{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P76046","secondaryAccessions":["P77411"],"uniProtkbId":"YCJX_ECOLI","entryAudit":{"firstPublicDate":"1998-07-15","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1997-02-01","entryVersion":143,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Ras-like GTPase YcjX"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"value":"3.6.5.2"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Stress protein YcjX"}}]},"genes":[{"geneName":{"value":"ycjX"},"orderedLocusNames":[{"value":"b1321"},{"value":"JW1314"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"value":"Binds GTP and GDP. Has intrinsic GTPase activity. Does not hydrolyze ATP. May act as a transducer of stress responses"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + H2O = GDP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19669"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58189"}],"ecNumber":"3.6.5.2","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"value":"Alternates between an inactive form bound to GDP and an active form bound to GTP. Likely activated by a guanine nucleotide-exchange factor (GEF)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"value":"Monomer in solution"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"To H.influenzae HI_1637"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"Ras-like GTPase YcjX","featureId":"PRO_0000168896"},{"type":"Motif","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"Walker A motif","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}]},{"type":"Binding site","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":338,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":338,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":340,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":340,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":341,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GDP","id":"ChEBI:CHEBI:58189"}},{"type":"Binding site","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8EG04"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Modified residue","location":{"start":{"value":249,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18723842"}]}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9097039","citationType":"journal article","authors":["Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kasai H.","Kashimoto K.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Takeuchi Y.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097039"},{"database":"DOI","id":"10.1093/dnares/3.6.363"}],"title":"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"363","lastPage":"377","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]","SEQUENCE REVISION"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"18723842","citationType":"journal article","authors":["Zhang J.","Sprung R.","Pei J.","Tan X.","Kim S.","Zhu H.","Liu C.F.","Grishin N.V.","Zhao Y."],"citationCrossReferences":[{"database":"PubMed","id":"18723842"},{"database":"DOI","id":"10.1074/mcp.m800187-mcp200"}],"title":"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.","publicationDate":"2009","journal":"Mol. Cell. Proteomics","firstPage":"215","lastPage":"225","volume":"8"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-249","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"K12 / JW1106","type":"STRAIN"},{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74403.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA14902.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D64881","properties":[{"key":"EntryName","value":"D64881"}]},{"database":"RefSeq","id":"NP_415837.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000825775.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4260148","properties":[{"key":"Interactions","value":"13"}]},{"database":"DIP","id":"DIP-11615N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P76046","properties":[{"key":"Number of interactors","value":"30"}]},{"database":"IntAct","id":"P76046","properties":[{"key":"Interactions","value":"3"}]},{"database":"STRING","id":"511145.b1321","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b1321","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74403","properties":[{"key":"ProteinId","value":"AAC74403"},{"key":"GeneId","value":"b1321"}]},{"database":"GeneID","id":"945872","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW1314","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1321","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_07735","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|511145.12.peg.1378","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3680","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG3106","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_043657_0_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FDYPGEW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9777645at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G6659-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P76046","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P76046","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0003925","properties":[{"key":"GoTerm","value":"F:G protein activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0019003","properties":[{"key":"GoTerm","value":"F:GDP binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"InterPro","id":"IPR007413","properties":[{"key":"EntryName","value":"YcjX-like"}]},{"database":"PANTHER","id":"PTHR38605:SF1","properties":[{"key":"EntryName","value":"ATPASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR38605","properties":[{"key":"EntryName","value":"ATPASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04317","properties":[{"key":"EntryName","value":"DUF463"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF019381","properties":[{"key":"EntryName","value":"YcjX"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLLNIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGVSEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLLNGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKEQGLHFIQPGRFVLPGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHFLRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLFAATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNGEKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHIRLDAALEFLIGDKLR","length":465,"molWeight":52609,"crc64":"5FF02E4F7FA1C10C","md5":"D7417224C85440CCBD03F55745BC8C2F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Motif":1,"Binding site":22,"Modified residue":1},"uniParcId":"UPI000013A6E7"}}