{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P40080","secondaryAccessions":["D3DM34"],"uniProtkbId":"VFA1_YEAST","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1995-02-01","entryVersion":136,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"VPS4-associated protein 1"}}},"genes":[{"geneName":{"value":"VFA1"},"orderedLocusNames":[{"value":"YER128W"}],"orfNames":[{"value":"SYGP-ORF44"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21777356"}],"value":"VPS4-associated protein involved in trafficking to the vacuole"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21777356"}],"value":"Interacts with VPS4"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Requires VPS4 for its recruitment to endosomes"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21777356"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21777356"}],"value":"Endosome","id":"SL-0101"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 967 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":203,"modifier":"EXACT"}},"description":"VPS4-associated protein 1","featureId":"PRO_0000202649"},{"type":"Region","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":171,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Helix","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5FVK"}]},{"type":"Helix","location":{"start":{"value":198,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5FVK"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9169868","citationType":"journal article","authors":["Dietrich F.S.","Mulligan J.T.","Hennessy K.M.","Yelton M.A.","Allen E.","Araujo R.","Aviles E.","Berno A.","Brennan T.","Carpenter J.","Chen E.","Cherry J.M.","Chung E.","Duncan M.","Guzman E.","Hartzell G.","Hunicke-Smith S.","Hyman R.W.","Kayser A.","Komp C.","Lashkari D.","Lew H.","Lin D.","Mosedale D.","Nakahara K.","Namath A.","Norgren R.","Oefner P.","Oh C.","Petel F.X.","Roberts D.","Sehl P.","Schramm S.","Shogren T.","Smith V.","Taylor P.","Wei Y.","Botstein D.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"9169868"},{"database":"DOI","id":"10.1038/387s078"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome V.","publicationDate":"1997","journal":"Nature","firstPage":"78","lastPage":"81","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":4,"citation":{"id":"21777356","citationType":"journal article","authors":["Arlt H.","Perz A.","Ungermann C."],"citationCrossReferences":[{"database":"PubMed","id":"21777356"},{"database":"DOI","id":"10.1111/j.1600-0854.2011.01252.x"}],"title":"An overexpression screen in Saccharomyces cerevisiae identifies novel genes that affect endocytic protein trafficking.","publicationDate":"2011","journal":"Traffic","firstPage":"1592","lastPage":"1603","volume":"12"},"referencePositions":["INTERACTION WITH VPS4","SUBCELLULAR LOCATION","FUNCTION"]},{"referenceNumber":5,"citation":{"id":"22814378","citationType":"journal article","authors":["Van Damme P.","Lasa M.","Polevoda B.","Gazquez C.","Elosegui-Artola A.","Kim D.S.","De Juan-Pardo E.","Demeyer K.","Hole K.","Larrea E.","Timmerman E.","Prieto J.","Arnesen T.","Sherman F.","Gevaert K.","Aldabe R."],"citationCrossReferences":[{"database":"PubMed","id":"22814378"},{"database":"DOI","id":"10.1073/pnas.1210303109"}],"title":"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.","publicationDate":"2012","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"12449","lastPage":"12454","volume":"109"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U18916","properties":[{"key":"ProteinId","value":"AAC03226.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006939","properties":[{"key":"ProteinId","value":"DAA07788.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S43222","properties":[{"key":"EntryName","value":"S43222"}]},{"database":"RefSeq","id":"NP_011054.3","properties":[{"key":"NucleotideSequenceId","value":"NM_001179018.3"}]},{"database":"PDB","id":"4NIQ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"C/D=182-203"}]},{"database":"PDB","id":"5FVK","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.66 A"},{"key":"Chains","value":"C/D=182-203"}]},{"database":"PDBsum","id":"4NIQ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5FVK","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P40080","properties":[{"key":"Description","value":"-"}]},{"database":"MDposit","id":"P40080","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P40080","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"36872","properties":[{"key":"Interactions","value":"95"}]},{"database":"DIP","id":"DIP-5406N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P40080","properties":[{"key":"Number of interactors","value":"34"}]},{"database":"IntAct","id":"P40080","properties":[{"key":"Interactions","value":"3"}]},{"database":"STRING","id":"4932.YER128W","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"P40080","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YER128W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P40080","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"856865","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YER128W_mRNA","properties":[{"key":"ProteinId","value":"YER128W"},{"key":"GeneId","value":"YER128W"}]},{"database":"GeneID","id":"856865","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YER128W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000000930","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000000930","properties":[{"key":"GeneName","value":"VFA1"}]},{"database":"VEuPathDB","id":"FungiDB:YER128W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502RW3H","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_088285_2_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P40080","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FYVCPAH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2158714at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-30291-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"856865","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:P40080","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome V"}]},{"database":"RNAct","id":"P40080","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21777356"}]},{"database":"GO","id":"GO:0005768","properties":[{"key":"GoTerm","value":"C:endosome"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21777356"}]},{"database":"GO","id":"GO:0001671","properties":[{"key":"GoTerm","value":"F:ATPase activator activity"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"24567329"}]},{"database":"GO","id":"GO:0007034","properties":[{"key":"GoTerm","value":"P:vacuolar transport"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"21777356"}]},{"database":"InterPro","id":"IPR013640","properties":[{"key":"EntryName","value":"Vfa1"}]},{"database":"PANTHER","id":"PTHR28218","properties":[{"key":"EntryName","value":"VPS4-ASSOCIATED PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR28218:SF1","properties":[{"key":"EntryName","value":"VPS4-ASSOCIATED PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08432","properties":[{"key":"EntryName","value":"Vfa1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MINEYVARKVALKDMQPCAICSKPSTTVLYNASGPDWLYTCEIHLQDNPQFVIPLYSTEYNEAVAQLKLVKGKMDSLTSAQTQLGSWDGWVTKIFSKKEKETNNSKDPDPTTTDSTDTSPQAKNDAEILSETKKQYSKILDKVTELQRKNRKYELAKIMFESRLLRKRTEQVNRERYLKEQENYSNTDPEELLRKHVFPSVPK","length":203,"molWeight":23489,"crc64":"819930C50DD56B1D","md5":"84A0265BB3595BCA310E087E028640EE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1},"countByFeatureType":{"Chain":1,"Region":2,"Coiled coil":1,"Compositional bias":3,"Helix":2},"uniParcId":"UPI000013ACC2"}}