{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P54745","uniProtkbId":"MNGA_ECOLI","entryAudit":{"firstPublicDate":"1996-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-10-01","entryVersion":186,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC component"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme MngA"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"Protein-Npi-phosphohistidine--2-O-alpha-mannosyl-D-glycerate phosphotransferase"}}],"includes":[{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme IIA component"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"PTS system EIIA component"}}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme IIB component"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"}],"value":"2.7.1.195"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"PTS system EIIB component"}}]},{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"2-O-alpha-mannosyl-D-glycerate-specific permease IIC component"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"PTS system EIIC component"}}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14645248"}],"value":"mngA"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9063979"}],"value":"hrsA"}],"orderedLocusNames":[{"value":"b0731"},{"value":"JW0720"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9063979"}],"value":"The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (PubMed:14645248, PubMed:9063979). This system is involved in mannosyl-D-glycerate transport (PubMed:14645248). Also involved in thermoinduction of ompC (PubMed:9063979)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2R)-2-O-(alpha-D-mannosyl)-glycerate(out) + N(pros)-phospho-L-histidyl-[protein] = (2R)-2-O-(6-phospho-alpha-D-mannosyl)-glycerate(in) + L-histidyl-[protein]","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:33307"},{"database":"Rhea","id":"RHEA-COMP:9745"},{"database":"Rhea","id":"RHEA-COMP:9746"},{"database":"ChEBI","id":"CHEBI:29979"},{"database":"ChEBI","id":"CHEBI:57541"},{"database":"ChEBI","id":"CHEBI:60331"},{"database":"ChEBI","id":"CHEBI:64837"}],"ecNumber":"2.7.1.195","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":0.65,"unit":"nmol/min/mg","enzyme":"enzyme for mannosyl-D-glycerate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"}]}],"michaelisConstants":[{"constant":10.0,"unit":"uM","substrate":"mannosyl-D-glycerate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"}]}]}},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P54745","intActId":"EBI-558542"},"interactantTwo":{"uniProtKBAccession":"P0ACA3","geneName":"sspA","intActId":"EBI-558482"},"numberOfExperiments":5,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14645248"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14645248"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"}],"value":"Induced by mannosyl-D-glycerate. Repressed by MngR"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00417"}],"value":"The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00422"}],"value":"The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}],"value":"The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14645248"}],"value":"Cells lacking this gene are unable to transport mannosyl-D-glycerate"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":658,"modifier":"EXACT"}},"description":"PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC component","featureId":"PRO_0000186699"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":314,"modifier":"EXACT"},"end":{"value":334,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":335,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":359,"modifier":"EXACT"},"end":{"value":379,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":389,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":410,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":411,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":434,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":455,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":495,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":500,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":521,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":522,"modifier":"EXACT"},"end":{"value":551,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":552,"modifier":"EXACT"},"end":{"value":572,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":573,"modifier":"EXACT"},"end":{"value":573,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":574,"modifier":"EXACT"},"end":{"value":594,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":595,"modifier":"EXACT"},"end":{"value":620,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":621,"modifier":"EXACT"},"end":{"value":641,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Topological domain","location":{"start":{"value":642,"modifier":"EXACT"},"end":{"value":658,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Domain","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"PTS EIIA type-2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00417"}]},{"type":"Domain","location":{"start":{"value":186,"modifier":"EXACT"},"end":{"value":282,"modifier":"EXACT"}},"description":"PTS EIIB type-2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00422"}]},{"type":"Domain","location":{"start":{"value":306,"modifier":"EXACT"},"end":{"value":641,"modifier":"EXACT"}},"description":"PTS EIIC type-2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00427"}]},{"type":"Active site","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Tele-phosphohistidine intermediate; for EIIA