{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P44199","uniProtkbId":"Y1450_HAEIN","entryAudit":{"firstPublicDate":"1995-11-01","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1995-11-01","entryVersion":125,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)","taxonId":71421,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pasteurellales","Pasteurellaceae","Haemophilus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15883182"}],"value":"Putative double-stranded DNA mimic protein HI_1450"}}},"genes":[{"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAC23100.1"}],"value":"HI_1450"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14747986"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15883182"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"15883182"}],"value":"May act as a double-stranded DNA (dsDNA) mimic (PubMed:14747986, PubMed:15883182). Probably regulates the activity of the DNA-binding protein HU (Probable)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14747986"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15883182"}],"value":"Monomer in solution (PubMed:14747986). Interacts with the DNA-binding protein HU (PubMed:15883182)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14747986"}],"value":"Contains many negatively charged residues, which are arranged in winding patterns on the surface similar to the charge distribution seen in dsDNA (PubMed:14747986). The secondary structure elements are arranged in an alpha-alpha-beta-beta-beta-beta order with the two alpha helices packed against the same side of an anti-parallel four-stranded beta sheet (PubMed:14747986)"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00680"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the putative dsDNA mimic protein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"Putative double-stranded DNA mimic protein HI_1450","featureId":"PRO_0000072779"},{"type":"Helix","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Helix","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Beta strand","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Beta strand","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Turn","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Beta strand","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Beta strand","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":92,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Beta strand","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]},{"type":"Beta strand","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1NNV"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"7542800","citationType":"journal article","authors":["Fleischmann R.D.","Adams M.D.","White O.","Clayton R.A.","Kirkness E.F.","Kerlavage A.R.","Bult C.J.","Tomb J.-F.","Dougherty B.A.","Merrick J.M.","McKenney K.","Sutton G.G.","FitzHugh W.","Fields C.A.","Gocayne J.D.","Scott J.D.","Shirley R.","Liu L.-I.","Glodek A.","Kelley J.M.","Weidman J.F.","Phillips C.A.","Spriggs T.","Hedblom E.","Cotton M.D.","Utterback T.R.","Hanna M.C.","Nguyen D.T.","Saudek D.M.","Brandon R.C.","Fine L.D.","Fritchman J.L.","Fuhrmann J.L.","Geoghagen N.S.M.","Gnehm C.L.","McDonald L.A.","Small K.V.","Fraser C.M.","Smith H.O.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"7542800"},{"database":"DOI","id":"10.1126/science.7542800"}],"title":"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.","publicationDate":"1995","journal":"Science","firstPage":"496","lastPage":"512","volume":"269"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 51907 / DSM 11121 / KW20 / Rd","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"15883182","citationType":"journal article","authors":["Parsons L.M.","Liu F.","Orban J."],"citationCrossReferences":[{"database":"PubMed","id":"15883182"},{"database":"DOI","id":"10.1110/ps.041275705"}],"title":"HU-alpha binds to the putative double-stranded DNA mimic HI1450 from Haemophilus influenzae.","publicationDate":"2005","journal":"Protein Sci.","firstPage":"1684","lastPage":"1687","volume":"14"},"referencePositions":["FUNCTION","INTERACTION WITH PROTEIN HU"]},{"referenceNumber":3,"citation":{"id":"14747986","citationType":"journal article","authors":["Parsons L.M.","Yeh D.C.","Orban J."],"citationCrossReferences":[{"database":"PubMed","id":"14747986"},{"database":"DOI","id":"10.1002/prot.10607"}],"title":"Solution structure of the highly acidic protein HI1450 from Haemophilus influenzae, a putative double-stranded DNA mimic.","publicationDate":"2004","journal":"Proteins","firstPage":"375","lastPage":"383","volume":"54"},"referencePositions":["STRUCTURE BY NMR","FUNCTION","SUBUNIT","DOMAIN"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"1NNV"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L42023","properties":[{"key":"ProteinId","value":"AAC23100.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B64030","properties":[{"key":"EntryName","value":"B64030"}]},{"database":"PDB","id":"1NNV","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=1-107"}]},{"database":"PDBsum","id":"1NNV","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P44199","properties":[{"key":"Description","value":"-"}]},{"database":"BMRB","id":"P44199","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P44199","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"71421.HI_1450","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC23100","properties":[{"key":"ProteinId","value":"AAC23100"},{"key":"GeneId","value":"HI_1450"}]},{"database":"KEGG","id":"hin:HI_1450","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|71421.8.peg.1512","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG3099","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_143392_0_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"5677388at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P44199","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"HINF71421:G1GJ1-1476-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"P44199","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000579","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"Gene3D","id":"3.10.450.140","properties":[{"key":"EntryName","value":"dsDNA mimic, putative"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00680","properties":[{"key":"EntryName","value":"Put_dsDNA_mimic"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007376","properties":[{"key":"EntryName","value":"dsDNA_mimic_put"}]},{"database":"InterPro","id":"IPR036763","properties":[{"key":"EntryName","value":"Put_dsDNA_mimic_sf"}]},{"database":"NCBIfam","id":"NF003469","properties":[{"key":"EntryName","value":"PRK05094.1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04269","properties":[{"key":"EntryName","value":"DUF440"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF004916","properties":[{"key":"EntryName","value":"UCP004916"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102816","properties":[{"key":"EntryName","value":"Putative dsDNA mimic"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTTEIKKLDPDTAIDIAYDIFLEMAGENLDPADILLFNLQFEERGGVEFVETADDWEEEIGVLIDPEEYAEVWVGLVNEQDEMDDVFAKFLISHREEDREFHVIWKK","length":107,"molWeight":12525,"crc64":"B585C2B9E3E29A8B","md5":"8FD3BDE4F7D86CC6AEBF5F8AB1C96AB8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"DOMAIN":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Helix":2,"Beta strand":6,"Turn":1},"uniParcId":"UPI000013A6CF"}}