{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P42949","secondaryAccessions":["D6VW80","Q6B2X3"],"uniProtkbId":"TIM16_YEAST","entryAudit":{"firstPublicDate":"1995-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1995-11-01","entryVersion":182,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Mitochondrial import inner membrane translocase subunit TIM16"}},"alternativeNames":[{"fullName":{"value":"Presequence translocated-associated motor subunit PAM16"}}]},"genes":[{"geneName":{"value":"PAM16"},"synonyms":[{"value":"TIM16"}],"orderedLocusNames":[{"value":"YJL104W"}],"orfNames":[{"value":"J0822"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981506"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981507"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15218029"}],"value":"Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981506"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981507"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15218029"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16027163"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16105940"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16107694"}],"value":"Homodimer and heterodimer with PAM18. Homodimerization may not be relevant in vivo, while heterodimerization is essential for activity regulation of mtHSP70. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18. Interacts with MDJ2"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P42949","intActId":"EBI-26019"},"interactantTwo":{"uniProtKBAccession":"Q07914","geneName":"PAM18","intActId":"EBI-31963"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P42949","intActId":"EBI-26019"},"interactantTwo":{"uniProtKBAccession":"P39515","geneName":"TIM17","intActId":"EBI-9127"},"numberOfExperiments":4,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P42949","intActId":"EBI-26019"},"interactantTwo":{"uniProtKBAccession":"P32897","geneName":"TIM23","intActId":"EBI-9136"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P42949","intActId":"EBI-26019"},"interactantTwo":{"uniProtKBAccession":"Q01852","geneName":"TIM44","intActId":"EBI-9141"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981506"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981507"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981506"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14981507"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16105940"}],"value":"The J-like region, although related to the J domain does not stimulate ATPase activity of mtHSP70. It nevertheless mediates the heterodimerization with the J domain of PAM18 and is therefore essential for PAM complex function"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 3180 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TIM16/PAM16 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"Mitochondrial import inner membrane translocase subunit TIM16","featureId":"PRO_0000214098"},{"type":"Region","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"J-like"},{"type":"Region","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":149,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Mutagenesis","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"Does not confer ability to stimulate the ATPase activity of mtHSP70.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15218029"}],"alternativeSequence":{"originalSequence":"DKE","alternativeSequences":["HPD"]}},{"type":"Sequence conflict","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"in Ref. 4; AAT92626","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["H"]}},{"type":"Helix","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2GUZ"}]},{"type":"Helix","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2GUZ"}]},{"type":"Helix","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2GUZ"}]},{"type":"Helix","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2GUZ"}]},{"type":"Helix","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2GUZ"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0811","category":"Biological process","name":"Translocation"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"7483851","citationType":"journal article","authors":["Rasmussen S.W."],"citationCrossReferences":[{"database":"PubMed","id":"7483851"},{"database":"DOI","id":"10.1002/yea.320110909"}],"title":"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg).","publicationDate":"1995","journal":"Yeast","firstPage":"873","lastPage":"883","volume":"11"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 96604 / S288c / FY1679","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"8641269","citationType":"journal article","authors":["Galibert F.","Alexandraki D.","Baur A.","Boles E.","Chalwatzis N.","Chuat J.-C.","Coster F.","Cziepluch C.","de Haan M.","Domdey H.","Durand P.","Entian K.-D.","Gatius M.","Goffeau A.","Grivell L.A.","Hennemann A.","Herbert C.J.","Heumann K.","Hilger F.","Hollenberg C.P.","Huang M.-E.","Jacq C.","Jauniaux J.-C.","Katsoulou C.","Kirchrath L.","Kleine K.","Kordes E.","Koetter P.","Liebl S.","Louis E.J.","Manus V.","Mewes H.