{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P61165","secondaryAccessions":["A8K6L8","Q9D953","Q9Y2Q7"],"uniProtkbId":"TM258_HUMAN","entryAudit":{"firstPublicDate":"2004-05-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-05-10","entryVersion":146,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258"},"shortNames":[{"value":"Oligosaccharyl transferase subunit TMEM258"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Transmembrane protein 258"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:1164"}],"value":"TMEM258"},"synonyms":[{"value":"C11orf10"}],"orfNames":[{"value":"HSPC005"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P61166"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26472760"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27974209"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31831667"}],"value":"Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (PubMed:26472760, PubMed:27974209). Involved in ER homeostasis in the colonic epithelium (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31831667"}],"value":"Protein modification; protein glycosylation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E2RKN8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26472760"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27974209"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28716842"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31831667"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36697828"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38670073"}],"value":"Component of the oligosaccharyltransferase (OST) complex (PubMed:31831667, PubMed:36697828, PubMed:38670073). OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits (PubMed:26472760, PubMed:27974209, PubMed:31831667, PubMed:36697828, PubMed:38670073). STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity). Interacts with SYNE1 (via KASH domain), SYNE2 (via KASH domain) and SUN1 (PubMed:28716842)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P61165","intActId":"EBI-12019210"},"interactantTwo":{"uniProtKBAccession":"Q15041","geneName":"ARL6IP1","intActId":"EBI-714543"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P61165","intActId":"EBI-12019210"},"interactantTwo":{"uniProtKBAccession":"Q9Y5U9","geneName":"IER3IP1","intActId":"EBI-725665"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P61165","intActId":"EBI-12019210"},"interactantTwo":{"uniProtKBAccession":"Q6ZSS7","geneName":"MFSD6","intActId":"EBI-2858252"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P61165","intActId":"EBI-12019210"},"interactantTwo":{"uniProtKBAccession":"Q9UMX0","geneName":"UBQLN1","intActId":"EBI-741480"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P61165","intActId":"EBI-12019210"},"interactantTwo":{"uniProtKBAccession":"P0DTC4","geneName":"E","intActId":"EBI-25475850"},"numberOfExperiments":3,"organismDiffer":true}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28716842"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26472760"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27974209"}],"value":"Endoplasmic reticulum","id":"SL-0095"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12427278"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28716842"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27974209"}],"value":"Ubiquitously expressed"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the OST5 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit TMEM258","featureId":"PRO_0000221142"},{"type":"Transmembrane","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Modified residue","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"N-acetylmethionine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19413330"},{"evidenceCode":"ECO:0007744","source":"PubMed","id":"22223895"}]},{"type":"Beta strand","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":8,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6S7T"}]},{"type":"Helix","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6S7T"}]},{"type":"Helix","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6S7O"}]},{"type":"Helix","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6S7O"}]},{"type":"Helix","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6S7O"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6S7O"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-DKB2OI2FC6KMJ","citationType":"submission","authors":["Marquardt A.","Stoehr H.","Rivera A.","Weber B.H.F."],"title":"Identification and genomic characterization of seven novel genes from a 288 kb interval in 11q12-q13.1.","publicationDate":"AUG-1998","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":2,"citation":{"id":"11042152","citationType":"journal article","authors":["Zhang Q.-H.","Ye M.","Wu X.-Y.","Ren S.-X.","Zhao M.","Zhao C.-J.","Fu G.","Shen Y.","Fan H.-Y.","Lu G.","Zhong M.","Xu X.-R.","Han Z.