{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P69210","secondaryAccessions":["P77670"],"uniProtkbId":"MDTI_ECOLI","entryAudit":{"firstPublicDate":"2005-02-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-02-15","entryVersion":135,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Spermidine export protein MdtI"}}},"genes":[{"geneName":{"value":"mdtI"},"synonyms":[{"value":"ydgE"}],"orderedLocusNames":[{"value":"b1599"},{"value":"JW1591"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11566977"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"value":"Catalyzes the excretion of spermidine. Can also confer resistance to deoxycholate and SDS"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18039771"}],"value":"Forms a complex with MdtJ"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Cell inner membrane","id":"SL-0037"},"topology":{"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"value":"Induced by spermidine"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01597"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. MdtI subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Spermidine export protein MdtI","featureId":"PRO_0000108075"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"Decrease in spermidine excretion.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"Decrease in spermidine excretion.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"Decrease in spermidine excretion.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"Decrease in spermidine excretion.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["L"]}},{"type":"Mutagenesis","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"Decrease in spermidine excretion.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18039771"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["L"]}}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9097039","citationType":"journal article","authors":["Aiba H.","Baba T.","Fujita K.","Hayashi K.","Inada T.","Isono K.","Itoh T.","Kasai H.","Kashimoto K.","Kimura S.","Kitakawa M.","Kitagawa M.","Makino K.","Miki T.","Mizobuchi K.","Mori H.","Mori T.","Motomura K.","Nakade S.","Nakamura Y.","Nashimoto H.","Nishio Y.","Oshima T.","Saito N.","Sampei G.","Seki Y.","Sivasundaram S.","Tagami H.","Takeda J.","Takemoto K.","Takeuchi Y.","Wada C.","Yamamoto Y.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"9097039"},{"database":"DOI","id":"10.1093/dnares/3.6.363"}],"title":"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"363","lastPage":"377","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"11566977","citationType":"journal article","authors":["Nishino K.","Yamaguchi A."],"citationCrossReferences":[{"database":"PubMed","id":"11566977"},{"database":"DOI","id":"10.1128/jb.183.20.5803-5812.2001"}],"title":"Analysis of a complete library of putative drug transporter genes in Escherichia coli.","publicationDate":"2001","journal":"J. Bacteriol.","firstPage":"5803","lastPage":"5812","volume":"183"},"referencePositions":["FUNCTION"]},{"referenceNumber":5,"citation":{"id":"11867724","citationType":"journal article","authors":["Drew D.","Sjoestrand D.","Nilsson J.","Urbig T.","Chin C.-N.","de Gier J.-W.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"11867724"},{"database":"DOI","id":"10.1073/pnas.052018199"}],"title":"Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"2690","lastPage":"2695","volume":"99"},"referencePositions":["TOPOLOGY"],"referenceComments":[{"value":"K12 / JM109 / ATCC 53323","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":7,"citation":{"id":"18039771","citationType":"journal article","authors":["Higashi K.","Ishigure H.","Demizu R.","Uemura T.","Nishino K.","Yamaguchi A.","Kashiwagi K.","Igarashi K."],"citationCrossReferences":[{"database":"PubMed","id":"18039771"},{"database":"DOI","id":"10.1128/jb.01505-07"}],"title":"Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli.","publicationDate":"2008","journal":"J. Bacteriol.","firstPage":"872","lastPage":"878","volume":"190"},"referencePositions":["FUNCTION","SUBUNIT","INDUCTION","MUTAGENESIS OF GLU-5; GLU-19; ASP-60; TRP-68 AND TRP-81"],"referenceComments":[{"value":"K12 / C600 / CR34 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74671.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA15333.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A64916","properties":[{"key":"EntryName","value":"A64916"}]},{"database":"RefSeq","id":"NP_416116.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_000046661.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P69210","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P69210","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259124","properties":[{"key":"Interactions","value":"495"}]},{"database":"ComplexPortal","id":"CPX-6020","properties":[{"key":"EntryName","value":"MdtIJ spermidine export complex"}]},{"database":"FunCoup","id":"P69210","properties":[{"key":"Number of interactors","value":"12"}]},{"database":"IntAct","id":"P69210","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b1599","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"2.A.7.1.9","properties":[{"key":"FamilyName","value":"the drug/metabolite transporter (dmt) superfamily"}]},{"database":"PaxDb","id":"511145-b1599","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"947333","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74671","properties":[{"key":"ProteinId","value":"AAC74671"},{"key":"GeneId","value":"b1599"}]},{"database":"GeneID","id":"93775747","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"947333","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW1591","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1599","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_09210","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.663","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3685","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG2076","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_133067_0_4_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P69210","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"VAAGWIM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"71834at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P69210","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:B1599-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:B1599-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P69210","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P69210","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"ISM:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PubMed","id":"11574548"},{"evidenceCode":"ECO:0000255","source":"PubMed","id":"11867724"},{"evidenceCode":"ECO:0000255","source":"PubMed","id":"15919996"},{"evidenceCode":"ECO:0000255","source":"PubMed","id":"18039771"}]},{"database":"GO","id":"GO:1902495","properties":[{"key":"GoTerm","value":"C:transmembrane transporter complex"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"18039771"}]},{"database":"GO","id":"GO:0015199","properties":[{"key":"GoTerm","value":"F:amino-acid betaine transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0015297","properties":[{"key":"GoTerm","value":"F:antiporter activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0015220","properties":[{"key":"GoTerm","value":"F:choline transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0015606","properties":[{"key":"GoTerm","value":"F:spermidine transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18039771"}]},{"database":"GO","id":"GO:0015871","properties":[{"key":"GoTerm","value":"P:choline transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0031460","properties":[{"key":"GoTerm","value":"P:glycine betaine transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:1903711","properties":[{"key":"GoTerm","value":"P:spermidine transmembrane transport"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18039771"}]},{"database":"GO","id":"GO:1990961","properties":[{"key":"GoTerm","value":"P:xenobiotic detoxification by transmembrane export across the plasma membrane"},{"key":"GoEvidenceType","value":"IMP:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"11566977"}]},{"database":"FunFam","id":"1.10.3730.20:FF:000001","properties":[{"key":"EntryName","value":"Quaternary ammonium compound resistance transporter SugE"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.3730.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01597","properties":[{"key":"EntryName","value":"MdtI"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR037185","properties":[{"key":"EntryName","value":"EmrE-like"}]},{"database":"InterPro","id":"IPR000390","properties":[{"key":"EntryName","value":"Small_drug/metabolite_transptr"}]},{"database":"InterPro","id":"IPR045324","properties":[{"key":"EntryName","value":"Small_multidrug_res"}]},{"database":"InterPro","id":"IPR023737","properties":[{"key":"EntryName","value":"Spermidine_export_MdtI"}]},{"database":"NCBIfam","id":"NF007934","properties":[{"key":"EntryName","value":"PRK10650.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30561","properties":[{"key":"EntryName","value":"SMR FAMILY PROTON-DEPENDENT DRUG EFFLUX TRANSPORTER SUGE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30561:SF6","properties":[{"key":"EntryName","value":"SPERMIDINE EXPORT PROTEIN MDTI"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00893","properties":[{"key":"EntryName","value":"Multi_Drug_Res"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103481","properties":[{"key":"EntryName","value":"Multidrug resistance efflux transporter EmrE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAAVLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAGMIMVKLA","length":109,"molWeight":11720,"crc64":"BBDF61985CB7AE97","md5":"BA3DC74F48FA2EDFCFA472CE207310D8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":5,"Transmembrane":4,"Mutagenesis":5},"uniParcId":"UPI000013A98B"}}