{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P68460","secondaryAccessions":["P21025","Q76ZU1"],"uniProtkbId":"GLRX2_VACCW","entryAudit":{"firstPublicDate":"2004-11-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-11-23","entryVersion":87,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vaccinia virus (strain Western Reserve)","commonName":"VACV","synonyms":["Vaccinia virus (strain WR)"],"taxonId":10254,"lineage":["Viruses","Varidnaviria","Bamfordvirae","Nucleocytoviricota","Pokkesviricetes","Chitovirales","Poxviridae","Chordopoxvirinae","Orthopoxvirus","Vaccinia virus"]},"organismHosts":[{"scientificName":"Bos taurus","commonName":"Bovine","taxonId":9913}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Glutaredoxin-2"}}},"genes":[{"geneName":{"value":"OPG088"},"orderedLocusNames":[{"value":"VACWR081"}],"orfNames":[{"value":"G4L"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10982364"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11752136"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11983854"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8955061"}],"value":"Glutaredoxin necessary for virion morphogenesis and virus replication. Functions as a thiol-disulfide transfer protein between membrane-associated OPG128/A2.5 and substrates OPG095/L1 or OPG053/F9. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of OPG072/E10, OPG128/A2.5 and OPG088/G4. Exhibit thioltransferase and dehydroascorbate reductase activities in vitro"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16840349"},{"evidenceCode":"ECO:0000305"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"value":"Host cytoplasm","id":"SL-0381"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25903347"}],"value":"Expressed in the intermediate phase of the viral replicative cycle"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glutaredoxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"Glutaredoxin-2","featureId":"PRO_0000141629"},{"type":"Disulfide bond","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"Redox-active","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11752136"}]},{"type":"Mutagenesis","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":13,"modifier":"EXACT"}},"description":"Complete loss of substrate oxidation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11752136"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["S"]}},{"type":"Mutagenesis","location":{"start":{"value":16,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"Partial loss of substrate oxidation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11752136"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["S"]}},{"type":"Beta strand","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Turn","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Beta strand","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Beta strand","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Turn","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Beta strand","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]},{"type":"Helix","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2G2Q"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-1035","category":"Cellular component","name":"Host cytoplasm"},{"id":"KW-0676","category":"Domain","name":"Redox-active center"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"2024483","citationType":"journal article","authors":["Meis R.J.","Condit R.C."],"citationCrossReferences":[{"database":"PubMed","id":"2024483"},{"database":"DOI","id":"10.1016/0042-6822(91)90585-y"}],"title":"Genetic and molecular biological characterization of a vaccinia virus gene which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT).","publicationDate":"1991","journal":"Virology","firstPage":"442","lastPage":"454","volume":"182"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"CI-62F9VR5MBT7LF","citationType":"submission","authors":["Esposito J.J.","Frace A.M.","Sammons S.A.","Olsen-Rasmussen M.","Osborne J.","Wohlhueter R."],"title":"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb.","publicationDate":"FEB-2003","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"8955061","citationType":"journal article","authors":["Gvakharia B.O.","Koonin E.K.","Mathews C.K."],"citationCrossReferences":[{"database":"PubMed","id":"8955061"},{"database":"DOI","id":"10.1006/viro.1996.0669"}],"title":"Vaccinia virus G4L gene encodes a second glutaredoxin.","publicationDate":"1996","journal":"Virology","firstPage":"408","lastPage":"411","volume":"226"},"referencePositions":["FUNCTION"]},{"referenceNumber":4,"citation":{"id":"10982364","citationType":"journal