{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P53081","secondaryAccessions":["D6VVB4","E9P932"],"uniProtkbId":"NIF3_YEAST","entryAudit":{"firstPublicDate":"1996-10-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1996-10-01","entryVersion":167,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"8663102"}],"value":"NGG1-interacting factor 3"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"SGD","id":"S000003189"}],"value":"NIF3"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"SGD","id":"S000003189"}],"value":"YGL221C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8663102"}],"value":"May interact with NGG1"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16823961"}],"value":"Mitochondrion","id":"SL-0173"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 6000 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DUF34/NIF3 family. NIF3/YbgI subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":288,"modifier":"EXACT"}},"description":"NGG1-interacting factor 3","featureId":"PRO_0000147356"},{"type":"Sequence conflict","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"in Ref. 4; AAT93235","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9290212","citationType":"journal article","authors":["Rieger M.","Brueckner M.","Schaefer M.","Mueller-Auer S."],"citationCrossReferences":[{"database":"PubMed","id":"9290212"},{"database":"DOI","id":"10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y"}],"title":"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII.","publicationDate":"1997","journal":"Yeast","firstPage":"1077","lastPage":"1090","volume":"13"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9169869","citationType":"journal article","authors":["Tettelin H.","Agostoni-Carbone M.L.","Albermann K.","Albers M.","Arroyo J.","Backes U.","Barreiros T.","Bertani I.","Bjourson A.J.","Brueckner M.","Bruschi C.V.","Carignani G.","Castagnoli L.","Cerdan E.","Clemente M.L.","Coblenz A.","Coglievina M.","Coissac E.","Defoor E.","Del Bino S.","Delius H.","Delneri D.","de Wergifosse P.","Dujon B.","Durand P.","Entian K.-D.","Eraso P.","Escribano V.","Fabiani L.","Fartmann B.","Feroli F.","Feuermann M.","Frontali L.","Garcia-Gonzalez M.","Garcia-Saez M.I.","Goffeau A.","Guerreiro P.","Hani J.","Hansen M.","Hebling U.","Hernandez K.","Heumann K.","Hilger F.","Hofmann B.","Indge K.J.","James C.M.","Klima R.","Koetter P.","Kramer B.","Kramer W.","Lauquin G.","Leuther H.","Louis E.J.","Maillier E.","Marconi A.","Martegani E.","Mazon M.J.","Mazzoni C.","McReynolds A.D.K.","Melchioretto P.","Mewes H.-W.","Minenkova O.","Mueller-Auer S.","Nawrocki A.","Netter P.","Neu R.","Nombela C.","Oliver S.G.","Panzeri L.","Paoluzi S.","Plevani P.","Portetelle D.","Portillo F.","Potier S.","Purnelle B.","Rieger M.","Riles L.","Rinaldi T.","Robben J.","Rodrigues-Pousada C.","Rodriguez-Belmonte E.","Rodriguez-Torres A.M.","Rose M.","Ruzzi M.","Saliola M.","Sanchez-Perez M.","Schaefer B.","Schaefer M.","Scharfe M.","Schmidheini T.","Schreer A.","Skala J.","Souciet J.-L.","Steensma H.Y.","Talla E.","Thierry A.","Vandenbol M.","van der Aart Q.J.M.","Van Dyck L.","Vanoni M.","Verhasselt P.","Voet M.","Volckaert G.","Wambutt R.","Watson M.D.","Weber N.","Wedler E.","Wedler H.","Wipfli P.","Wolf K.","Wright L.F.","Zaccaria P.","Zimmermann M.","Zollner A.","Kleine K."],"citationCrossReferences":[{"database":"PubMed","id":"9169869"},{"database":"DOI","id":"10.1038/387s081"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.","publicationDate":"1997","journal":"Nature","firstPage":"81","lastPage":"84","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"17322287","citationType":"journal article","authors":["Hu Y.","Rolfs A.","Bhullar B.","Murthy T.V.S.","Zhu C.","Berger M.F.","Camargo A.A.","Kelley F.","McCarron S.","Jepson D.","Richardson A.","Raphael J.","Moreira D.","Taycher E.","Zuo D.","Mohr S.","Kane M.F.","Williamson J.","Simpson A.J.G.","Bulyk M.L.","Harlow E.","Marsischky G.","Kolodner R.D.","LaBaer J."],"citationCrossReferences":[{"database":"PubMed","id":"17322287"},{"database":"DOI","id":"10.1101/gr.6037607"}],"title":"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.","publicationDate":"2007","journal":"Genome Res.","firstPage":"536","lastPage":"543","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"8663102","citationType":"journal article","authors":["Martens J.A.","Genereaux J.","Saleh A.","Brandl C.J."],"citationCrossReferences":[{"database":"PubMed","id":"8663102"},{"database":"DOI","id":"10.1074/jbc.271.27.15884"}],"title":"Transcriptional activation by yeast PDR1p is inhibited by its association with NGG1p/ADA3p.","publicationDate":"1996","journal":"J. Biol. Chem.","firstPage":"15884","lastPage":"15890","volume":"271"},"referencePositions":["INTERACTION WITH NGG1"]},{"referenceNumber":6,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":7,"citation":{"id":"16823961","citationType":"journal article","authors":["Reinders J.","Zahedi R.P.","Pfanner N.","Meisinger C.","Sickmann