{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P49194","secondaryAccessions":["Q6B4R6","Q8VD34","Q9R0H8"],"uniProtkbId":"RET3_MOUSE","entryAudit":{"firstPublicDate":"1996-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-10-25","entryVersion":163,"sequenceVersion":3},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Retinol-binding protein 3"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P10745"}],"value":"Interphotoreceptor retinoid-binding protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12434304"}],"value":"IRBP"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P10745"}],"value":"Interstitial retinol-binding protein"}}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:97878"}],"value":"Rbp3"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29109151"}],"value":"Mediates the transport of 11-cis- and all-trans-retinoids between the retinal pigment epithelium (RPE) and photoreceptors, facilitating retinoid exchange required for the visual cycle"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Interphotoreceptor matrix that permeates the space between the retina and the contiguous layer of pigment epithelium cells"}]},"subcellularLocations":[{"location":{"value":"Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix","id":"SL-0146"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"1342928"}],"value":"Expressed in the photosensitive tissues; retina and pineal gland"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29109151"}],"value":"Knockout mice display photoreceptor degeneration and disturbed retinoid dynamics. Following photobleach retinas have persistently elevated all-trans-retinol during recovery and reduced all-trans-retinyl esters in the RPE"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase S41A family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":1234,"modifier":"EXACT"}},"description":"Retinol-binding protein 3","featureId":"PRO_0000021524"},{"type":"Repeat","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":320,"modifier":"EXACT"}},"description":"1"},{"type":"Repeat","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":628,"modifier":"EXACT"}},"description":"2"},{"type":"Repeat","location":{"start":{"value":629,"modifier":"EXACT"},"end":{"value":929,"modifier":"EXACT"}},"description":"3"},{"type":"Repeat","location":{"start":{"value":930,"modifier":"EXACT"},"end":{"value":1228,"modifier":"EXACT"}},"description":"4"},{"type":"Region","location":{"start":{"value":18,"modifier":"EXACT"},"end":{"value":1228,"modifier":"EXACT"}},"description":"4 X approximate tandem repeats"},{"type":"Region","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":417,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Glycosylation","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Glycosylation","location":{"start":{"value":513,"modifier":"EXACT"},"end":{"value":513,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Sequence conflict","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"in Ref. 2 and 3","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":770,"modifier":"EXACT"},"end":{"value":773,"modifier":"EXACT"}},"description":"in Ref. 1; AAT81142","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"LVDT","alternativeSequences":["AGGQ"]}},{"type":"Sequence conflict","location":{"start":{"value":930,"modifier":"EXACT"},"end":{"value":930,"modifier":"EXACT"}},"description":"in Ref. 1; AAT81142","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0272","category":"Cellular component","name":"Extracellular matrix"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0845","category":"Ligand","name":"Vitamin A"}],"references":[{"referenceNumber":1,"citation":{"id":"12434304","citationType":"journal article","authors":["Shuler R.K. Jr.","Gross E.","He W.-Y.","Liou G.I.","Nickerson J.M."],"citationCrossReferences":[{"database":"PubMed","id":"12434304"},{"database":"DOI","id":"10.1076/ceyr.24.5.354.8518"}],"title":"Sequence analysis of the mouse IRBP gene and cDNA.","publicationDate":"2002","journal":"Curr. Eye Res.","firstPage":"354","lastPage":"367","volume":"24"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"129/Sv","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Eye","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"CI-CLFQ7211IM1VC","citationType":"submission","authors":["Si J.