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The so-called ufmylation, requires the UFM1-activating E1 enzyme UBA5, the UFM1-conjugating E2 enzyme UFC1, and the UFM1-ligase E3 enzyme UFL1 (PubMed:15071506, PubMed:20018847, PubMed:27653677, PubMed:29868776). Ufmylation is involved in various processes, such as ribosome recycling, response to DNA damage, transcription or reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed:25219498, PubMed:32160526, PubMed:38383785)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26872069"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26929408"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27653677"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28360427"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29295865"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29868776"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30412706"}],"value":"Interacts with UBA5 (PubMed:26872069, PubMed:26929408, PubMed:27653677, PubMed:28360427, PubMed:29295865, PubMed:30412706). 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In vitro expression studies in different cell lines showed that the mutation significantly reduces transcriptional activity in certain neuronal cell lines (SY5Y and U373), but not in other cell lines, including HeLa and HOF-F2"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UFM1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"Ubiquitin-fold modifier 1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15071506"}],"featureId":"PRO_0000042122"},{"type":"Propeptide","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"Removed in mature 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