{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P76268","secondaryAccessions":["O07975"],"uniProtkbId":"KDGR_ECOLI","entryAudit":{"firstPublicDate":"1998-07-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-02-01","entryVersion":176,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"HTH-type transcriptional regulator KdgR"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"4359651"}],"value":"kdgR"},"synonyms":[{"value":"yebP"}],"orderedLocusNames":[{"value":"b1827"},{"value":"JW1816"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15659677"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"4359651"}],"value":"Transcriptional repressor that negatively regulates the expression of kdgT, kdgK and kdgA, which encode proteins involved in transport and catabolism of 2-keto-3-deoxygluconate (KDG) (PubMed:4359651). Also represses expression of eda, which encodes the Entner-Doudoroff aldolase, by binding to its P2 promoter region (PubMed:15659677)"}],"commentType":"FUNCTION"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P76268","intActId":"EBI-1123676"},"interactantTwo":{"uniProtKBAccession":"P64503","geneName":"yebV","intActId":"EBI-9126792"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cytoplasm","id":"SL-0086"}}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":263,"modifier":"EXACT"}},"description":"HTH-type transcriptional regulator KdgR","featureId":"PRO_0000201760"},{"type":"Domain","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"HTH iclR-type","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00393"}]},{"type":"Domain","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"IclR-ED","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00394"}]},{"type":"DNA binding","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"H-T-H motif","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00393"}]},{"type":"Helix","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Beta strand","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Beta strand","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Beta strand","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Helix","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":153,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Helix","location":{"start":{"value":157,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Helix","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Beta strand","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Beta strand","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Beta strand","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]},{"type":"Helix","location":{"start":{"value":235,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1YSP"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"9097040","citationType":"journal article","authors":["Itoh T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Kasai H.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097040"},{"database":"DOI","id":"10.1093/dnares/3.6.379"}],"title":"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"379","lastPage":"392","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"4359651","citationType":"journal article","authors":["Pouyssegur J.","Stoeber F."],"citationCrossReferences":[{"database":"PubMed","id":"4359651"},{"database":"DOI","id":"10.1128/jb.117.2.641-651.1974"}],"title":"Genetic control of the 2-keto-3-deoxy-d-gluconate metabolism in Escherichia coli K-12: kdg regulon.","publicationDate":"1974","journal":"J. Bacteriol.","firstPage":"641","lastPage":"651","volume":"117"},"referencePositions":["FUNCTION AS A REPRESSOR"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"15659677","citationType":"journal article","authors":["Murray E.L.","Conway T."],"citationCrossReferences":[{"database":"PubMed","id":"15659677"},{"database":"DOI","id":"10.1128/jb.187.3.991-1000.2005"}],"title":"Multiple regulators control expression of the Entner-Doudoroff aldolase (Eda) of Escherichia coli.","publicationDate":"2005","journal":"J. Bacteriol.","firstPage":"991","lastPage":"1000","volume":"187"},"referencePositions":["FUNCTION","DNA-BINDING"]},{"referenceNumber":6,"citation":{"id":"CI-69CROPFQEA5O0","citationType":"submission","authoringGroup":["Midwest center for structural genomics (MCSG)"],"title":"Structural study of effector binding specificity in iclR transcriptional regulators.","publicationDate":"MAR-2005","submissionDatabase":"PDB data bank"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 88-263"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"1YSP"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74897.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA15635.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"C64944","properties":[{"key":"EntryName","value":"C64944"}]},{"database":"RefSeq","id":"NP_416341.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001262203.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"1YSP","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.80 A"},{"key":"Chains","value":"A=88-263"}]},{"database":"PDBsum","id":"1YSP","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261734","properties":[{"key":"Interactions","value":"149"}]},{"database":"BioGRID","id":"850489","properties":[{"key":"Interactions","value":"1"}]},{"database":"DIP","id":"DIP-10057N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P76268","properties":[{"key":"Number of interactors","value":"188"}]},{"database":"IntAct","id":"P76268","properties":[{"key":"Interactions","value":"4"}]},{"database":"STRING","id":"511145.b1827","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b1827","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74897","properties":[{"key":"ProteinId","value":"AAC74897"},{"key":"GeneId","value":"b1827"}]},{"database":"GeneID","id":"946129","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW1816","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1827","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_10415","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.423","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3772","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1414","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_062618_6_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"RHSMRMF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9807558at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G7003-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P76268","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P76268","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0003700","properties":[{"key":"GoTerm","value":"F:DNA-binding transcription factor activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15659677"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"4359651"}]},{"database":"GO","id":"GO:0045892","properties":[{"key":"GoTerm","value":"P:negative regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15659677"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"4359651"}]},{"database":"CDD","id":"cd00090","properties":[{"key":"EntryName","value":"HTH_ARSR"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.10.10:FF:000109","properties":[{"key":"EntryName","value":"DNA-binding transcriptional regulator KdgR"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.450.40:FF:000009","properties":[{"key":"EntryName","value":"DNA-binding transcriptional regulator KdgR"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.450.40","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.10.10","properties":[{"key":"EntryName","value":"Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR011991","properties":[{"key":"EntryName","value":"ArsR-like_HTH"}]},{"database":"InterPro","id":"IPR029016","properties":[{"key":"EntryName","value":"GAF-like_dom_sf"}]},{"database":"InterPro","id":"IPR050707","properties":[{"key":"EntryName","value":"HTH_MetabolicPath_Reg"}]},{"database":"InterPro","id":"IPR014757","properties":[{"key":"EntryName","value":"Tscrpt_reg_IclR_C"}]},{"database":"InterPro","id":"IPR005471","properties":[{"key":"EntryName","value":"Tscrpt_reg_IclR_N"}]},{"database":"InterPro","id":"IPR036388","properties":[{"key":"EntryName","value":"WH-like_DNA-bd_sf"}]},{"database":"InterPro","id":"IPR036390","properties":[{"key":"EntryName","value":"WH_DNA-bd_sf"}]},{"database":"NCBIfam","id":"NF011671","properties":[{"key":"EntryName","value":"PRK15090.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30136","properties":[{"key":"EntryName","value":"HELIX-TURN-HELIX TRANSCRIPTIONAL REGULATOR, ICLR FAMILY"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30136:SF7","properties":[{"key":"EntryName","value":"HTH-TYPE TRANSCRIPTIONAL REGULATOR KDGR-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09339","properties":[{"key":"EntryName","value":"HTH_IclR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01614","properties":[{"key":"EntryName","value":"IclR_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00346","properties":[{"key":"EntryName","value":"HTH_ICLR"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55781","properties":[{"key":"EntryName","value":"GAF domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46785","properties":[{"key":"EntryName","value":"Winged helix' DNA-binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51077","properties":[{"key":"EntryName","value":"HTH_ICLR"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51078","properties":[{"key":"EntryName","value":"ICLR_ED"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMSKSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMRELSRLTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEVKQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFGVVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF","length":263,"molWeight":30029,"crc64":"5744F66467922C72","md5":"0FF36B3ABA1FF928E34CE9E69083CF4F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1},"countByFeatureType":{"Chain":1,"Domain":2,"DNA binding":1,"Helix":5,"Beta strand":6},"uniParcId":"UPI000012DD31"}}