A."],"citationCrossReferences":[{"database":"PubMed","id":"16823961"},{"database":"DOI","id":"10.1021/pr050477f"}],"title":"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.","publicationDate":"2006","journal":"J. Proteome Res.","firstPage":"1543","lastPage":"1554","volume":"5"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]","IDENTIFICATION BY MASS SPECTROMETRY"]},{"referenceNumber":8,"citation":{"id":"18407956","citationType":"journal article","authors":["Albuquerque C.P.","Smolka M.B.","Payne S.H.","Bafna V.","Eng J.","Zhou H."],"citationCrossReferences":[{"database":"PubMed","id":"18407956"},{"database":"DOI","id":"10.1074/mcp.m700468-mcp200"}],"title":"A multidimensional chromatography technology for in-depth phosphoproteome analysis.","publicationDate":"2008","journal":"Mol. Cell. Proteomics","firstPage":"1389","lastPage":"1396","volume":"7"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z72743","properties":[{"key":"ProteinId","value":"CAA96937.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY693216","properties":[{"key":"ProteinId","value":"AAT93235.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006941","properties":[{"key":"ProteinId","value":"DAA07898.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S64243","properties":[{"key":"EntryName","value":"S64243"}]},{"database":"RefSeq","id":"NP_011294.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001181086.1"}]},{"database":"AlphaFoldDB","id":"P53081","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P53081","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"33038","properties":[{"key":"Interactions","value":"41"}]},{"database":"DIP","id":"DIP-1469N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P53081","properties":[{"key":"Number of interactors","value":"723"}]},{"database":"IntAct","id":"P53081","properties":[{"key":"Interactions","value":"19"}]},{"database":"MINT","id":"P53081","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YGL221C","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P53081","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YGL221C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P53081","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YGL221C_mRNA","properties":[{"key":"ProteinId","value":"YGL221C"},{"key":"GeneId","value":"YGL221C"}]},{"database":"GeneID","id":"852651","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YGL221C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000003189","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000003189","properties":[{"key":"GeneName","value":"NIF3"}]},{"database":"VEuPathDB","id":"FungiDB:YGL221C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG4131","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_037423_0_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P53081","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"NFDKTHL","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"3345469at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-30695-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"852651","properties":[{"key":"hits","value":"8 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:P53081","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome VII"}]},{"database":"RNAct","id":"P53081","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17034789"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17034789"}]},{"database":"FunFam","id":"3.40.1390.30:FF:000001","properties":[{"key":"EntryName","value":"GTP cyclohydrolase 1 type 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1390.30","properties":[{"key":"EntryName","value":"NIF3 (NGG1p interacting factor 3)-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002678","properties":[{"key":"EntryName","value":"DUF34/NIF3"}]},{"database":"InterPro","id":"IPR036069","properties":[{"key":"EntryName","value":"DUF34/NIF3_sf"}]},{"database":"NCBIfam","id":"TIGR00486","properties":[{"key":"EntryName","value":"YbgI_SA1388"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13799","properties":[{"key":"EntryName","value":"NGG1 INTERACTING FACTOR 3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13799:SF13","properties":[{"key":"EntryName","value":"NIF3-LIKE PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01784","properties":[{"key":"EntryName","value":"DUF34_NIF3"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102705","properties":[{"key":"EntryName","value":"NIF3 (NGG1p interacting factor 3)-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSRAITRAQLDKLVRSITKFYPQKYADKSWDNTGLLIDCSTAQVTTADANAKTKVLLTVDLTKSVAQEAVDANCNVIVAYHPFIFPSWNRLSPHTNPQHETAIKLIQYGISVYCPHTAVDAARGGVNDWLVRGLNNGENVAKSYALETVSGETDDLIGYGRFVEFNKDISLEQIVKNVKRVLRVPYVQVASLAAPSAWNQLKIKKVAVCAGSGSGVFKQLKEDVDLYYTGEMSHHEVLKWKEMGKTVIVCNHSNTERGFLQDVMKGLLQDEGHEVVVSKMDCDPLTVA","length":288,"molWeight":31888,"crc64":"B6AB6E48AAF4776A","md5":"4AB8556C1A1C4CCF50627E6DA75E791F"},"extraAttributes":{"countByCommentType":{"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Sequence conflict":1},"uniParcId":"UPI000013007D"}}