-G.","Zhang J.-W.","Tao J.","Huang Q.-H.","Zhou J.","Hu G.-X.","Gu J.","Chen S.-J.","Chen Z."],"citationCrossReferences":[{"database":"PubMed","id":"11042152"},{"database":"DOI","id":"10.1101/gr.140200"}],"title":"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.","publicationDate":"2000","journal":"Genome Res.","firstPage":"1546","lastPage":"1560","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Umbilical cord blood","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Placenta","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"16554811","citationType":"journal article","authors":["Taylor T.D.","Noguchi H.","Totoki Y.","Toyoda A.","Kuroki Y.","Dewar K.","Lloyd C.","Itoh T.","Takeda T.","Kim D.-W.","She X.","Barlow K.F.","Bloom T.","Bruford E.","Chang J.L.","Cuomo C.A.","Eichler E.","FitzGerald M.G.","Jaffe D.B.","LaButti K.","Nicol R.","Park H.-S.","Seaman C.","Sougnez C.","Yang X.","Zimmer A.R.","Zody M.C.","Birren B.W.","Nusbaum C.","Fujiyama A.","Hattori M.","Rogers J.","Lander E.S.","Sakaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16554811"},{"database":"DOI","id":"10.1038/nature04632"}],"title":"Human chromosome 11 DNA sequence and analysis including novel gene identification.","publicationDate":"2006","journal":"Nature","firstPage":"497","lastPage":"500","volume":"440"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":5,"citation":{"id":"CI-5GBDQ6B103N1E","citationType":"submission","authors":["Mural R.J.","Istrail S.","Sutton G.G.","Florea L.","Halpern A.L.","Mobarry C.M.","Lippert R.","Walenz B.","Shatkay H.","Dew I.","Miller J.R.","Flanigan M.J.","Edwards N.J.","Bolanos R.","Fasulo D.","Halldorsson B.V.","Hannenhalli S.","Turner R.","Yooseph S.","Lu F.","Nusskern D.R.","Shue B.C.","Zheng X.H.","Zhong F.","Delcher A.L.","Huson D.H.","Kravitz S.A.","Mouchard L.","Reinert K.","Remington K.A.","Clark A.G.","Waterman M.S.","Eichler E.E.","Adams M.D.","Hunkapiller M.W.","Myers E.W.","Venter J.C."],"publicationDate":"JUL-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":6,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Uterus","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"12665801","citationType":"journal article","authors":["Gevaert K.","Goethals M.","Martens L.","Van Damme J.","Staes A.","Thomas G.R.","Vandekerckhove J."],"citationCrossReferences":[{"database":"PubMed","id":"12665801"},{"database":"DOI","id":"10.1038/nbt810"}],"title":"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.","publicationDate":"2003","journal":"Nat. Biotechnol.","firstPage":"566","lastPage":"569","volume":"21"},"referencePositions":["PROTEIN SEQUENCE OF 1-8"],"referenceComments":[{"value":"Platelet","type":"TISSUE"}]},{"referenceNumber":8,"citation":{"id":"12427278","citationType":"journal article","authors":["Adachi N.","Karanjawala Z.E.","Matsuzaki Y.","Koyama H.","Lieber M.R."],"citationCrossReferences":[{"database":"PubMed","id":"12427278"},{"database":"DOI","id":"10.1089/15362310260256927"}],"title":"Two overlapping divergent transcription units in the human genome: the FEN1/C11orf10 locus.","publicationDate":"2002","journal":"OMICS","firstPage":"273","lastPage":"279","volume":"6"},"referencePositions":["SUBCELLULAR LOCATION"]},{"referenceNumber":9,"citation":{"id":"19413330","citationType":"journal article","authors":["Gauci S.","Helbig A.O.","Slijper M.","Krijgsveld J.","Heck A.J.","Mohammed S."],"citationCrossReferences":[{"database":"PubMed","id":"19413330"},{"database":"DOI","id":"10.1021/ac9004309"}],"title":"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.","publicationDate":"2009","journal":"Anal. Chem.","firstPage":"4493","lastPage":"4501","volume":"81"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":10,"citation":{"id":"22223895","citationType":"journal article","authors":["Bienvenut W.V.","Sumpton D.","Martinez A.","Lilla S.","Espagne C.","Meinnel T.","Giglione C."],"citationCrossReferences":[{"database":"PubMed","id":"22223895"},{"database":"DOI","id":"10.1074/mcp.m111.015131"}],"title":"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.","publicationDate":"2012","journal":"Mol. Cell. Proteomics","firstPage":"M111.015131","lastPage":"M111.015131","volume":"11"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":11,"citation":{"id":"22814378","citationType":"journal article","authors":["Van Damme P.","Lasa M.","Polevoda B.","Gazquez C.","Elosegui-Artola A.","Kim D.S.","De Juan-Pardo E.","Demeyer K.","Hole K.","Larrea E.","Timmerman E.","Prieto J.","Arnesen T.","Sherman F.","Gevaert K.","Aldabe R."],"citationCrossReferences":[{"database":"PubMed","id":"22814378"},{"database":"DOI","id":"10.1073/pnas.1210303109"}],"title":"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.","publicationDate":"2012","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"12449","lastPage":"12454","volume":"109"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":12,"citation":{"id":"26472760","citationType":"journal