{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P47379","secondaryAccessions":["Q49489"],"uniProtkbId":"Y133_MYCGE","entryAudit":{"firstPublicDate":"1996-02-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2017-12-20","entryVersion":118,"sequenceVersion":3},"annotationScore":2.0,"organism":{"scientificName":"Mycoplasmoides genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)","commonName":"Mycoplasma genitalium","taxonId":243273,"lineage":["Bacteria","Bacillati","Mycoplasmatota","Mycoplasmoidales","Mycoplasmoidaceae","Mycoplasmoides"]},"proteinExistence":"4: Predicted","proteinDescription":{"recommendedName":{"fullName":{"value":"Uncharacterized protein MG133"}}},"genes":[{"orderedLocusNames":[{"value":"MG133"}]}],"comments":[{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16407165"}],"value":"Probably essential, it was not disrupted in a global transposon mutagenesis study"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Uncharacterized protein MG133","featureId":"PRO_0000210434"},{"type":"Transmembrane","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":190,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":253,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":237,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"in Ref. 2; CAA43749","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"IPIFASM","alternativeSequences":["SLFLHQC"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"7569993","citationType":"journal article","authors":["Fraser C.M.","Gocayne J.D.","White O.","Adams M.D.","Clayton R.A.","Fleischmann R.D.","Bult C.J.","Kerlavage A.R.","Sutton G.G.","Kelley J.M.","Fritchman J.L.","Weidman J.F.","Small K.V.","Sandusky M.","Fuhrmann J.L.","Nguyen D.T.","Utterback T.R.","Saudek D.M.","Phillips C.A.","Merrick J.M.","Tomb J.-F.","Dougherty B.A.","Bott K.F.","Hu P.-C.","Lucier T.S.","Peterson S.N.","Smith H.O.","Hutchison C.A. III","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"7569993"},{"database":"DOI","id":"10.1126/science.270.5235.397"}],"title":"The minimal gene complement of Mycoplasma genitalium.","publicationDate":"1995","journal":"Science","firstPage":"397","lastPage":"403","volume":"270"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 33530 / DSM 19775 / NCTC 10195 / G37","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"1945886","citationType":"journal article","authors":["Peterson S.N.","Schramm N.","Hu P.-C.","Bott K.F.","Hutchison C.A. III"],"citationCrossReferences":[{"database":"PubMed","id":"1945886"},{"database":"DOI","id":"10.1093/nar/19.21.6027"}],"title":"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium.","publicationDate":"1991","journal":"Nucleic Acids Res.","firstPage":"6027","lastPage":"6031","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 182-244"],"referenceComments":[{"value":"ATCC 33530 / DSM 19775 / NCTC 10195 / G37","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"16407165","citationType":"journal article","authors":["Glass J.I.","Assad-Garcia N.","Alperovich N.","Yooseph S.","Lewis M.R.","Maruf M.","Hutchison C.A. III","Smith H.O.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"16407165"},{"database":"DOI","id":"10.1073/pnas.0510013103"}],"title":"Essential genes of a minimal bacterium.","publicationDate":"2006","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"425","lastPage":"430","volume":"103"},"referencePositions":["SEQUENCE REVISION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"ATCC 33530 / DSM 19775 / NCTC 10195 / G37","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L43967","properties":[{"key":"ProteinId","value":"AAC71350.2"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"X61537","properties":[{"key":"ProteinId","value":"CAA43749.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_009885689.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000908.2"}]},{"database":"AlphaFoldDB","id":"P47379","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"243273.MG_133","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"99646954","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mge:MG_133","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG5032G6H","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_1053017_0_0_14","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P47379","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"399161at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MGEN243273:G1GJ2-147-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000807","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR011631","properties":[{"key":"EntryName","value":"DUF1600"}]},{"database":"Pfam","id":"PF07667","properties":[{"key":"EntryName","value":"DUF1600"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF006834","properties":[{"key":"EntryName","value":"UCP006834"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLKKSIGIFYRCFYLNNKCDYYLIFLAPFSLFTQIFMVITALISVANSGQMSLIWFTNFDTFTYQSNSLAIFLVWYYFLNHKSRWFENSSLVLSVTGYLVFTVIFFNFYALSRFTGIVNIEPDVQGWFSTITTQLPYSFNGSFINDWNAFSELLLHVIHPLFYFIYVGLLFKTYKFIKPPRNLQSFLLKAGIYPSIYAFYLQTIPFLNVWDNGENSYSVYGFFTQTKYNSYVWIWSIPIFASMFLILWMLFVINNHYYGKKHHK","length":264,"molWeight":31419,"crc64":"08035430E82060DC","md5":"0E50C990B56E41BE22A5F862F6A899DC"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Transmembrane":6,"Sequence conflict":1},"uniParcId":"UPI000067DA59"}}