article","authors":["White C.L.","Weisberg A.S.","Moss B."],"citationCrossReferences":[{"database":"PubMed","id":"10982364"},{"database":"DOI","id":"10.1128/jvi.74.19.9175-9183.2000"}],"title":"A glutaredoxin, encoded by the G4L gene of vaccinia virus, is essential for virion morphogenesis.","publicationDate":"2000","journal":"J. Virol.","firstPage":"9175","lastPage":"9183","volume":"74"},"referencePositions":["FUNCTION"]},{"referenceNumber":5,"citation":{"id":"11752136","citationType":"journal article","authors":["White C.L.","Senkevich T.G.","Moss B."],"citationCrossReferences":[{"database":"PubMed","id":"11752136"},{"database":"DOI","id":"10.1128/jvi.76.2.467-472.2002"}],"title":"Vaccinia virus G4L glutaredoxin is an essential intermediate of a cytoplasmic disulfide bond pathway required for virion assembly.","publicationDate":"2002","journal":"J. Virol.","firstPage":"467","lastPage":"472","volume":"76"},"referencePositions":["FUNCTION","DISULFIDE BOND","MUTAGENESIS OF CYS-13 AND CYS-16"]},{"referenceNumber":6,"citation":{"id":"11983854","citationType":"journal article","authors":["Senkevich T.G.","White C.L.","Koonin E.V.","Moss B."],"citationCrossReferences":[{"database":"PubMed","id":"11983854"},{"database":"DOI","id":"10.1073/pnas.062163799"}],"title":"Complete pathway for protein disulfide bond formation encoded by poxviruses.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"6667","lastPage":"6672","volume":"99"},"referencePositions":["FUNCTION"]},{"referenceNumber":7,"citation":{"id":"25903347","citationType":"journal article","authors":["Yang Z.","Cao S.","Martens C.A.","Porcella S.F.","Xie Z.","Ma M.","Shen B.","Moss B."],"citationCrossReferences":[{"database":"PubMed","id":"25903347"},{"database":"DOI","id":"10.1128/jvi.00528-15"}],"title":"Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling.","publicationDate":"2015","journal":"J. Virol.","firstPage":"6874","lastPage":"6886","volume":"89"},"referencePositions":["INDUCTION"]},{"referenceNumber":8,"citation":{"id":"16840349","citationType":"journal article","authors":["Su H.P.","Lin D.Y.","Garboczi D.N."],"citationCrossReferences":[{"database":"PubMed","id":"16840349"},{"database":"DOI","id":"10.1128/jvi.00521-06"}],"title":"The structure of G4, the poxvirus disulfide oxidoreductase essential for virus maturation and infectivity.","publicationDate":"2006","journal":"J. Virol.","firstPage":"7706","lastPage":"7713","volume":"80"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)","SUBUNIT"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"J03399","properties":[{"key":"ProteinId","value":"AAB59814.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY243312","properties":[{"key":"ProteinId","value":"AAO89360.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"YP_232963.1","properties":[{"key":"NucleotideSequenceId","value":"NC_006998.1"}]},{"database":"PDB","id":"2G2Q","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"A/B/C=1-124"}]},{"database":"PDBsum","id":"2G2Q","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P68460","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P68460","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"3707537","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"3707537","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vg:3707537","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P68460","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P68460","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000344","properties":[{"key":"Component","value":"Segment"}]},{"database":"GO","id":"GO:0030430","properties":[{"key":"GoTerm","value":"C:host cell cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"3.40.30.10","properties":[{"key":"EntryName","value":"Glutaredoxin"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008554","properties":[{"key":"EntryName","value":"Glutaredoxin-like"}]},{"database":"InterPro","id":"IPR036249","properties":[{"key":"EntryName","value":"Thioredoxin-like_sf"}]},{"database":"Pfam","id":"PF05768","properties":[{"key":"EntryName","value":"Glrx-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52833","properties":[{"key":"EntryName","value":"Thioredoxin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKNVLIIFGKPYCSICENVSDAVEELKSEYDILHVDILSFFLKDGDSSMLGDVKRGTLIGNFAAHLSNYIVSIFKYNPQTKQMAFVDINKSLDFTKTDKSLVNLEILKSEIEKATYGVWPPVTE","length":124,"molWeight":13987,"crc64":"DFBE2D7B3A1A9CA6","md5":"B2A88631E81EC3CF7F35625D1D685E51"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Disulfide bond":1,"Mutagenesis":2,"Beta strand":4,"Helix":7,"Turn":2},"uniParcId":"UPI000013849D"}}