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Not induced by DNA damaging agents (PubMed:32409837)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32409837"}],"value":"Deletion of the gene slightly increases cellular sensitivity to the cross-linking agent mechlorethamine (PubMed:32409837). It does not affect sensitivity to the methylating agent methylmethanesulfonate (MMS) (PubMed:32409837)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DNA glycosylase AlkZ-like family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":410,"modifier":"EXACT"}},"description":"Interstrand DNA cross-link repair glycosylase","featureId":"PRO_0000168761"},{"type":"Motif","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"QXD; important for activity","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32409837"}]},{"type":"Mutagenesis","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"Abrogates d7mG excision.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32409837"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"Abrogates d7mG excision.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32409837"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"No effect on base excision activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32409837"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}}],"keywords":[{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"8905232","citationType":"journal article","authors":["Oshima T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Honjo A.","Ikemoto K.","Inada T.","Itoh T.","Kajihara M.","Kanai K.","Kashimoto K.","Kimura S.","Kitagawa M.","Makino K.","Masuda S.","Miki T.","Mizobuchi K.","Mori H.","Motomura K.","Nakamura Y.","Nashimoto H.","Nishio Y.","Saito N.","Sampei G.","Seki Y.","Tagami H.","Takemoto K.","Wada C.","Yamamoto Y.","Yano M.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"8905232"},{"database":"DOI","id":"10.1093/dnares/3.3.137"}],"title":"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"137","lastPage":"155","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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