{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P39829","secondaryAccessions":["Q2M981"],"uniProtkbId":"GARD_ECOLI","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1995-11-01","entryVersion":157,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9772162"}],"value":"Galactarate dehydratase (L-threo-forming)"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9772162"}],"value":"GalcD"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}],"value":"4.2.1.42"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"10762278"}],"value":"garD"},"synonyms":[{"value":"yhaG"}],"orderedLocusNames":[{"value":"b3128"},{"value":"JW3097"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31811683"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}],"value":"Catalyzes the dehydration of galactarate to form 5-dehydro-4-deoxy-D-glucarate (5-KDG)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"galactarate = 5-dehydro-4-deoxy-D-glucarate + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:16005"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:16537"},{"database":"ChEBI","id":"CHEBI:42819"}],"ecNumber":"4.2.1.42","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31811683"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:16006"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31811683"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31811683"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29033"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":800.0,"unit":"uM","substrate":"galactarate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}],"value":"kcat is 22 sec(-1)."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9772162"}],"value":"Carbohydrate acid metabolism; galactarate degradation; D-glycerate from galactarate: step 1/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31811683"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10762278"}],"value":"Induced by galactarate, D-glucarate and D-glycerate"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31811683"}],"value":"Consists of three domains. The N-terminal SAF domain, which forms a beta-clip fold, is likely involved in the substrate recognition. It is connected by a long unstructured linker to the second domain, which serves as a dimerization interface between two monomers. The C-terminal domain represents the catalytic core of the protein and probably contains the metal binding site"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_02031"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UxaA family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":523,"modifier":"EXACT"}},"description":"Galactarate dehydratase (L-threo-forming)","featureId":"PRO_0000172285"},{"type":"Helix","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Helix","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Beta strand","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Beta strand","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Beta strand","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Beta strand","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Helix","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3LAZ"}]},{"type":"Helix","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":101,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":135,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":159,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":169,"modifier":"EXACT"},"end":{"value":172,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":189,"modifier":"EXACT"},"end":{"value":198,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":275,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":279,"modifier":"EXACT"},"end":{"value":281,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":295,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":299,"modifier":"EXACT"},"end":{"value":312,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":316,"modifier":"EXACT"},"end":{"value":319,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":322,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":326,"modifier":"EXACT"},"end":{"value":331,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":336,"modifier":"EXACT"},"end":{"value":338,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":339,"modifier":"EXACT"},"end":{"value":355,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":386,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":387,"modifier":"EXACT"},"end":{"value":390,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":395,"modifier":"EXACT"},"end":{"value":399,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":409,"modifier":"EXACT"},"end":{"value":413,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":419,"modifier":"EXACT"},"end":{"value":429,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":432,"modifier":"EXACT"},"end":{"value":440,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":447,"modifier":"EXACT"},"end":{"value":449,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":451,"modifier":"EXACT"},"end":{"value":457,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Turn","location":{"start":{"value":464,"modifier":"EXACT"},"end":{"value":467,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Beta strand","location":{"start":{"value":469,"modifier":"EXACT"},"end":{"value":471,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Turn","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":478,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":482,"modifier":"EXACT"},"end":{"value":497,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":508,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]},{"type":"Helix","location":{"start":{"value":512,"modifier":"EXACT"},"end":{"value":514,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"6U7L"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. III","Bloch