-S.","Nickerson J.M."],"publicationDate":"APR-1999","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-610"],"referenceComments":[{"value":"BALB/cJ","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"1342928","citationType":"journal article","authors":["Stanhope M.J.","Czelusniak J.","Si J.-S.","Nickerson J.M.","Goodman M."],"citationCrossReferences":[{"database":"PubMed","id":"1342928"},{"database":"DOI","id":"10.1016/1055-7903(92)90026-d"}],"title":"A molecular perspective on mammalian evolution from the gene encoding interphotoreceptor retinoid binding protein, with convincing evidence for bat monophyly.","publicationDate":"1992","journal":"Mol. Phylogenet. Evol.","firstPage":"148","lastPage":"160","volume":"1"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 42-454","TISSUE SPECIFICITY"],"referenceComments":[{"value":"BALB/cJ","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-9U8TVH33OVQA4","citationType":"submission","authors":["Suzuki H.","Serizawa K."],"publicationDate":"OCT-1999","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-454"],"referenceComments":[{"value":"MOA","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"29109151","citationType":"journal article","authors":["Cook J.D.","Ng S.Y.","Lloyd M.","Eddington S.","Sun H.","Nathans J.","Bok D.","Radu R.A.","Travis G.H."],"citationCrossReferences":[{"database":"PubMed","id":"29109151"},{"database":"DOI","id":"10.1074/jbc.m117.812701"}],"title":"Peropsin modulates transit of vitamin A from retina to retinal pigment epithelium.","publicationDate":"2017","journal":"J. Biol. Chem.","firstPage":"21407","lastPage":"21416","volume":"292"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY682090","properties":[{"key":"ProteinId","value":"AAT81142.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC017610","properties":[{"key":"ProteinId","value":"AAH17610.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC042480","properties":[{"key":"ProteinId","value":"AAH42480.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC083133","properties":[{"key":"ProteinId","value":"AAH83133.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF126968","properties":[{"key":"ProteinId","value":"AAA39331.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AB033711","properties":[{"key":"ProteinId","value":"BAA85872.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"CCDS","id":"CCDS26929.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_056560.1","properties":[{"key":"NucleotideSequenceId","value":"NM_015745.3"}]},{"database":"AlphaFoldDB","id":"P49194","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P49194","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"202829","properties":[{"key":"Interactions","value":"2"}]},{"database":"FunCoup","id":"P49194","properties":[{"key":"Number of interactors","value":"125"}]},{"database":"STRING","id":"10090.ENSMUSP00000040249","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"S41.952","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"S41.953","properties":[{"key":"Description","value":"-"}]},{"database":"GlyCosmos","id":"P49194","properties":[{"key":"glycosylation","value":"3 sites, No reported glycans"}]},{"database":"GlyGen","id":"P49194","properties":[{"key":"glycosylation","value":"6 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"P49194","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"P49194","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000040249","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"255288","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"19661","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"19661","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:19661","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc007tad.1","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:97878","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"5949","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:97878","properties":[{"key":"GeneName","value":"Rbp3"}]},{"database":"eggNOG","id":"ENOG502QW81","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"P49194","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"10268064at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P49194","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-2187335","properties":[{"key":"PathwayName","value":"The retinoid cycle in cones (daylight