{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P60357","secondaryAccessions":["Q92BL0"],"uniProtkbId":"REOM_LISMO","entryAudit":{"firstPublicDate":"2004-02-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-02-16","entryVersion":99,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)","taxonId":169963,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Listeriaceae","Listeria"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"32469310"}],"value":"Peptidoglycan synthesis regulatory protein ReoM"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32469310"}],"value":"Regulator of MurA(A) degradation"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"32469310"}],"value":"reoM"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11679669"}],"value":"lmo1503"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34624065"}],"value":"Involved in the PASTA kinase-mediated signaling pathway regulating peptidoglycan synthesis to maintain cell wall integrity (PubMed:32469310, PubMed:34624065). Modulates peptidoglycan (PG) synthesis pathway committed-step enzyme MurA (PubMed:32469310, PubMed:34624065). Positively modulates ClpCP-dependent proteolysis of MurA, thereby regulating PG synthesis and inhibiting intrinsic cephalosporin resistance (PubMed:32469310, PubMed:34624065). Plays a role in virulence in mice (PubMed:34624065)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39245639"}],"value":"Homodimer (PubMed:32469310). Interacts directly with MurA, probably in a substrate-dependent manner; interaction is reduced by phosphorylation of ReoM (PubMed:32469310, PubMed:39245639). Interacts directly with ReoY; interaction may bridge the interaction of ClpCP with its substrate MurA (PubMed:32469310)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39245639"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"34624065"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"39245639"}],"value":"Phosphorylated by Ser/Thr protein kinase PrkA; absence of phosphorylation of Thr-7 may reduce viability, in a ClpC-dependent manner (Probable) (PubMed:32469310). Phosphorylated in a GpsB-dependent manner, but seems independent of general disruption of cell-division (PubMed:39245639). Phosphorylation level declines at the stationary phase (PubMed:39245639). Levels of peptidoglycan (PG) synthesis enzyme MurA may provide a negative feedback loop on phosphorylation of ReoM (PubMed:39245639). Dephosphorylated by protein phosphatase PrpC (PubMed:32469310, PubMed:39245639)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34624065"}],"value":"Increases flux through the peptidoglycan (PG) synthesis pathway (PubMed:34624065). Thicker PG layers at the cell poles and more uneven PG layers along the lateral wall (PubMed:32469310). Increases resistance to cephalosporin antibiotic ceftriaxone about two- to three-fold; resistance returns to about wild-type levels in a prkA mutant background (PubMed:32469310, PubMed:34624065). Slightly increases resistance to ampicillin and antimicrobial peptide LL-37; resistance returns to about wild-type levels in a prkA mutant background (PubMed:34624065). Salt-sensitive growth (PubMed:32469310). Deletion has no effect on growth, but completely suppresses the growth defects of a gpsB mutant at both 37 and 42 degrees Celsius (PubMed:32469310). Cell length is normal; similar phenotype in a gpsB mutant background (PubMed:32469310). Normal rod-shape, but partially bent in a gpsB mutant background (PubMed:32469310). MurA protein levels increase by at least eight-fold; expression level of MurA increases similarly in clpC, reoY or murZ/murA2 mutant backgrounds (PubMed:32469310). Similar levels of intracellular lysis as wild-type, when grown in bone-marrow derived C57BL/6 strain mouse macrophages; unchanged in a prkA mutant background (PubMed:34624065). Forms plaques about 50% of wild-type size when grown on a monolayer of mouse immortalized L2 fibroblasts (PubMed:34624065). Growth almost the same as wild-type in spleen, but substantially less (about 1000-fold) in liver, in a mouse C57BL/6 intravenous infection model (PubMed:34624065)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01507"}],"value":"Belongs to the IreB/ReoM family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"Peptidoglycan synthesis regulatory protein ReoM","featureId":"PRO_0000216974"},{"type":"Modified residue","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34624065"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39245639"}]},{"type":"Mutagenesis","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"Abolishes phosphorylation by PrkA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"Expression is lethal.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":62,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"Expression is lethal.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32469310"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Helix","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TIF"}]},{"type":"Helix","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TIF"}]},{"type":"Helix","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TIF"}]},{"type":"Helix","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TIF"}]},{"type":"Helix","location":{"start":{"value":70,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6TIF"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"11679669","citationType":"journal article","authors":["Glaser P.","Frangeul L.","Buchrieser C.","Rusniok C.","Amend A.","Baquero F.","Berche P.","Bloecker H.","Brandt P.","Chakraborty T.","Charbit A.","Chetouani F.","Couve E.","de Daruvar A.","Dehoux P.","Domann E.","Dominguez-Bernal G.","Duchaud E.","Durant L.","Dussurget O.","Entian K.