{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P52283","uniProtkbId":"T2C1_PBCVI","entryAudit":{"firstPublicDate":"1996-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2022-02-23","entryVersion":57,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Paramecium bursaria Chlorella virus IL3A","commonName":"PBCV-IL3A","taxonId":46019,"lineage":["Viruses","Varidnaviria","Bamfordvirae","Nucleocytoviricota","Megaviricetes","Algavirales","Phycodnaviridae","Chlorovirus","Chlorovirus vanettense","Chlorovirus illinoense"]},"organismHosts":[{"scientificName":"Chlorella","taxonId":3071}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12654995"}],"value":"Type II restriction enzyme CviJI"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"7607522"}],"value":"R.CviJI"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8692682"}],"value":"3.1.21.4"}]},"alternativeNames":[{"fullName":{"value":"Endonuclease CviJI"}},{"fullName":{"value":"Type-2 restriction enzyme CviJI"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"7607522"}],"value":"CVJIR"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8692682"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12654995"}],"value":"A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-RGCY-3' and cleaves after G-2. In the presence of ATP, there is a relaxation of its specificity and it can cleave 5'-RGCN-3' and 5'-YGCY-3', but not 5'-YGCR-3' (R.CviJI* activity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.","ecNumber":"3.1.21.4","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8692682"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8692682"}],"value":"It is uncertain whether Met-1 or Val-81 is the initiator; upon expression in E.coli the protein is 32.5 kDa and starts at Val-81. Recombinant enzyme has no R.CviJI* activity, whereas viral-produced enzyme is larger than 32.5 kDa and does have R.CviJI* activity, suggesting Met-1 is the correct initiator when grown in Chlorella strain NC64A"}],"commentType":"CAUTION"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAC55064.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":358,"modifier":"EXACT"}},"description":"Type II restriction enzyme CviJI","featureId":"PRO_0000077298"}],"keywords":[{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0680","category":"Biological process","name":"Restriction system"}],"references":[{"referenceNumber":1,"citation":{"id":"7607522","citationType":"journal article","authors":["Skowron P.M.","Swaminathan N.","McMaster K.","George D.","van Etten J.L.","Mead D.A."],"citationCrossReferences":[{"database":"PubMed","id":"7607522"},{"database":"DOI","id":"10.1016/0378-1119(94)00564-9"}],"title":"Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella.","publicationDate":"1995","journal":"Gene","firstPage":"37","lastPage":"41","volume":"157"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"8692682","citationType":"journal article","authors":["Swaminathan N.","Mead D.A.","McMaster K.","George D.","van Etten J.L.","Skowron 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V.","Piekarowicz A.","Pingoud A.","Raleigh E.","Rao D.N.","Reich N.","Repin V.E.","Selker E.U.","Shaw P.C.","Stein D.C.","Stoddard B.L.","Szybalski W.","Trautner T.A.","Van Etten J.L.","Vitor J.M.","Wilson G.G.","Xu S.Y."],"citationCrossReferences":[{"database":"PubMed","id":"12654995"},{"database":"DOI","id":"10.1093/nar/gkg274"}],"title":"A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.","publicationDate":"2003","journal":"Nucleic Acids 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