{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P53843","secondaryAccessions":["D6W0S8"],"uniProtkbId":"IST1_YEAST","entryAudit":{"firstPublicDate":"1996-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-10-01","entryVersion":173,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Vacuolar protein sorting-associated protein IST1"}},"alternativeNames":[{"fullName":{"value":"Increased sodium tolerance protein 1"}}]},"genes":[{"geneName":{"value":"IST1"},"orderedLocusNames":[{"value":"YNL265C"}],"orfNames":[{"value":"N0809"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18032582"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18032584"}],"value":"Involved in a late step in sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins are targeted into the vacuole after fusion of the endosome with the vacuole. Regulates the recruitment of VPS4 to the ESCRT-III complex, probably in conjunction with DID2, and VPS4 catalyzes the disassembly of the ESCRT-III complex"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18032582"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18032584"}],"value":"Interacts with DID2. Interacts with VPS4 (via MIT domain); the interaction prevents VPS4 oligomerization, competes with the binding of VTA1 to VSP4 and diminishes the ATPase activity of VSP4"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P53843","intActId":"EBI-28245"},"interactantTwo":{"uniProtKBAccession":"P69771","geneName":"DID2","intActId":"EBI-2053489"},"numberOfExperiments":4,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"The endosomal location appears to be dependent on DID2"}]},"subcellularLocations":[{"location":{"value":"Cytoplasm","id":"SL-0086"}},{"location":{"value":"Endosome","id":"SL-0101"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 4760 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the IST1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"Vacuolar protein sorting-associated protein IST1","featureId":"PRO_0000076227"},{"type":"Region","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":298,"modifier":"EXACT"}},"description":"Interaction with VPS4"},{"type":"Motif","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"MIT-interacting motif"},{"type":"Compositional bias","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Helix","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Beta strand","location":{"start":{"value":100,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]},{"type":"Helix","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":188,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3GGY"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"8740425","citationType":"journal article","authors":["Sen-Gupta M.","Lyck R.","Fleig U.","Niedenthal R.K.","Hegemann J.H."],"citationCrossReferences":[{"database":"PubMed","id":"8740425"},{"database":"DOI","id":"10.1002/(sici)1097-0061(199604)12:5%3c505::aid-yea932%3e3.0.co;2-f"}],"title":"The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes.","publicationDate":"1996","journal":"Yeast","firstPage":"505","lastPage":"514","volume":"12"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 96604 / S288c / FY1679","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9169873","citationType":"journal article","authors":["Philippsen P.","Kleine K.","Poehlmann R.","Duesterhoeft A.","Hamberg K.","Hegemann J.H.","Obermaier B.","Urrestarazu L.A.","Aert R.","Albermann K.","Altmann R.","Andre B.","Baladron V.","Ballesta J.P.G.","Becam A.-M.","Beinhauer J.D.","Boskovic J.","Buitrago M.J.","Bussereau F.","Coster F.","Crouzet M.","D'Angelo M.","Dal Pero F.","De Antoni A.","del Rey F.","Doignon F.","Domdey H.","Dubois E.","Fiedler T.A.","Fleig U.","Floeth M.","Fritz C.","Gaillardin C.","Garcia-Cantalejo J.M.","Glansdorff N.","Goffeau A.","Gueldener U.","Herbert C.J.","Heumann K.","Heuss-Neitzel D.","Hilbert H.","Hinni K.","Iraqui Houssaini I.","Jacquet M.","Jimenez A.","Jonniaux J.-L.","Karpfinger-Hartl L.","Lanfranchi G.","Lepingle A.","Levesque H.","Lyck R.","Maftahi M.","Mallet L.","Maurer C.T.C.","Messenguy F.","Mewes H.-W.","Moestl D.","Nasr F.","Nicaud J.-M.","Niedenthal R.K.","Pandolfo D.","Pierard A.","Piravandi E.","Planta R.J.","Pohl T.M.","Purnelle B.","Rebischung C.","Remacha M.A.","Revuelta J.L.","Rinke M.","Saiz J.E.","Sartorello F.","Scherens B.","Sen-Gupta M.","Soler-Mira A.","Urbanus J.H.M.","Valle G.","Van Dyck L.","Verhasselt P.","Vierendeels F.","Vissers S.","Voet M.","Volckaert G.","Wach A.","Wambutt R.","Wedler H.","Zollner A.","Hani J."],"citationCrossReferences":[{"database":"PubMed","id":"9169873"},{"database":"DOI","id":"10.1038/387s093"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.","publicationDate":"1997","journal":"Nature","firstPage":"93","lastPage":"98","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":5,"citation":{"id":"18032582","citationType":"journal article","authors":["Dimaano C.","Jones C.B.","Hanono A.","Curtiss M.","Babst M."],"citationCrossReferences":[{"database":"PubMed","id":"18032582"},{"database":"DOI","id":"10.1091/mbc.e07-08-0747"}],"title":"Ist1 regulates Vps4 localization and assembly.","publicationDate":"2008","journal":"Mol. Biol. Cell","firstPage":"465","lastPage":"474","volume":"19"},"referencePositions":["FUNCTION","INTERACTION WITH VPS4","SUBCELLULAR LOCATION"]},{"referenceNumber":6,"citation":{"id":"18032584","citationType":"journal article","authors":["Rue S.M.","Mattei S.","Saksena S.","Emr S.D."],"citationCrossReferences":[{"database":"PubMed","id":"18032584"},{"database":"DOI","id":"10.1091/mbc.e07-07-0694"}],"title":"Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting.","publicationDate":"2008","journal":"Mol. Biol. Cell","firstPage":"475","lastPage":"484","volume":"19"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH DID2 AND VPS4"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X92494","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z71542","properties":[{"key":"ProteinId","value":"CAA96172.