vision)"}]},{"database":"Reactome","id":"R-MMU-2453902","properties":[{"key":"PathwayName","value":"The canonical retinoid cycle in rods (twilight vision)"}]},{"database":"BioGRID-ORCS","id":"19661","properties":[{"key":"hits","value":"4 hits in 82 CRISPR screens"}]},{"database":"PRO","id":"PR:P49194","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Unplaced"}]},{"database":"RNAct","id":"P49194","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0090658","properties":[{"key":"GoTerm","value":"C:cone matrix sheath"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0033165","properties":[{"key":"GoTerm","value":"C:interphotoreceptor matrix"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0019841","properties":[{"key":"GoTerm","value":"F:retinol binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0008236","properties":[{"key":"GoTerm","value":"F:serine-type peptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd07563","properties":[{"key":"EntryName","value":"Peptidase_S41_IRBP"},{"key":"MatchStatus","value":"4"}]},{"database":"FunFam","id":"3.90.226.10:FF:000031","properties":[{"key":"EntryName","value":"Interphotoreceptor retinoid binding protein"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.750.44:FF:000009","properties":[{"key":"EntryName","value":"Retinol-binding protein 3"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.226.10:FF:000037","properties":[{"key":"EntryName","value":"Retinol-binding protein 3"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.90.226.10:FF:000038","properties":[{"key":"EntryName","value":"Retinol-binding protein 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.750.44","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"4"}]},{"database":"Gene3D","id":"3.90.226.10","properties":[{"key":"EntryName","value":"2-enoyl-CoA Hydratase, Chain A, domain 1"},{"key":"MatchStatus","value":"4"}]},{"database":"InterPro","id":"IPR029045","properties":[{"key":"EntryName","value":"ClpP/crotonase-like_dom_sf"}]},{"database":"InterPro","id":"IPR005151","properties":[{"key":"EntryName","value":"Tail-specific_protease"}]},{"database":"PANTHER","id":"PTHR11261","properties":[{"key":"EntryName","value":"INTERPHOTORECEPTOR RETINOID-BINDING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR11261:SF3","properties":[{"key":"EntryName","value":"RETINOL-BINDING PROTEIN 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03572","properties":[{"key":"EntryName","value":"Peptidase_S41"},{"key":"MatchStatus","value":"4"}]},{"database":"Pfam","id":"PF11918","properties":[{"key":"EntryName","value":"Peptidase_S41_N"},{"key":"MatchStatus","value":"4"}]},{"database":"SMART","id":"SM00245","properties":[{"key":"EntryName","value":"TSPc"},{"key":"MatchStatus","value":"4"}]},{"database":"SUPFAM","id":"SSF52096","properties":[{"key":"EntryName","value":"ClpP/crotonase"},{"key":"MatchStatus","value":"4"}]}],"sequence":{"value":"MMREWVLVLSTLLWVPAGPTHLFQPSLVLDMAKILLDNYCFPENLMGMQAAIEQAMKSHEILGISDPQTLAQVLTAGVQSSLSDPRLFISYEPSTLEAPQQAPVLTNLTREELLAQIQRNIRHEVLEGNVGYLRVDDLPGQEVLSELGEFLVSHVWRQLMGTSSLVLDLRHCSGGHFSGIPYVISYLHPGNTVMHVDTVYDRPSNTTTEIWTLPEVLGERYSADKDVVVLTSGHTGGVAEDIAYILKQMRRAIVVGERTEGGALDLQKLRIGQSNFFLTVPVSRSLGPLGGGGQTWEGSGVLPCVGTPAEQALEKALAILTLRRALPGVVLRLQEALQDYYTLVDRVPGLLHHLASMDYSAVVSEEDLVTKLNAGLQAVSEDPRLLVRATGPRDSSSRPETGPNESPAATPEVPTEEDARRALVDSVFQVSVLPGNVGYLRFDRFADAAVLETLGPYVLKQVWEPLQDTEHLIMDLRHNPGGPSSAMPLVLSYFQGPEAGPVRLFTTYDRRTNITQEHFSHRELLGQRYGNQRGVYLLTSHRTATAAEEFAFLMQSLGWATLVGEITAGSLLHTCTVPLLDSPQGGLALTVPVLTFIDNHGEAWLGGGVVPDAIVLAEEALERAQEVLDFHRSLGALVEGTGRLLEAHYARPEIAQRARALLQSKLAQGAYRTAVDLESLASQLTADLQEVSEDHRLLVFHSPGELVAEEVPLPPPAVPSPEELSYLIEALFKTDVLPGQLGYLRFDAMAELETVKAIGPQLVQLVWQRLVDTAALIVDLRYNPGSYSSAVPLLCSYFFEAEPRQHLYSVFDRATSRVTEIWTLPLVAGQRYGSHKDLYILMSHTSGSAAEAFAHTMQDLQRATVIGEPTAGGALSVGIYQVGNSPLYASMPTQMALSASTGEAWDLAGVEPDITVPMSEALSTAQDIVVLRAKVPTVLQTAGKLVADNYASPELGAKMAAKLSGLQSRYARVTSEGALAEMLGADLQILSGDPHLKTAHIPEDAKDRIPGIVPMQIPSPEVFEDLIKFSFHTNVLEDNIGYLRFDMFGDCELLTQVSELLVEHIWKKIVHTDALIIDMRFNLGGPTSSISALCSYFFDEAPPILLDKIYNRPNDSVSELWTHTQLTGERYGSKKSVAILTSGVTAGAAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTHLYITIPTARSVGAEDGSSWEGVGVTPNVVVSSELALTRAKEILQQALRG","length":1234,"molWeight":134451,"crc64":"7884CC3A29D6E7D7","md5":"53E8F155751C9BB3B910BEF50C43382B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Repeat":4,"Region":2,"Glycosylation":3,"Sequence conflict":3},"uniParcId":"UPI00005C9356"}}