-W.","Miosga T.","Obermaier B.","Perea J.","Pohl T.M.","Portetelle D.","Pujol A.","Purnelle B.","Ramezani Rad M.","Rasmussen S.W.","Rose M.","Rossau R.","Schaaff-Gerstenschlaeger I.","Smits P.H.M.","Scarcez T.","Soriano N.","To Van D.","Tzermia M.","Van Broekhoven A.","Vandenbol M.","Wedler H.","von Wettstein D.","Wambutt R.","Zagulski M.","Zollner A.","Karpfinger-Hartl L."],"citationCrossReferences":[{"database":"PubMed","id":"8641269"},{"database":"DOI","id":"10.1002/j.1460-2075.1996.tb00557.x"}],"title":"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.","publicationDate":"1996","journal":"EMBO J.","firstPage":"2031","lastPage":"2049","volume":"15"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"17322287","citationType":"journal article","authors":["Hu Y.","Rolfs A.","Bhullar B.","Murthy T.V.S.","Zhu C.","Berger M.F.","Camargo A.A.","Kelley F.","McCarron S.","Jepson D.","Richardson A.","Raphael J.","Moreira D.","Taycher E.","Zuo D.","Mohr S.","Kane M.F.","Williamson J.","Simpson A.J.G.","Bulyk M.L.","Harlow E.","Marsischky G.","Kolodner R.D.","LaBaer J."],"citationCrossReferences":[{"database":"PubMed","id":"17322287"},{"database":"DOI","id":"10.1101/gr.6037607"}],"title":"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.","publicationDate":"2007","journal":"Genome Res.","firstPage":"536","lastPage":"543","volume":"17"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"15218029","citationType":"journal article","authors":["Li Y.","Dudek J.","Guiard B.","Pfanner N.","Rehling P.","Voos W."],"citationCrossReferences":[{"database":"PubMed","id":"15218029"},{"database":"DOI","id":"10.1074/jbc.m404319200"}],"title":"The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18.","publicationDate":"2004","journal":"J. Biol. Chem.","firstPage":"38047","lastPage":"38054","volume":"279"},"referencePositions":["FUNCTION","INTERACTION WITH PAM18","MUTAGENESIS OF 88-ASP--GLU-90"]},{"referenceNumber":7,"citation":{"id":"14981507","citationType":"journal article","authors":["Frazier A.E.","Dudek J.","Guiard B.","Voos W.","Li Y.","Lind M.","Meisinger C.","Geissler A.","Sickmann A.","Meyer H.E.","Bilanchone V.","Cumsky M.G.","Truscott K.N.","Pfanner N.","Rehling P."],"citationCrossReferences":[{"database":"PubMed","id":"14981507"},{"database":"DOI","id":"10.1038/nsmb735"}],"title":"Pam16 has an essential role in the mitochondrial protein import motor.","publicationDate":"2004","journal":"Nat. Struct. Mol. Biol.","firstPage":"226","lastPage":"233","volume":"11"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY","IDENTIFICATION IN THE PAM COMPLEX","FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH PAM18"]},{"referenceNumber":8,"citation":{"id":"14981506","citationType":"journal article","authors":["Kozany C.","Mokranjac D.","Sichting M.","Neupert W.","Hell K."],"citationCrossReferences":[{"database":"PubMed","id":"14981506"},{"database":"DOI","id":"10.1038/nsmb734"}],"title":"The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase.","publicationDate":"2004","journal":"Nat. Struct. Mol. Biol.","firstPage":"234","lastPage":"241","volume":"11"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY","IDENTIFICATION IN THE PAM COMPLEX","FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":9,"citation":{"id":"16027163","citationType":"journal article","authors":["Mokranjac D.","Sichting M.","Popov-Celeketic D.","Berg A.","Hell K.","Neupert W."],"citationCrossReferences":[{"database":"PubMed","id":"16027163"},{"database":"DOI","id":"10.1074/jbc.m502397200"}],"title":"The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2.","publicationDate":"2005","journal":"J. Biol. Chem.","firstPage":"31608","lastPage":"31614","volume":"280"},"referencePositions":["INTERACTION WITH MDJ2"]},{"referenceNumber":10,"citation":{"id":"16107694","citationType":"journal article","authors":["van der Laan M.","Chacinska A.","Lind M.","Perschil I.","Sickmann A.","Meyer H.E.","Guiard B.","Meisinger C.","Pfanner N.","Rehling P."],"citationCrossReferences":[{"database":"PubMed","id":"16107694"},{"database":"DOI","id":"10.1128/mcb.25.17.7449-7458.2005"}],"title":"Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor.","publicationDate":"2005","journal":"Mol. Cell. Biol.","firstPage":"7449","lastPage":"7458","volume":"25"},"referencePositions":["IDENTIFICATION IN THE PAM COMPLEX WITH PAM17; PAM18; TIM44; SSC1 AND MGE1"]},{"referenceNumber":11,"citation":{"id":"16105940","citationType":"journal article","authors":["D'Silva P.R.","Schilke B.","Walter W.","Craig E.A."],"citationCrossReferences":[{"database":"PubMed","id":"16105940"},{"database":"DOI","id":"10.1073/pnas.0505969102"}],"title":"Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane.","publicationDate":"2005","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"12419","lastPage":"12424","volume":"102"},"referencePositions":["SUBUNIT","DOMAIN J","INTERACTION WITH PAM18"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X85021","properties":[{"key":"ProteinId","value":"CAA59390.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z49379","properties":[{"key":"ProteinId","value":"CAA89399.