{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P76064","secondaryAccessions":["Q2MBE3"],"uniProtkbId":"YDAT_ECOLI","entryAudit":{"firstPublicDate":"1998-07-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-02-01","entryVersion":131,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Transcriptional regulator YdaT"}}},"genes":[{"geneName":{"value":"ydaT"},"orderedLocusNames":[{"value":"b1358"},{"value":"JW1353"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205229"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38153127"}],"value":"Transcriptional regulator that causes a severe detrimental growth effect and reduces cell viability (PubMed:38153127). When expressed, it alters expression of a variety of bacterial regulons normally controlled by the transcriptional regulatory protein RcsA, resulting in deficient lipopolysaccharide biosynthesis and cell division (PubMed:38153127). YdaT has no effect on Rac prophage excision (PubMed:38153127). Overexpression of ydaST reduces growth and leads to loss of cell viability (PubMed:29205228). May contribute to toxicity and morphological defects (PubMed:29205229)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205229"}],"value":"Not expressed under normal physiological conditions (PubMed:29205228). Expression is repressed by the transcriptional repressor RacR (PubMed:29205228, PubMed:29205229)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205229"}],"value":"The toxic effects of racR silencing can be observed in the ydaT deletion mutant (where YdaS levels are elevated), but the ydaS-ydaT double deletion mutant shows complete attenuation of the toxic effects of racR silencing"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205229"}],"value":"Encoded in the Rac prophage region"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19943910"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205228"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29205229"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29695625"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29695627"}],"value":"The ydaST locus was predicted to encode a toxin/antitoxin (TA) pair in a genome-wide prediction of TA pairs, however it was shown in subsequent studies that it does not form a toxin/antitoxin pair (PubMed:19943910, PubMed:29205228, PubMed:29205229). Whether or not the YdaST-RacR module forms a toxin-repressor combination or an atypical toxin-antitoxin system, or whether they are actually essential regulatory components of the prophage genome, has been debated (PubMed:29695625, PubMed:29695627)"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Transcriptional regulator YdaT","featureId":"PRO_0000168910"}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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