article","authors":["Blomen V.A.","Majek P.","Jae L.T.","Bigenzahn J.W.","Nieuwenhuis J.","Staring J.","Sacco R.","van Diemen F.R.","Olk N.","Stukalov A.","Marceau C.","Janssen H.","Carette J.E.","Bennett K.L.","Colinge J.","Superti-Furga G.","Brummelkamp T.R."],"citationCrossReferences":[{"database":"PubMed","id":"26472760"},{"database":"DOI","id":"10.1126/science.aac7557"}],"title":"Gene essentiality and synthetic lethality in haploid human cells.","publicationDate":"2015","journal":"Science","firstPage":"1092","lastPage":"1096","volume":"350"},"referencePositions":["FUNCTION","IDENTIFICATION IN THE OLIGOSACCHARYLTRANSFERASE COMPLEX","SUBCELLULAR LOCATION"]},{"referenceNumber":13,"citation":{"id":"27974209","citationType":"journal article","authors":["Graham D.B.","Lefkovith A.","Deelen P.","de Klein N.","Varma M.","Boroughs A.","Desch A.N.","Ng A.C.","Guzman G.","Schenone M.","Petersen C.P.","Bhan A.K.","Rivas M.A.","Daly M.J.","Carr S.A.","Wijmenga C.","Xavier R.J."],"citationCrossReferences":[{"database":"PubMed","id":"27974209"},{"database":"DOI","id":"10.1016/j.celrep.2016.11.042"}],"title":"TMEM258 is a component of the oligosaccharyltransferase complex controlling ER stress and intestinal inflammation.","publicationDate":"2016","journal":"Cell Rep.","firstPage":"2955","lastPage":"2965","volume":"17"},"referencePositions":["FUNCTION","TISSUE SPECIFICITY","SUBCELLULAR LOCATION","IDENTIFICATION IN THE OLIGOSACCHARYLTRANSFERASE COMPLEX","INTERACTION WITH RPN1"]},{"referenceNumber":14,"citation":{"id":"28716842","citationType":"journal article","authors":["Ding Z.Y.","Wang Y.H.","Huang Y.C.","Lee M.C.","Tseng M.J.","Chi Y.H.","Huang M.L."],"citationCrossReferences":[{"database":"PubMed","id":"28716842"},{"database":"DOI","id":"10.1083/jcb.201606043"}],"title":"Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture.","publicationDate":"2017","journal":"J. Cell Biol.","firstPage":"2827","lastPage":"2841","volume":"216"},"referencePositions":["SUBCELLULAR LOCATION","INTERACTION WITH SYNE1; SYNE2 AND SUN1"]},{"referenceNumber":15,"citation":{"id":"31831667","citationType":"journal article","authors":["Ramirez A.S.","Kowal J.","Locher K.P."],"citationCrossReferences":[{"database":"PubMed","id":"31831667"},{"database":"DOI","id":"10.1126/science.aaz3505"}],"title":"Cryo-electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B.","publicationDate":"2019","journal":"Science","firstPage":"1372","lastPage":"1375","volume":"366"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS)","IDENTIFICATION IN THE OLIGOSACCHARYL TRANSFERASE (OST) COMPLEX","FUNCTION","PATHWAY"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"6S7O"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"6S7T"}]},{"referenceNumber":16,"citation":{"id":"36697828","citationType":"journal article","authors":["Gemmer M.","Chaillet M.L.","van Loenhout J.","Cuevas Arenas R.","Vismpas D.","Grollers-Mulderij M.","Koh F.A.","Albanese P.","Scheltema R.A.","Howes S.C.","Kotecha A.","Fedry J.","Forster F."],"citationCrossReferences":[{"database":"PubMed","id":"36697828"},{"database":"DOI","id":"10.1038/s41586-022-05638-5"}],"title":"Visualization of translation and protein biogenesis at the ER membrane.","publicationDate":"2023","journal":"Nature","firstPage":"160","lastPage":"167","volume":"614"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (7.60 ANGSTROMS) OF THE STT3A-CONTAINING OLIGOSACCHARYLTRANSFERASE (OST) AND TRANSLOCON COMPLEXES","SUBUNIT"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"8B6L"}]},{"referenceNumber":17,"citation":{"id":"38670073","citationType":"journal article","authors":["Lampson B.L.","Ramrez A.S.","Baro M.","He L.","Hegde M.","Koduri V.","Pfaff J.L.","Hanna R.E.","Kowal J.","Shirole N.H.","He Y.","Doench J.G.","Contessa J.N.","Locher K.P.","Kaelin W.G."],"citationCrossReferences":[{"database":"PubMed","id":"38670073"},{"database":"DOI","id":"10.1016/j.cell.2024.03.022"}],"title":"Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-kappaB.","publicationDate":"2024","journal":"Cell","firstPage":"2209","lastPage":"2223","volume":"187"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.61 ANGSTROMS) OF THE STT3A-CONTAINING OLIGOSACCHARYLTRANSFERASE (OST)","SUBUNIT"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"8PN9"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF086763","properties":[{"key":"ProteinId","value":"AAF28401.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF070661","properties":[{"key":"ProteinId","value":"AAD20967.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK291683","properties":[{"key":"ProteinId","value":"BAF84372.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AP002380","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CH471076","properties":[{"key":"ProteinId","value":"EAW73970.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC002750","properties":[{"key":"ProteinId","value":"AAH02750.