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY692607","properties":[{"key":"ProteinId","value":"AAT92626.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006943","properties":[{"key":"ProteinId","value":"DAA08696.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S53383","properties":[{"key":"EntryName","value":"S53383"}]},{"database":"RefSeq","id":"NP_012431.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001181537.1"}]},{"database":"PDB","id":"2GUZ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.00 A"},{"key":"Chains","value":"B/D/F/H/J/L/N/P=54-117"}]},{"database":"PDBsum","id":"2GUZ","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"33652","properties":[{"key":"Interactions","value":"459"}]},{"database":"ComplexPortal","id":"CPX-539","properties":[{"key":"EntryName","value":"TIM23 mitochondrial inner membrane pre-sequence translocase complex, motor variant"}]},{"database":"FunCoup","id":"P42949","properties":[{"key":"Number of interactors","value":"323"}]},{"database":"IntAct","id":"P42949","properties":[{"key":"Interactions","value":"10"}]},{"database":"MINT","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YJL104W","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.A.8.1.1","properties":[{"key":"FamilyName","value":"the mitochondrial protein translocase (mpt) family"}]},{"database":"iPTMnet","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YJL104W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YJL104W_mRNA","properties":[{"key":"ProteinId","value":"YJL104W"},{"key":"GeneId","value":"YJL104W"}]},{"database":"GeneID","id":"853340","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YJL104W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000003640","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000003640","properties":[{"key":"GeneName","value":"PAM16"}]},{"database":"VEuPathDB","id":"FungiDB:YJL104W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3442","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_101461_0_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"RMFKIND","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10262892at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-31558-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"853340","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"P42949","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P42949","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome X"}]},{"database":"RNAct","id":"P42949","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"TAS:Reactome"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14576278"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"16823961"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"24769239"}]},{"database":"GO","id":"GO:0001405","properties":[{"key":"GoTerm","value":"C:PAM complex, Tim23 associated import motor"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14981506"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14981507"}]},{"database":"GO","id":"GO:0005744","properties":[{"key":"GoTerm","value":"C:TIM23 mitochondrial import inner membrane translocase complex"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"14981506"}]},{"database":"GO","id":"GO:0019904","properties":[{"key":"GoTerm","value":"F:protein domain specific binding"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16105940"}]},{"database":"GO","id":"GO:0006886","properties":[{"key":"GoTerm","value":"P:intracellular protein transport"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16107694"}]},{"database":"GO","id":"GO:0030150","properties":[{"key":"GoTerm","value":"P:protein import into mitochondrial matrix"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"14981506"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"14981507"}]},{"database":"FunFam","id":"1.10.287.110:FF:000006","properties":[{"key":"EntryName","value":"Import inner membrane translocase subunit TIM16"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.287.110","properties":[{"key":"EntryName","value":"DnaJ domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR036869","properties":[{"key":"EntryName","value":"J_dom_sf"}]},{"database":"InterPro","id":"IPR005341","properties":[{"key":"EntryName","value":"Tim16"}]},{"database":"PANTHER","id":"PTHR12388","properties":[{"key":"EntryName","value":"MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12388:SF0","properties":[{"key":"EntryName","value":"MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03656","properties":[{"key":"EntryName","value":"Pam16"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAHRAFIQVIITGTQVFGKAFAEAYRQAASQSVKQGATNASRRGTGKGEYGGITLDESCKILNIEESKGDLNMDKINNRFNYLFEVNDKEKGGSFYLQSKVYRAAERLKWELAQREKNAKAKAGDASTAKPPPNSTNSSGADNSASSNQ","length":149,"molWeight":16216,"crc64":"42BB69D746264B47","md5":"7552E0BABFBB0C45A5401D0F58F111FD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":4,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":2,"Compositional bias":1,"Mutagenesis":1,"Sequence conflict":1,"Helix":5},"uniParcId":"UPI000013B5E0"}}