{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P62623","secondaryAccessions":["P22565"],"uniProtkbId":"ISPH_ECOLI","entryAudit":{"firstPublicDate":"2004-07-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-19","entryVersion":160,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12706830"}],"value":"4-hydroxy-3-methylbut-2-enyl diphosphate reductase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12706830"}],"value":"HMBPP reductase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12198182"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12571359"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12706830"}],"value":"1.17.7.4"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12198182"}],"value":"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11818558"}],"value":"ispH"},"synonyms":[{"value":"lytB"},{"value":"yaaE"}],"orderedLocusNames":[{"value":"b0029"},{"value":"JW0027"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11418107"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11818558"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12706830"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19569147"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22137895"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22948824"}],"value":"Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). 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However, it initially contains a [4Fe-4S] cluster which easily degrades into a [3Fe-4S] form in the presence of oxygen (PubMed:12706830, PubMed:20080550, PubMed:22137895, PubMed:22687151)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15469281"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22012762"}],"value":"Addition of Ca(2+), Mg(2+), Mn(2+), Ni(2+), Co(2+) or Fe(2+) decreases the catalytic activity (PubMed:15469281). Addition of Zn(2+) results in complete loss of activity (PubMed:15469281). Is potently inhibited by substrate analogs in which the hydroxy group in HMBPP is replaced by an amino or thiol group (PubMed:22012762)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":30.0,"unit":"uM","substrate":"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15469281"}]}]},"phDependence":{"texts":[{"value":"Optimum pH is 7.0."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11818558"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"11418107"}],"value":"Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11818558"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"11418107"}],"value":"Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12706830"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11418107"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11717301"}],"value":"Cells lacking this gene are viable only if the medium is supplemented with mevalonate or the cells are complemented with an episomal copy of ispH (PubMed:11418107). 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