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC015968","properties":[{"key":"ProteinId","value":"AAH15968.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS8009.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_055021.1","properties":[{"key":"NucleotideSequenceId","value":"NM_014206.4"}]},{"database":"PDB","id":"6S7O","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.50 A"},{"key":"Chains","value":"C=1-79"}]},{"database":"PDB","id":"6S7T","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.50 A"},{"key":"Chains","value":"C=1-79"}]},{"database":"PDB","id":"8B6L","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"7.60 A"},{"key":"Chains","value":"L=1-79"}]},{"database":"PDB","id":"8PN9","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.61 A"},{"key":"Chains","value":"C=1-79"}]},{"database":"PDB","id":"9N9J","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"L2=1-79"}]},{"database":"PDB","id":"9YGY","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"4.10 A"},{"key":"Chains","value":"L2=1-79"}]},{"database":"PDBsum","id":"6S7O","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6S7T","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8B6L","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8PN9","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9N9J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9YGY","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-10110","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-10112","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-15870","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-17779","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-49171","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-72945","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"107204","properties":[{"key":"Interactions","value":"47"}]},{"database":"ComplexPortal","id":"CPX-5621","properties":[{"key":"EntryName","value":"Oligosaccharyltransferase complex A"}]},{"database":"ComplexPortal","id":"CPX-5622","properties":[{"key":"EntryName","value":"Oligosaccharyltransferase complex B, MAGT1 variant"}]},{"database":"ComplexPortal","id":"CPX-8738","properties":[{"key":"EntryName","value":"Oligosaccharyltransferase complex B, TUCS3 variant"}]},{"database":"FunCoup","id":"P61165","properties":[{"key":"Number of interactors","value":"840"}]},{"database":"IntAct","id":"P61165","properties":[{"key":"Interactions","value":"26"}]},{"database":"NDEx","id":"MUSIC2-C5180-TMEM258","properties":[{"key":"Evidences","value":"Osteosarcoma (U2OS) cell map - C5180 (62 proteins)"}]},{"database":"STRING","id":"9606.ENSP00000443216","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"9.B.142.3.17","properties":[{"key":"FamilyName","value":"the integral membrane glycosyltransferase family 39 (gt39) family"}]},{"database":"GlyGen","id":"P61165","properties":[{"key":"glycosylation","value":"1 site, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"TMEM258","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"47117653","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000443216","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"57271","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"TopDownProteomics","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"51765","properties":[{"key":"antibodies","value":"9 antibodies from 7 providers"}]},{"database":"DNASU","id":"746","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000257262.12","properties":[{"key":"ProteinId","value":"ENSP00000257262.8"},{"key":"GeneId","value":"ENSG00000134825.17"}]},{"database":"Ensembl","id":"ENST00000537328.6","properties":[{"key":"ProteinId","value":"ENSP00000443216.1"},{"key":"GeneId","value":"ENSG00000134825.17"}]},{"database":"Ensembl","id":"ENST00000857009.1","properties":[{"key":"ProteinId","value":"ENSP00000527068.1"},{"key":"GeneId","value":"ENSG00000134825.17"}]},{"database":"Ensembl","id":"ENST00000947562.1","properties":[{"key":"ProteinId","value":"ENSP00000617621.1"},{"key":"GeneId","value":"ENSG00000134825.17"}]},{"database":"GeneID","id":"746","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:746","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000537328.6","properties":[{"key":"ProteinId","value":"ENSP00000443216.1"},{"key":"RefSeqNucleotideId","value":"NM_014206.4"},{"key":"RefSeqProteinId","value":"NP_055021.1"}]},{"database":"UCSC","id":"uc001nsf.4","properties":[{"key":"OrganismName","value":"human"}]},{"database":"AGR","id":"HGNC:1164","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA25478","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"746","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"746","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"TMEM258","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:1164","properties":[{"key":"GeneName","value":"TMEM258"}]},{"database":"HPA","id":"ENSG00000134825","properties":[{"key":"ExpressionPatterns","value":"Low