{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P61714","secondaryAccessions":["P25540","P77114","Q2MC11"],"uniProtkbId":"RISB_ECOLI","entryAudit":{"firstPublicDate":"2004-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-06-07","entryVersion":163,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"6,7-dimethyl-8-ribityllumazine synthase"},"shortNames":[{"value":"DMRL synthase"},{"value":"LS"},{"value":"Lumazine synthase"}],"ecNumbers":[{"value":"2.5.1.78"}]}},"genes":[{"geneName":{"value":"ribE"},"synonyms":[{"value":"ribH"},{"value":"ybaF"}],"orderedLocusNames":[{"value":"b0415"},{"value":"JW0405"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}],"value":"Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:26152"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15934"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58201"},{"database":"ChEBI","id":"CHEBI:58830"}],"ecNumber":"2.5.1.78","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":11800.0,"unit":"nmol/h/mg","enzyme":"enzyme","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}]}],"michaelisConstants":[{"constant":4.2,"unit":"uM","substrate":"5-amino-6-(D-ribitylamino)uracil","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}]},{"constant":62.0,"unit":"uM","substrate":"3,4-dihydroxy-2-butanone 4-phosphate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}]}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}],"value":"Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8969176"}],"value":"Forms a hollow icosahedral capsid composed of 60 subunits, probably arranged as a dodecamer of pentamers. Unlike in B.subtilis, does not interact with riboflavin synthase, and the core of the icosahedral capsid is empty"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DMRL synthase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":156,"modifier":"EXACT"}},"description":"6,7-dimethyl-8-ribityllumazine synthase","featureId":"PRO_0000134754"},{"type":"Active site","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Binding site","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15934"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"5-amino-6-(D-ribitylamino)uracil","id":"ChEBI:CHEBI:15934"}},{"type":"Binding site","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":59,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15934"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"5-amino-6-(D-ribitylamino)uracil","id":"ChEBI:CHEBI:15934"}},{"type":"Binding site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15934"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"5-amino-6-(D-ribitylamino)uracil","id":"ChEBI:CHEBI:15934"}},{"type":"Binding site","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58830"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"(2S)-2-hydroxy-3-oxobutyl phosphate","id":"ChEBI:CHEBI:58830"}},{"type":"Binding site","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15934"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"5-amino-6-(D-ribitylamino)uracil","id":"ChEBI:CHEBI:15934"}},{"type":"Binding site","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58830"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"(2S)-2-hydroxy-3-oxobutyl phosphate","id":"ChEBI:CHEBI:58830"}}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0686","category":"Biological process","name":"Riboflavin biosynthesis"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"1406588","citationType":"journal article","authors":["Taura T.","Ueguchi C.","Shiba K.","Ito K."],"citationCrossReferences":[{"database":"PubMed","id":"1406588"},{"database":"DOI","id":"10.1007/bf00538702"}],"title":"Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation.","publicationDate":"1992","journal":"Mol. Gen. Genet.","firstPage":"429","lastPage":"432","volume":"234"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"K12","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"CI-B1UJ6TNQ0SAS1","citationType":"submission","authors":["Chung E.","Allen E.","Araujo R.","Aparicio A.M.","Davis K.","Duncan M.","Federspiel N.","Hyman R.","Kalman S.","Komp C.","Kurdi O.","Lew H.","Lin D.","Namath A.","Oefner P.","Roberts D.","Schramm S.","Davis R.W."],"title":"Sequence of minutes 4-25 of Escherichia coli.","publicationDate":"JAN-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"8969176","citationType":"journal article","authors":["Moertl S.","Fischer M.","Richter G.","Tack J.","Weinkauf S.","Bacher A."],"citationCrossReferences":[{"database":"PubMed","id":"8969176"},{"database":"DOI","id":"10.1074/jbc.271.52.33201"}],"title":"Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli.","publicationDate":"1996","journal":"J. Biol. Chem.","firstPage":"33201","lastPage":"33207","volume":"271"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","KINETIC PARAMETERS","SUBUNIT","PATHWAY"],"referenceComments":[{"value":"K12 / RR28","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X64395","properties":[{"key":"ProteinId","value":"CAA45736.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U82664","properties":[{"key":"ProteinId","value":"AAB40171.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73518.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE76195.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S26202","properties":[{"key":"EntryName","value":"S26202"}]},{"database":"RefSeq","id":"NP_414949.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001021161.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"AlphaFoldDB","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259505","properties":[{"key":"Interactions","value":"94"}]},{"database":"DIP","id":"DIP-10711N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P61714","properties":[{"key":"Number of interactors","value":"791"}]},{"database":"IntAct","id":"P61714","properties":[{"key":"Interactions","value":"16"}]},{"database":"STRING","id":"511145.b0415","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b0415","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73518","properties":[{"key":"ProteinId","value":"AAC73518"},{"key":"GeneId","value":"b0415"}]},{"database":"GeneID","id":"946453","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"98391920","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW0405","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0415","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_02025","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.1862","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB1298","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG0054","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_089358_1_1_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"CQGVTQG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9809709at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:LUMAZINESYN-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:LUMAZINESYN-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"SABIO-RK","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00275","properties":[{"key":"RectionId","value":"UER00404"}]},{"database":"PRO","id":"PR:P61714","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P61714","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15911532"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18304323"}]},{"database":"GO","id":"GO:0009349","properties":[{"key":"GoTerm","value":"C:riboflavin synthase complex"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0000906","properties":[{"key":"GoTerm","value":"F:6,7-dimethyl-8-ribityllumazine synthase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8969176"}]},{"database":"GO","id":"GO:0009231","properties":[{"key":"GoTerm","value":"P:riboflavin biosynthetic process"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8969176"}]},{"database":"CDD","id":"cd09209","properties":[{"key":"EntryName","value":"Lumazine_synthase-I"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.960:FF:000001","properties":[{"key":"EntryName","value":"6,7-dimethyl-8-ribityllumazine synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.960","properties":[{"key":"EntryName","value":"Lumazine/riboflavin synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00178","properties":[{"key":"EntryName","value":"Lumazine_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR034964","properties":[{"key":"EntryName","value":"LS"}]},{"database":"InterPro","id":"IPR002180","properties":[{"key":"EntryName","value":"LS/RS"}]},{"database":"InterPro","id":"IPR036467","properties":[{"key":"EntryName","value":"LS/RS_sf"}]},{"database":"NCBIfam","id":"TIGR00114","properties":[{"key":"EntryName","value":"lumazine-synth"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF000812","properties":[{"key":"EntryName","value":"PRK00061.1-4"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21058:SF0","properties":[{"key":"EntryName","value":"6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21058","properties":[{"key":"EntryName","value":"6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00885","properties":[{"key":"EntryName","value":"DMRL_synthase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52121","properties":[{"key":"EntryName","value":"Lumazine synthase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA","length":156,"molWeight":16157,"crc64":"1F8504B2892195C7","md5":"726E62E250BBD136FACA4FD18D794103"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Binding site":6},"uniParcId":"UPI00000000D1"}}