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Overexpression inhibits cell growth and increases the formation of persister cells. Causes 99.9% of cells to undergo bacterial lysis within 2 hours after induction; nucleoids condense, the cytoplasm seems empty and the periplasmic space enlarges. The intracellular ATP level decreases about 27-fold suggesting the membrane potential may be disrupted"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25643179"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25643179"}],"value":"By conditions that induce the stringent response such as increased expression of RelA, heat shock at 43 degrees Celsius, probably under control of sigma factor E and/or H (rpoE, rpoH), or growth in the presence of trimethoprim (TMP), mupirocin or serine hydroxamate"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17122336"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25643179"}],"value":"Strongly represses cell swarming but no effect on cell swimming (PubMed:17122336). Increased metabolic activity in the presence of TMP, an inhibitor of dihydrofolate reductase (folA) and sulfamethoxazole, an inhibitor of dihydropteroate synthetase (folC). Cells grow faster than wild-type in the presence of TMP"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25643179"}],"value":"Although this protein has high homology to GhoT, the toxic component of a type IV toxin-antitoxin system, no antitoxin has been found. GhoS (the antitoxin for GhoT), YdcY (the neighboring gene), 5'- and 3'-UTRs as well as approximately 620 bp lengths of the BW25113 genome DNA were tested"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the GhoT/OrtT toxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"Orphan toxin OrtT","featureId":"PRO_0000168933"},{"type":"Transmembrane","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Not toxic as no protein is produced.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25643179"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["T"]}},{"type":"Mutagenesis","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":38,"modifier":"EXACT"}},"description":"Reduced toxicity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25643179"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["R"]}}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0346","category":"Biological process","name":"Stress response"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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