activity","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00417"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9063979"}]},{"type":"Active site","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"Phosphocysteine intermediate; for EIIB activity","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"9063979"}]},{"type":"Modified residue","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Phosphohistidine; by HPr","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Modified residue","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"Phosphocysteine; by EIIA","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00422"},{"evidenceCode":"ECO:0000305"}]}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0598","category":"Biological process","name":"Phosphotransferase system"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0762","category":"Biological process","name":"Sugar transport"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9063979","citationType":"journal article","authors":["Utsumi R.","Horie T.","Katoh A.","Kaino Y.","Tanabe H.","Noda M."],"citationCrossReferences":[{"database":"PubMed","id":"9063979"},{"database":"DOI","id":"10.1271/bbb.60.309"}],"title":"Isolation and characterization of the heat-responsive genes in Escherichia coli.","publicationDate":"1996","journal":"Biosci. Biotechnol. Biochem.","firstPage":"309","lastPage":"315","volume":"60"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","ACTIVE SITE"]},{"referenceNumber":2,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"8905232","citationType":"journal article","authors":["Oshima T.","Aiba H.","Baba T.","Fujita K.","Hayashi K.","Honjo A.","Ikemoto K.","Inada T.","Itoh T.","Kajihara M.","Kanai K.","Kashimoto K.","Kimura S.","Kitagawa M.","Makino K.","Masuda S.","Miki T.","Mizobuchi K.","Mori H.","Motomura K.","Nakamura Y.","Nashimoto H.","Nishio Y.","Saito N.","Sampei G.","Seki Y.","Tagami H.","Takemoto K.","Wada C.","Yamamoto Y.","Yano M.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"8905232"},{"database":"DOI","id":"10.1093/dnares/3.3.137"}],"title":"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.","publicationDate":"1996","journal":"DNA Res.","firstPage":"137","lastPage":"155","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 277-658"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"14645248","citationType":"journal article","authors":["Sampaio M.-M.","Chevance F.","Dippel R.","Eppler T.","Schlegel A.","Boos W.","Lu Y.-J.","Rock C.O."],"citationCrossReferences":[{"database":"PubMed","id":"14645248"},{"database":"DOI","id":"10.1074/jbc.m310980200"}],"title":"Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.","publicationDate":"2004","journal":"J. Biol. Chem.","firstPage":"5537","lastPage":"5548","volume":"279"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","DISRUPTION PHENOTYPE","INDUCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":6,"citation":{"id":"15919996","citationType":"journal article","authors":["Daley D.O.","Rapp M.","Granseth E.","Melen K.","Drew D.","von Heijne G."],"citationCrossReferences":[{"database":"PubMed","id":"15919996"},{"database":"DOI","id":"10.1126/science.1109730"}],"title":"Global topology analysis of the Escherichia coli inner membrane proteome.","publicationDate":"2005","journal":"Science","firstPage":"1321","lastPage":"1323","volume":"308"},"referencePositions":["TOPOLOGY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"D64014","properties":[{"key":"ProteinId","value":"BAA10893.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC73825.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAA35397.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"JC4598","properties":[{"key":"EntryName","value":"JC4598"}]},{"database":"RefSeq","id":"NP_415259.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"AlphaFoldDB","id":"P54745","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P54745","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4263541","properties":[{"key":"Interactions","value":"15"}]},{"database":"BioGRID","id":"849731","properties":[{"key":"Interactions","value":"1"}]},{"database":"DIP","id":"DIP-9939N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P54745","properties":[{"key":"Number of interactors","value":"199"}]},{"database":"IntAct","id":"P54745","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"511145.b0731","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"4.A.2.1.3","properties":[{"key":"FamilyName","value":"the pts fructose-mannitol (fru) family"}]},{"database":"PaxDb","id":"511145-b0731","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC73825","properties":[{"key":"ProteinId","value":"AAC73825"},{"key":"GeneId","value":"b0731"}]},{"database":"GeneID","id":"945355","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW0720","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b0731","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|511145.12.peg.761","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB3024","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1299","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"eggNOG","id":"COG1445","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"eggNOG","id":"COG1762","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_013155_1_2_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P54745","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"QENSWLW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9782569at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P54745","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:HRSA-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:HRSA-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.7.1.195","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"PRO","id":"PR:P54745","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14645248"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15919996"}]},{"database":"GO","id":"GO:0005351","properties":[{"key":"GoTerm","value":"F:carbohydrate:proton