{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P75045","uniProtkbId":"RNM5_MYCPN","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-02-01","entryVersion":115,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Mycoplasmoides pneumoniae (strain ATCC 29342 / M129 / Subtype 1)","commonName":"Mycoplasma pneumoniae","taxonId":272634,"lineage":["Bacteria","Bacillati","Mycoplasmatota","Mycoplasmoidales","Mycoplasmoidaceae","Mycoplasmoides"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"Ribonuclease M5"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"3.1.26.8"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"RNase M5"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"Ribosomal RNA terminal maturase M5"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"rnmV"},"orderedLocusNames":[{"value":"MPN_072"}],"orfNames":[{"value":"D09_orf178"},{"value":"MP083"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor.","ecNumber":"3.1.26.8","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"Binds two Mg(2+) per subunit."}]}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"value":"Belongs to the ribonuclease M5 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"Ribonuclease M5","featureId":"PRO_0000210402"},{"type":"Domain","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"Toprim","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}]},{"type":"Binding site","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01469"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0690","category":"Biological process","name":"Ribosome biogenesis"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0698","category":"Biological process","name":"rRNA processing"},{"id":"KW-0699","category":"Molecular function","name":"rRNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"8948633","citationType":"journal article","authors":["Himmelreich R.","Hilbert H.","Plagens H.","Pirkl E.","Li B.-C.","Herrmann R."],"citationCrossReferences":[{"database":"PubMed","id":"8948633"},{"database":"DOI","id":"10.1093/nar/24.22.4420"}],"title":"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae.","publicationDate":"1996","journal":"Nucleic Acids Res.","firstPage":"4420","lastPage":"4449","volume":"24"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 29342 / M129 / Subtype 1","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00089","properties":[{"key":"ProteinId","value":"AAB95731.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S73409","properties":[{"key":"EntryName","value":"S73409"}]},{"database":"RefSeq","id":"WP_010874429.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000912.1"}]},{"database":"AlphaFoldDB","id":"P75045","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P75045","properties":[{"key":"Description","value":"-"}]},{"database":"IntAct","id":"P75045","properties":[{"key":"Interactions","value":"3"}]},{"database":"STRING","id":"272634.MPN_072","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"272634-MPN_072","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAB95731","properties":[{"key":"ProteinId","value":"AAB95731"},{"key":"GeneId","value":"MPN_072"}]},{"database":"GeneID","id":"66609286","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mpn:MPN_072","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|272634.6.peg.73","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"HOGENOM","id":"CLU_109405_1_0_14","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9791329at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MPNE272634:G1GJ3-110-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000808","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0043822","properties":[{"key":"GoTerm","value":"F:ribonuclease M5 activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006364","properties":[{"key":"GoTerm","value":"P:rRNA processing"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd01027","properties":[{"key":"EntryName","value":"TOPRIM_RNase_M5_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1360.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01469","properties":[{"key":"EntryName","value":"RNase_M5"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR004466","properties":[{"key":"EntryName","value":"RNase_M5"}]},{"database":"InterPro","id":"IPR025156","properties":[{"key":"EntryName","value":"RNase_M5_C"}]},{"database":"InterPro","id":"IPR006171","properties":[{"key":"EntryName","value":"TOPRIM_dom"}]},{"database":"InterPro","id":"IPR034141","properties":[{"key":"EntryName","value":"TOPRIM_RNase_M5-like"}]},{"database":"NCBIfam","id":"TIGR00334","properties":[{"key":"EntryName","value":"5S_RNA_mat_M5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39156","properties":[{"key":"EntryName","value":"RIBONUCLEASE M5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39156:SF1","properties":[{"key":"EntryName","value":"RIBONUCLEASE M5"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13331","properties":[{"key":"EntryName","value":"DUF4093"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01751","properties":[{"key":"EntryName","value":"Toprim"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00493","properties":[{"key":"EntryName","value":"TOPRIM"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF110455","properties":[{"key":"EntryName","value":"Toprim domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50880","properties":[{"key":"EntryName","value":"TOPRIM"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDKKTRLKLDGVIVCEGKTDQARLQQLFDVSVITTNGSALNQRTINLIKAVAKKQPVILFLDPDVAGQKIRRQLEQHLDKYESCFIARKDMKPNSTKIGVAEATDAALIQALQQRQVFTKTTQPTLSWEQYLELNLNSKSKRLALCNKLHLSYFNHKQLFRKLNLLQLTFDQVCQLLK","length":178,"molWeight":20412,"crc64":"50A5129B163B86D6","md5":"44A08099613C1FB03F33930446392A15"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":1,"Binding site":4},"uniParcId":"UPI00001391B7"}}