{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P64481","secondaryAccessions":["P76209","Q2MB45"],"uniProtkbId":"YDJM_ECOLI","entryAudit":{"firstPublicDate":"2004-10-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-11-24","entryVersion":125,"sequenceVersion":2},"annotationScore":2.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Inner membrane protein YdjM"}}},"genes":[{"geneName":{"value":"ydjM"},"orderedLocusNames":[{"value":"b1728"},{"value":"JW5281"}]}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15919996"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15919996"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10760155"}],"value":"Repressed by LexA, induced by DNA damage; it has 2 LexA binding sites both of which bind LexA with high affinity in a cooperative manner"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"To B.subtilis YvsG"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"Inner membrane protein YdjM","featureId":"PRO_0000013857"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":77,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15919996"}]}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"10760155","citationType":"journal article","authors":["Fernandez De Henestrosa A.R.","Ogi T.","Aoyagi S.","Chafin D.","Hayes J.J.","Ohmori H.","Woodgate R."],"citationCrossReferences":[{"database":"PubMed","id":"10760155"},{"database":"DOI","id":"10.1046/j.1365-2958.2000.01826.x"}],"title":"Identification of additional genes belonging to the LexA regulon in Escherichia coli.","publicationDate":"2000","journal":"Mol. Microbiol.","firstPage":"1560","lastPage":"1572","volume":"35"},"referencePositions":["REGULATION BY LEXA","INDUCTION"],"referenceComments":[{"value":"K12 / RW118","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC74798.4"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76511.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"H64931","properties":[{"key":"EntryName","value":"H64931"}]},{"database":"RefSeq","id":"NP_416242.6","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001297653.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P64481","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4261487","properties":[{"key":"Interactions","value":"66"}]},{"database":"FunCoup","id":"P64481","properties":[{"key":"Number of interactors","value":"71"}]},{"database":"STRING","id":"511145.b1728","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b1728","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC74798","properties":[{"key":"ProteinId","value":"AAC74798"},{"key":"GeneId","value":"b1728"}]},{"database":"GeneID","id":"93775941","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"945644","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW5281","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b1728","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_09880","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.528","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3745","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1988","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_097802_2_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P64481","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"TGDWWHI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"5459053at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P64481","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:G6933-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P64481","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P64481","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"}]},{"database":"GO","id":"GO:0006974","properties":[{"key":"GoTerm","value":"P:DNA damage response"},{"key":"GoEvidenceType","value":"IEP:EcoliWiki"}],"evidences":[{"evidenceCode":"ECO:0000270","source":"PubMed","id":"10760155"}]},{"database":"InterPro","id":"IPR016956","properties":[{"key":"EntryName","value":"YdjM"}]},{"database":"InterPro","id":"IPR007404","properties":[{"key":"EntryName","value":"YdjM-like_N"}]},{"database":"NCBIfam","id":"NF008651","properties":[{"key":"EntryName","value":"PRK11648.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35531","properties":[{"key":"EntryName","value":"INNER MEMBRANE PROTEIN YBCI-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35531:SF1","properties":[{"key":"EntryName","value":"INNER MEMBRANE PROTEIN YBCI-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04307","properties":[{"key":"EntryName","value":"YdjM"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF030780","properties":[{"key":"EntryName","value":"Md_memb_hyd_prd"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLLPDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADALQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSVWMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY","length":196,"molWeight":22350,"crc64":"9CB6F59FAD010235","md5":"7F0B657C1ABE847F75166FA4410F4FBC"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"INDUCTION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":5,"Transmembrane":4},"uniParcId":"UPI000018F881"}}