{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P69852","secondaryAccessions":["D6VX58"],"uniProtkbId":"HSK3_YEAST","entryAudit":{"firstPublicDate":"2005-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-06-07","entryVersion":135,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"DASH complex subunit HSK3"}},"alternativeNames":[{"fullName":{"value":"Helper of ASK1 protein 3"}},{"fullName":{"value":"Outer kinetochore protein HSK3"}}]},"genes":[{"geneName":{"value":"HSK3"},"orderedLocusNames":[{"value":"YKL138C-A"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12408861"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15640796"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15664196"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16415853"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16777964"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17460120"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17643123"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18079178"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25236177"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36701236"}],"value":"Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerization to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase (PubMed:15664196, PubMed:16415853, PubMed:16777964, PubMed:17460120, PubMed:17643123). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules (PubMed:15664196, PubMed:16415853, PubMed:16777964, PubMed:17460120). Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle (PubMed:12408861). During spindle-kinetochore attachment, kinetochores first attach to the lateral surface of spindle microtubules, which supports the congression of chromosomes toward the middle of the dividing cell; they then slide along towards the spindle pole, a process independent of the DASH complex but requiring the NDC80 complex (PubMed:25236177). When the end of a disassembling microtubule reaches the laterally attached kinetochore, the DASH complex together with the NDC80 complex and STU2 convert lateral attachment to end-on capture to produce a structure that can track with microtubule shortening and sustain attachment when tension is applied across sister kinetochores upon their biorientation (PubMed:15640796, PubMed:15664196, PubMed:25236177). Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached DASH complex to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids (PubMed:16415853, PubMed:16777964). Incorrect microtubule attachments are corrected by releasing microubules from the kinetochore through phosphorylation by IPL1 of kinetochore components (PubMed:12408861). Links the microtubule cytoskeleton to chromosomes during interphase (PubMed:36701236). Also contributes to the poleward transport of kinetochores on microtubules following centromeric DNA replication in S-phase (PubMed:18079178)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94483"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15632076"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15640796"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16715078"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17460120"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17643123"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25236177"}],"value":"Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex (PubMed:15632076, PubMed:15640796, PubMed:16715078, PubMed:17460120, PubMed:17643123, PubMed:25236177). Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore (PubMed:16715078, PubMed:17460120, PubMed:17643123, PubMed:25236177). DASH complex oligomerization strengthens microtubule attachments (PubMed:25236177). On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Associates with the mitotic spindle and the kinetochore"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25236177"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25236177"}],"value":"Cytoplasm, cytoskeleton, spindle","id":"SL-0251"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25236177"}],"value":"Chromosome, centromere, kinetochore","id":"SL-0149"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25236177"}],"value":"Degron-mediated knockdown disrupts oligomerization of the DASH complex and leads to defective sister kinetochore biorientation and mitotic arrest with large 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