tissue specificity"}]},{"database":"MIM","id":"617615","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000134825","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000134825","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4452","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000010089","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MERYVGP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"18408at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"P61165","properties":[{"key":"Number of GO annotations","value":"3 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.4.99.18","properties":[{"key":"OrganismId","value":"2681"}]},{"database":"PathwayCommons","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-HSA-446203","properties":[{"key":"PathwayName","value":"Asparagine N-linked glycosylation"}]},{"database":"Reactome","id":"R-HSA-9694548","properties":[{"key":"PathwayName","value":"Maturation of spike protein"}]},{"database":"Reactome","id":"R-HSA-9768727","properties":[{"key":"PathwayName","value":"Regulation of CDH1 posttranslational processing and trafficking to plasma membrane"}]},{"database":"Reactome","id":"R-HSA-9918432","properties":[{"key":"PathwayName","value":"Maturation of DENV proteins"}]},{"database":"Reactome","id":"R-HSA-9931295","properties":[{"key":"PathwayName","value":"PD-L1(CD274) glycosylation and translocation to plasma membrane"}]},{"database":"SignaLink","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"SIGNOR","id":"P61165","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00378","properties":[{"key":"RectionId","value":"-"}]},{"database":"Agora","id":"ENSG00000134825","properties":[{"key":"NominatedTarget","value":"Agora Nominated Target for Alzheimer's Disease"}]},{"database":"BioGRID-ORCS","id":"746","properties":[{"key":"hits","value":"613 hits in 1162 CRISPR screens"}]},{"database":"ChiTaRS","id":"TMEM258","properties":[{"key":"OrganismName","value":"human"}]},{"database":"GenomeRNAi","id":"746","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"P61165","properties":[{"key":"DevelopmentLevel","value":"Tdark"}]},{"database":"PRO","id":"PR:P61165","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 11"}]},{"database":"RNAct","id":"P61165","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000134825","properties":[{"key":"ExpressionPatterns","value":"Expressed in type B pancreatic cell and 205 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"P61165","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12427278"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"28716842"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26472760"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"28716842"}]},{"database":"GO","id":"GO:0008250","properties":[{"key":"GoTerm","value":"C:oligosaccharyltransferase complex"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26472760"}]},{"database":"GO","id":"GO:0160226","properties":[{"key":"GoTerm","value":"C:oligosaccharyltransferase complex A"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31831667"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36697828"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38670073"}]},{"database":"GO","id":"GO:0160227","properties":[{"key":"GoTerm","value":"C:oligosaccharyltransferase complex B"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31831667"}]},{"database":"GO","id":"GO:0062062","properties":[{"key":"GoTerm","value":"F:oligosaccharyltransferase complex binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0006487","properties":[{"key":"GoTerm","value":"P:protein N-linked glycosylation"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31831667"}]},{"database":"GO","id":"GO:0009306","properties":[{"key":"GoTerm","value":"P:protein secretion"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27974209"}]},{"database":"GO","id":"GO:0034976","properties":[{"key":"GoTerm","value":"P:response to endoplasmic reticulum stress"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27974209"}]},{"database":"InterPro","id":"IPR007915","properties":[{"key":"EntryName","value":"TMEM258/Ost5"}]},{"database":"PANTHER","id":"PTHR13636","properties":[{"key":"EntryName","value":"TRANSMEMBRANE PROTEIN 258"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05251","properties":[{"key":"EntryName","value":"Ost5"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MELEAMSRYTSPVNPAVFPHLTVVLLAIGMFFTAWFFVYEVTSTKYTRDIYKELLISLVASLFMGFGVLFLLLWVGIYV","length":79,"molWeight":9079,"crc64":"8105E40132994646","md5":"BB5BADD6E553CCE6D7244FD7639D6C86"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"PATHWAY":1,"SUBUNIT":1,"INTERACTION":5,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":2,"Modified residue":1,"Beta strand":1,"Helix":5},"uniParcId":"UPI000000109F"}}