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006947","properties":[{"key":"ProteinId","value":"DAA10294.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S63238","properties":[{"key":"EntryName","value":"S63238"}]},{"database":"RefSeq","id":"NP_014134.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001183103.1"}]},{"database":"PDB","id":"3GGY","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"A/B=1-193"}]},{"database":"PDB","id":"3GGZ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.80 A"},{"key":"Chains","value":"A/B/C/D=1-193"}]},{"database":"PDBsum","id":"3GGY","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3GGZ","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"35574","properties":[{"key":"Interactions","value":"72"}]},{"database":"DIP","id":"DIP-6713N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P53843","properties":[{"key":"Number of interactors","value":"903"}]},{"database":"IntAct","id":"P53843","properties":[{"key":"Interactions","value":"31"}]},{"database":"MINT","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YNL265C","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.A.31.1.1","properties":[{"key":"FamilyName","value":"the endosomal sorting complexes required for transport iii (escrt-iii) family"}]},{"database":"iPTMnet","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YNL265C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YNL265C_mRNA","properties":[{"key":"ProteinId","value":"YNL265C"},{"key":"GeneId","value":"YNL265C"}]},{"database":"GeneID","id":"855456","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YNL265C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000005209","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000005209","properties":[{"key":"GeneName","value":"IST1"}]},{"database":"VEuPathDB","id":"FungiDB:YNL265C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2027","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_037652_2_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"HEVREFT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"29853at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-33261-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-SCE-6798695","properties":[{"key":"PathwayName","value":"Neutrophil degranulation"}]},{"database":"Reactome","id":"R-SCE-9668328","properties":[{"key":"PathwayName","value":"Sealing of the nuclear envelope (NE) by ESCRT-III"}]},{"database":"BioGRID-ORCS","id":"855456","properties":[{"key":"hits","value":"1 hit in 10 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"P53843","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P53843","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome XIV"}]},{"database":"RNAct","id":"P53843","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18032584"}]},{"database":"GO","id":"GO:0005768","properties":[{"key":"GoTerm","value":"C:endosome"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18032584"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36125415"}]},{"database":"GO","id":"GO:0042030","properties":[{"key":"GoTerm","value":"F:ATPase inhibitor activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18032582"}]},{"database":"GO","id":"GO:0099638","properties":[{"key":"GoTerm","value":"P:endosome to plasma membrane protein transport"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"36125415"}]},{"database":"GO","id":"GO:0008104","properties":[{"key":"GoTerm","value":"P:intracellular protein localization"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0032511","properties":[{"key":"GoTerm","value":"P:late endosome to vacuole transport via multivesicular body sorting pathway"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"18032582"}]},{"database":"FunFam","id":"1.20.1260.60:FF:000002","properties":[{"key":"EntryName","value":"Vacuolar protein sorting-associated protein IST1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.1260.60","properties":[{"key":"EntryName","value":"Vacuolar protein sorting-associated protein Ist1"},{"key":"MatchStatus","value":"1"}]},{"database":"IDEAL","id":"IID50374","properties":[{"key":"Description","value":"-"}]},{"database":"InterPro","id":"IPR005061","properties":[{"key":"EntryName","value":"Ist1"}]},{"database":"InterPro","id":"IPR042277","properties":[{"key":"EntryName","value":"IST1-like"}]},{"database":"PANTHER","id":"PTHR12161","properties":[{"key":"EntryName","value":"IST1 FAMILY MEMBER"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12161:SF5","properties":[{"key":"EntryName","value":"IST1 HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03398","properties":[{"key":"EntryName","value":"Ist1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAPSMIPFTIKLKTCLKMCIQRLRYAQEKQQAIAKQSRRQVAQLLLTNKEQKAHYRVETLIHDDIHIELLEILELYCELLLARVQVINDISTEEQLVKEHMDDGINEAIRSLIYAILFVDEVKELSQLKDLMAWKINVEFVNGVIADHIDVPEKIIKKCSPSVPKEELVDLYLKEIAKTYDVPYSKLENSLSSSSSNISSDFSDPSGDIEDNDEEKPILALDNDDNDNADAKHPITVKKPRQNSENIKNELKIPKDIKKEVIEKKQSEKKTTKRKTKKEQENDELDELKKRFDALRRK","length":298,"molWeight":34496,"crc64":"F16A94BFC7716A1A","md5":"08D2B6CF82BAD7EBD3F60FBAACB5B250"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":2,"Motif":1,"Compositional bias":2,"Helix":12,"Beta strand":1},"uniParcId":"UPI00000530CC"}}