{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P64524","secondaryAccessions":["P76365","Q2MAZ1"],"uniProtkbId":"CBTA_ECOLI","entryAudit":{"firstPublicDate":"2004-10-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-10-11","entryVersion":121,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21166897"}],"value":"Cytoskeleton-binding toxin CbtA"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21166897"}],"value":"Toxin CbtA"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14594833"}],"value":"Toxin YeeV"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21166897"}],"value":"cbtA"},"synonyms":[{"value":"yeeV"}],"orderedLocusNames":[{"value":"b2005"},{"value":"JW1987"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14594833"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22515815"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"value":"Toxic component of a type IV toxin-antitoxin (TA) system (PubMed:14594833, PubMed:21166897, PubMed:22515815, PubMed:28257056, PubMed:28931012). Acts as a dual toxin inhibitor that blocks cell division and cell elongation in genetically separable interactions with FtsZ and MreB (PubMed:28931012). Interacts with cytoskeletal proteins FtsZ and MreB; inhibits FtsZ GTP-dependent polymerization and GTPase activity as well as MreB ATP-dependent polymerization (PubMed:21166897, PubMed:28931012). Binds to both the N- and C-terminus of FtsZ, likely blocking its polymerization and localization, leading to blockage of cell division (PubMed:21166897, PubMed:28931012). Overexpression results in inhibition of growth in liquid cultures and decrease in colony formation; these effects are overcome by concomitant expression of antitoxin CbeA (YeeU) (PubMed:14594833). In other experiments cells swell, by 6 hours are lemon-shaped and by 24 hours those that have not lysed are spherical with diminished polar regions (PubMed:21166897, PubMed:28931012). Toxic effects are neutralized by cognate antitoxin CbeA, although there is no direct interaction between the 2 proteins (PubMed:14594833, PubMed:22515815). Toxic effects are also neutralized by overexpression of noncogate antitoxins YafW and YpjF (PubMed:28257056)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22515815"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"value":"Interacts with FtsZ (PubMed:21166897, PubMed:22515815, PubMed:28931012). Interacts with MreB (PubMed:21166897, PubMed:28931012)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"}],"value":"Expressed in mid-log phase at lower levels than toxin relE"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"}],"value":"The N-terminal 15 residues are required for interaction with both FtsZ and MreB, while the C-terminal 63 residues are required for interaction with MreB"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28257056"}],"value":"No visible phenotype (PubMed:21166897). Deletion of 3 type IV toxin genes (cbtA, ykfI, ypfJ) leads to a slight reduction in resistance to oxidative stress, has no effect on cell growth (PubMed:28257056)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Encoded in prophage CP4-44"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CbtA/YkfI/YpjF toxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"Cytoskeleton-binding toxin CbtA","featureId":"PRO_0000169116"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"Sufficient for toxicity, toxin less stabile; cells filament, protein does not interact with MreB but probably still interacts with FtsZ","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"}]},{"type":"Region","location":{"start":{"value":74,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"Required for interaction with MreB","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"}]},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"No longer toxic, cells become lemon-shaped more quickly than wild-type on overexpression.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21166897"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Wild-type toxicity, wild-type interaction with FtsZ, no longer interacts with MreB, no longer blocks cell elongation, cells become filamentous. No longer toxic, no altered morphology; when associated with S-65.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["C"]}},{"type":"Mutagenesis","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Slightly reduced toxicity, wild-type interaction with MreB, no longer interacts with FtsZ, no longer blocks cell division, cells become spheroid. No longer toxic, no altered morphology; when associated with C-15.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28931012"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"}],"references":[{"referenceNumber":1,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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