantoate"}]},{"database":"MEROPS","id":"M20.976","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0516","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"945150","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73618","properties":[{"key":"ProteinId","value":"AAC73618"},{"key":"GeneId","value":"b0516"}]},{"database":"GeneID","id":"945150","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW0504","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0516","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_02530","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.1762","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3388","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0624","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_024588_6_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P77425","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"IWPHGRW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9808195at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P77425","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G6285-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:G6285-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"3.5.3.4","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"BRENDA","id":"3.5.3.9","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"UniPathway","id":"UPA00395","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"P77425","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P77425","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P77425","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0047652","properties":[{"key":"GoTerm","value":"F:allantoate deiminase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10601204"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20038185"}]},{"database":"GO","id":"GO:0030145","properties":[{"key":"GoTerm","value":"F:manganese ion binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20038185"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17362992"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17362992"}]},{"database":"GO","id":"GO:0009442","properties":[{"key":"GoTerm","value":"P:allantoin assimilation pathway"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"10601204"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19935661"}]},{"database":"CDD","id":"cd03884","properties":[{"key":"EntryName","value":"M20_bAS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.360:FF:000013","properties":[{"key":"EntryName","value":"Allantoate amidohydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.630.10:FF:000044","properties":[{"key":"EntryName","value":"Allantoate amidohydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.360","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.630.10","properties":[{"key":"EntryName","value":"Zn peptidases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017591","properties":[{"key":"EntryName","value":"Allantoate_amidohydrolase"}]},{"database":"InterPro","id":"IPR036264","properties":[{"key":"EntryName","value":"Bact_exopeptidase_dim_dom"}]},{"database":"InterPro","id":"IPR010158","properties":[{"key":"EntryName","value":"HyuC"}]},{"database":"InterPro","id":"IPR002933","properties":[{"key":"EntryName","value":"Peptidase_M20"}]},{"database":"NCBIfam","id":"TIGR03176","properties":[{"key":"EntryName","value":"AllC"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01879","properties":[{"key":"EntryName","value":"hydantase"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006768","properties":[{"key":"EntryName","value":"PRK09290.1-1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF006771","properties":[{"key":"EntryName","value":"PRK09290.1-5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32494:SF5","properties":[{"key":"EntryName","value":"ALLANTOATE AMIDOHYDROLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32494","properties":[{"key":"EntryName","value":"ALLANTOATE DEIMINASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01546","properties":[{"key":"EntryName","value":"Peptidase_M20"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF001235","properties":[{"key":"EntryName","value":"Amidase_carbamoylase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55031","properties":[{"key":"EntryName","value":"Bacterial exopeptidase dimerisation domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53187","properties":[{"key":"EntryName","value":"Zn-dependent exopeptidases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFKKRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLAIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSFVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVTLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNVVPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELVATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDLAEGVKTLALMLYQLAWQK","length":411,"molWeight":45694,"crc64":"C55D1EF854A8F513","md5":"EA0D6BC313BF389E37EAEE4A2E55143A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":9,"Mutagenesis":1,"Helix":16,"Beta strand":17,"Turn":3},"uniParcId":"UPI000012589D"}}