symporter activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0022877","properties":[{"key":"GoTerm","value":"F:protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0090581","properties":[{"key":"GoTerm","value":"F:protein-phosphocysteine-mannosylglycerate-phosphotransferase system transporter activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14645248"}]},{"database":"GO","id":"GO:0090563","properties":[{"key":"GoTerm","value":"F:protein-phosphocysteine-sugar phosphotransferase activity"},{"key":"GoEvidenceType","value":"ISM:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PubMed","id":"9063979"}]},{"database":"GO","id":"GO:0051476","properties":[{"key":"GoTerm","value":"P:phosphoenolpyruvate-dependent mannosylglycerate phosphotransferase system"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14645248"}]},{"database":"GO","id":"GO:0009401","properties":[{"key":"GoTerm","value":"P:phosphoenolpyruvate-dependent sugar phosphotransferase system"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14645248"}]},{"database":"CDD","id":"cd00211","properties":[{"key":"EntryName","value":"PTS_IIA_fru"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd05569","properties":[{"key":"EntryName","value":"PTS_IIB_fructose"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.930.10:FF:000025","properties":[{"key":"EntryName","value":"PTS 2-O-a-mannosyl-D-glycerate transporter subunit IIABC"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.2300:FF:000014","properties":[{"key":"EntryName","value":"PTS system fructose-like transporter subunit IIB"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.2300","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.930.10","properties":[{"key":"EntryName","value":"Mannitol-specific EII, Chain A"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR050864","properties":[{"key":"EntryName","value":"Bacterial_PTS_Sugar_Transport"}]},{"database":"InterPro","id":"IPR016152","properties":[{"key":"EntryName","value":"PTrfase/Anion_transptr"}]},{"database":"InterPro","id":"IPR002178","properties":[{"key":"EntryName","value":"PTS_EIIA_type-2_dom"}]},{"database":"InterPro","id":"IPR036095","properties":[{"key":"EntryName","value":"PTS_EIIB-like_sf"}]},{"database":"InterPro","id":"IPR013011","properties":[{"key":"EntryName","value":"PTS_EIIB_2"}]},{"database":"InterPro","id":"IPR003501","properties":[{"key":"EntryName","value":"PTS_EIIB_2/3"}]},{"database":"InterPro","id":"IPR003352","properties":[{"key":"EntryName","value":"PTS_EIIC"}]},{"database":"InterPro","id":"IPR013014","properties":[{"key":"EntryName","value":"PTS_EIIC_2"}]},{"database":"InterPro","id":"IPR004715","properties":[{"key":"EntryName","value":"PTS_IIA_fruc"}]},{"database":"InterPro","id":"IPR003353","properties":[{"key":"EntryName","value":"PTS_IIB_fruc"}]},{"database":"InterPro","id":"IPR006327","properties":[{"key":"EntryName","value":"PTS_IIC_fruc"}]},{"database":"NCBIfam","id":"TIGR00829","properties":[{"key":"EntryName","value":"FRU"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00848","properties":[{"key":"EntryName","value":"fruA"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF007293","properties":[{"key":"EntryName","value":"PRK09765.1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR01427","properties":[{"key":"EntryName","value":"PTS_IIC_fructo"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30505","properties":[{"key":"EntryName","value":"FRUCTOSE-LIKE PERMEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30505:SF28","properties":[{"key":"EntryName","value":"PTS SYSTEM 2-O-ALPHA-MANNOSYL-D-GLYCERATE-SPECIFIC EIIABC COMPONENT"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00359","properties":[{"key":"EntryName","value":"PTS_EIIA_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02378","properties":[{"key":"EntryName","value":"PTS_EIIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02302","properties":[{"key":"EntryName","value":"PTS_IIB"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55804","properties":[{"key":"EntryName","value":"Phoshotransferase/anion transport protein"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52794","properties":[{"key":"EntryName","value":"PTS system IIB component-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51094","properties":[{"key":"EntryName","value":"PTS_EIIA_TYPE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00372","properties":[{"key":"EntryName","value":"PTS_EIIA_TYPE_2_HIS"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51099","properties":[{"key":"EntryName","value":"PTS_EIIB_TYPE_2"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51104","properties":[{"key":"EntryName","value":"PTS_EIIC_TYPE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQFLEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAGYLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRHAVKHGNYLTDGVMP","length":658,"molWeight":69668,"crc64":"C1F0999A1C48C5C2","md5":"9630067511AC7A773B5B05692521945A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DOMAIN":3,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Topological domain":10,"Transmembrane":9,"Domain":3,"Active site":2,"Modified residue":2},"uniParcId":"UPI0000047CA4"}}