-D.","Fsihi H.","Garcia-del Portillo F.","Garrido P.","Gautier L.","Goebel W.","Gomez-Lopez N.","Hain T.","Hauf J.","Jackson D.","Jones L.-M.","Kaerst U.","Kreft J.","Kuhn M.","Kunst F.","Kurapkat G.","Madueno E.","Maitournam A.","Mata Vicente J.","Ng E.","Nedjari H.","Nordsiek G.","Novella S.","de Pablos B.","Perez-Diaz J.-C.","Purcell R.","Remmel B.","Rose M.","Schlueter T.","Simoes N.","Tierrez A.","Vazquez-Boland J.-A.","Voss H.","Wehland J.","Cossart P."],"citationCrossReferences":[{"database":"PubMed","id":"11679669"},{"database":"DOI","id":"10.1126/science.1063447"}],"title":"Comparative genomics of Listeria species.","publicationDate":"2001","journal":"Science","firstPage":"849","lastPage":"852","volume":"294"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC BAA-679 / EGD-e","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"34624065","citationType":"journal article","authors":["Kelliher J.L.","Grunenwald C.M.","Abrahams R.R.","Daanen M.E.","Lew C.I.","Rose W.E.","Sauer J.D."],"citationCrossReferences":[{"database":"PubMed","id":"34624065"},{"database":"DOI","id":"10.1371/journal.ppat.1009881"}],"title":"PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall stress responses, cytosolic survival, and virulence in Listeria monocytogenes.","publicationDate":"2021","journal":"PLoS Pathog.","firstPage":"e1009881","lastPage":"e1009881","volume":"17"},"referencePositions":["FUNCTION","PHOSPHORYLATION AT THR-7","DISRUPTION PHENOTYPE"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":3,"citation":{"id":"39245639","citationType":"journal article","authors":["Rothe P.","Wamp S.","Rosemeyer L.","Rismondo J.","Doellinger J.","Gruendling A.","Halbedel S."],"citationCrossReferences":[{"database":"PubMed","id":"39245639"},{"database":"DOI","id":"10.1111/mmi.15307"}],"title":"Cytosolic Factors Controlling PASTA Kinase-Dependent ReoM Phosphorylation.","publicationDate":"2024","journal":"Mol. Microbiol.","firstPage":"514","lastPage":"533","volume":"122"},"referencePositions":["INTERACTION WITH MURA","PHOSPHORYLATION AT THR-7"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":4,"citation":{"id":"32469310","citationType":"journal article","authors":["Wamp S.","Rutter Z.J.","Rismondo J.","Jennings C.E.","Moller L.","Lewis R.J.","Halbedel S."],"citationCrossReferences":[{"database":"PubMed","id":"32469310"},{"database":"DOI","id":"10.7554/elife.56048"}],"title":"PrkA controls peptidoglycan biosynthesis through the essential phosphorylation of ReoM.","publicationDate":"2020","journal":"Elife","firstPage":"e56048","lastPage":"e56048","volume":"9"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS)","FUNCTION","SUBUNIT","INTERACTION WITH REOY AND MURA","PHOSPHORYLATION AT THR-7","DISRUPTION PHENOTYPE","MUTAGENESIS OF THR-7; ARG-57 AND ARG-62"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"6TIF"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL591979","properties":[{"key":"ProteinId","value":"CAC99581.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"AG1262","properties":[{"key":"EntryName","value":"AG1262"}]},{"database":"RefSeq","id":"NP_465028.1","properties":[{"key":"NucleotideSequenceId","value":"NC_003210.1"}]},{"database":"RefSeq","id":"WP_003719797.1","properties":[{"key":"NucleotideSequenceId","value":"NC_003210.1"}]},{"database":"PDB","id":"6TIF","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A/B=1-90"}]},{"database":"PDBsum","id":"6TIF","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P60357","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P60357","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"169963.gene:17594160","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"169963-gene:17594160","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAC99581","properties":[{"key":"ProteinId","value":"CAC99581"},{"key":"GeneId","value":"CAC99581"}]},{"database":"GeneID","id":"86846379","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93239380","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"987746","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"lmo:lmo1503","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|169963.11.peg.1544","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG4472","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_162466_0_0_9","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9796303at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P60357","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"LMON169963:LMO1503-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000817","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"HAMAP","id":"MF_01507","properties":[{"key":"EntryName","value":"IreB_ReoM"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR009309","properties":[{"key":"EntryName","value":"IreB-like"}]},{"database":"NCBIfam","id":"NF003997","properties":[{"key":"EntryName","value":"PRK05473.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR40067","properties":[{"key":"EntryName","value":"UPF0297 PROTEIN YRZL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR40067:SF1","properties":[{"key":"EntryName","value":"UPF0297 PROTEIN YRZL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06135","properties":[{"key":"EntryName","value":"IreB"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF037258","properties":[{"key":"EntryName","value":"DUF965_bac"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDSKDQTMFYNFGDDSIEEDVKKLMKQVYVALEEKGYNPVNQIVGYLLSGDPAYIPRHKDARSMIRRLERDEIIEELVKAYLKNNEIGEK","length":90,"molWeight":10544,"crc64":"D9328F426F62B3BD","md5":"E51AC120F65BB0E70B19933CFBCEE867"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"PTM":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Modified residue":1,"Mutagenesis":3,"Helix":5},"uniParcId":"UPI0000055394"}}