{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P49857","secondaryAccessions":["O34690"],"uniProtkbId":"GDND_BACSU","entryAudit":{"firstPublicDate":"1996-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2000-05-30","entryVersion":141,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Probable guanidinium efflux system subunit GdnD"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAB13167.1"}],"value":"gdnD"},"synonyms":[{"value":"ykkD"}],"orderedLocusNames":[{"value":"BSU13100"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10735877"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27989440"}],"value":"Probably involved in guanidinium transport (PubMed:27989440). In vitro, confers resistance to a broad range of toxic compounds such as cationic dyes, neutral and anionic antimicrobials (PubMed:10735877)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"10735877"}],"value":"The efflux pump is composed of GdnC and GdnD"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27989440"}],"value":"Transcriptionally regulated by guanidine, via a guanidine-sensing riboswitch"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27989440"}],"value":"Deletion of both gdnC and gdnD leads to a decrease in the concentration of guanidine required to inhibit bacterial growth"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. YkkC/YkkD subfamily"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"BAA11263.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"Probable guanidinium efflux system subunit GdnD","featureId":"PRO_0000108113"},{"type":"Transmembrane","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"8682784","citationType":"journal article","authors":["Lacelle M.","Kumano M.","Kurita K.","Yamane K.","Zuber P.","Nakano M.M."],"citationCrossReferences":[{"database":"PubMed","id":"8682784"},{"database":"DOI","id":"10.1128/jb.178.13.3803-3808.1996"}],"title":"Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus subtilis.","publicationDate":"1996","journal":"J. Bacteriol.","firstPage":"3803","lastPage":"3808","volume":"178"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"CI-6IV0A1CQJFG2P","citationType":"submission","authors":["Devine K.M."],"title":"Sequence of the Bacillus subtilis genome between xlyA and ykoR.","publicationDate":"NOV-1997","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"10735877","citationType":"journal article","authors":["Jack D.L.","Storms M.L.","Tchieu J.H.","Paulsen I.T.","Saier M.H. Jr."],"citationCrossReferences":[{"database":"PubMed","id":"10735877"},{"database":"DOI","id":"10.1128/jb.182.8.2311-2313.2000"}],"title":"A broad-specificity multidrug efflux pump requiring a pair of homologous SMR-type proteins.","publicationDate":"2000","journal":"J. Bacteriol.","firstPage":"2311","lastPage":"2313","volume":"182"},"referencePositions":["FUNCTION","SUBUNIT"]},{"referenceNumber":5,"citation":{"id":"27989440","citationType":"journal article","authors":["Nelson J.W.","Atilho R.M.","Sherlock M.E.","Stockbridge R.B.","Breaker R.R."],"citationCrossReferences":[{"database":"PubMed","id":"27989440"},{"database":"DOI","id":"10.1016/j.molcel.2016.11.019"}],"title":"Metabolism of free guanidine in bacteria is regulated by a widespread riboswitch class.","publicationDate":"2017","journal":"Mol. Cell","firstPage":"220","lastPage":"230","volume":"65"},"referencePositions":["FUNCTION","INDUCTION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"D78189","properties":[{"key":"ProteinId","value":"BAA11263.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AJ002571","properties":[{"key":"ProteinId","value":"CAA05589.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB13167.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B69857","properties":[{"key":"EntryName","value":"B69857"}]},{"database":"RefSeq","id":"NP_389193.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_003245695.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"P49857","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P49857","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P49857","properties":[{"key":"Number of interactors","value":"158"}]},{"database":"STRING","id":"224308.BSU13100","properties":[{"key":"Description","value":"-"}]},{"database":"CARD","id":"ARO:3003064","properties":[{"key":"CARD short name","value":"ykkD"},{"key":"Resistance mechanism identifier","value":"ARO:0010000"},{"key":"Resistance mechanism name","value":"antibiotic efflux"}]},{"database":"TCDB","id":"2.A.7.1.5","properties":[{"key":"FamilyName","value":"the drug/metabolite transporter (dmt) superfamily"}]},{"database":"PaxDb","id":"224308-BSU13100","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"938109","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB13167","properties":[{"key":"ProteinId","value":"CAB13167"},{"key":"GeneId","value":"BSU_13100"}]},{"database":"GeneID","id":"938109","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU13100","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.43.peg.1381","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG2076","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"P49857","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"21828at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P49857","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU13100-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P49857","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0022857","properties":[{"key":"GoTerm","value":"F:transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0055085","properties":[{"key":"GoTerm","value":"P:transmembrane transport"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"FunFam","id":"1.10.3730.20:FF:000001","properties":[{"key":"EntryName","value":"Quaternary ammonium compound resistance transporter SugE"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.3730.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR037185","properties":[{"key":"EntryName","value":"EmrE-like"}]},{"database":"InterPro","id":"IPR000390","properties":[{"key":"EntryName","value":"Small_drug/metabolite_transptr"}]},{"database":"InterPro","id":"IPR045324","properties":[{"key":"EntryName","value":"Small_multidrug_res"}]},{"database":"PANTHER","id":"PTHR30561:SF0","properties":[{"key":"EntryName","value":"GUANIDINIUM EXPORTER"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30561","properties":[{"key":"EntryName","value":"SMR FAMILY PROTON-DEPENDENT DRUG EFFLUX TRANSPORTER SUGE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00893","properties":[{"key":"EntryName","value":"Multi_Drug_Res"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103481","properties":[{"key":"EntryName","value":"Multidrug resistance efflux transporter EmrE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLHWISLLCAGCLEMAGVALMNQYAKEKSVKWVLLIIVGFAASFSLLSYAMETTPMGTAYAVWTGIGTAGGALIGILFYKEQKDAKRIFFIALILCSAVGLKILS","length":105,"molWeight":11325,"crc64":"796C69A20142748C","md5":"15FEC150E64FEDE2916C473EB4D5B068"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Transmembrane":4},"uniParcId":"UPI00000602AE"}}