C.A.","Perna N.T.","Burland V.","Riley M.","Collado-Vides J.","Glasner J.D.","Rode C.K.","Mayhew G.F.","Gregor J.","Davis N.W.","Kirkpatrick H.A.","Goeden M.A.","Rose D.J.","Mau B.","Shao Y."],"citationCrossReferences":[{"database":"PubMed","id":"9278503"},{"database":"DOI","id":"10.1126/science.277.5331.1453"}],"title":"The complete genome sequence of Escherichia coli K-12.","publicationDate":"1997","journal":"Science","firstPage":"1453","lastPage":"1462","volume":"277"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / MG1655 / ATCC 47076","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16738553","citationType":"journal article","authors":["Hayashi K.","Morooka N.","Yamamoto Y.","Fujita K.","Isono K.","Choi S.","Ohtsubo E.","Baba T.","Wanner B.L.","Mori H.","Horiuchi T."],"citationCrossReferences":[{"database":"PubMed","id":"16738553"},{"database":"DOI","id":"10.1038/msb4100049"}],"title":"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.","publicationDate":"2006","journal":"Mol. Syst. Biol.","firstPage":"E1","lastPage":"E5","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"K12 / W3110 / ATCC 27325 / DSM 5911","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"2407727","citationType":"journal article","authors":["Baird L.","Georgopoulos C."],"citationCrossReferences":[{"database":"PubMed","id":"2407727"},{"database":"DOI","id":"10.1128/jb.172.3.1587-1594.1990"}],"title":"Identification, cloning, and characterization of the Escherichia coli sohA gene, a suppressor of the htrA (degP) null phenotype.","publicationDate":"1990","journal":"J. Bacteriol.","firstPage":"1587","lastPage":"1594","volume":"172"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 449-523"]},{"referenceNumber":4,"citation":{"id":"CI-DTI9JMGGTG2DS","citationType":"unpublished observations","authors":["Rudd K.E."],"publicationDate":"DEC-1994"},"referencePositions":["IDENTIFICATION"]},{"referenceNumber":5,"citation":{"id":"9772162","citationType":"journal article","authors":["Hubbard B.K.","Koch M.","Palmer D.R.","Babbitt P.C.","Gerlt J.A."],"citationCrossReferences":[{"database":"PubMed","id":"9772162"},{"database":"DOI","id":"10.1021/bi981124f"}],"title":"Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli.","publicationDate":"1998","journal":"Biochemistry","firstPage":"14369","lastPage":"14375","volume":"37"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","PATHWAY"]},{"referenceNumber":6,"citation":{"id":"10762278","citationType":"journal article","authors":["Monterrubio R.","Baldoma L.","Obradors N.","Aguilar J.","Badia J."],"citationCrossReferences":[{"database":"PubMed","id":"10762278"},{"database":"DOI","id":"10.1128/jb.182.9.2672-2674.2000"}],"title":"A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli.","publicationDate":"2000","journal":"J. Bacteriol.","firstPage":"2672","lastPage":"2674","volume":"182"},"referencePositions":["GENE NAME","INDUCTION"]},{"referenceNumber":7,"citation":{"id":"CI-EUM3F1LU8990P","citationType":"submission","authoringGroup":["Midwest center for structural genomics (MCSG)"],"title":"The crystal structure of the N-terminal domain of D-galactarate dehydratase from Escherichia coli CFT073.","publicationDate":"JAN-2010","submissionDatabase":"PDB data bank"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 6-96"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"3LAZ"}]},{"referenceNumber":8,"citation":{"id":"31811683","citationType":"journal article","authors":["Rosas-Lemus M.","Minasov G.","Shuvalova L.","Wawrzak Z.","Kiryukhina O.","Mih N.","Jaroszewski L.","Palsson B.","Godzik A.","Satchell K.J.F."],"citationCrossReferences":[{"database":"PubMed","id":"31811683"},{"database":"DOI","id":"10.1002/pro.3796"}],"title":"Structure of galactarate dehydratase, a new fold in an enolase involved in bacterial fitness after antibiotic treatment.","publicationDate":"2020","journal":"Protein Sci.","firstPage":"711","lastPage":"722","volume":"29"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) IN COMPLEX WITH CALCIUM","FUNCTION","CATALYTIC ACTIVITY","COFACTOR","SUBUNIT","DOMAIN"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"6U7L"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U18997","properties":[{"key":"ProteinId","value":"AAA57931.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U00096","properties":[{"key":"ProteinId","value":"AAC76162.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AP009048","properties":[{"key":"ProteinId","value":"BAE77175.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"M30178","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"D65102","properties":[{"key":"EntryName","value":"D65102"}]},{"database":"RefSeq","id":"NP_417597.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000913.3"}]},{"database":"RefSeq","id":"WP_001273753.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_CP009789.1"}]},{"database":"PDB","id":"3LAZ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.92 A"},{"key":"Chains","value":"A/B=1-96"}]},{"database":"PDB","id":"6U7L","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.75 A"},{"key":"Chains","value":"A/B/C/D=1-523"}]},{"database":"PDBsum","id":"3LAZ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"6U7L","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"4259485","properties":[{"key":"Interactions","value":"29"}]},{"database":"BioGRID","id":"851954","properties":[{"key":"Interactions","value":"1"}]},{"database":"FunCoup","id":"P39829","properties":[{"key":"Number of interactors","value":"333"}]},{"database":"IntAct","id":"P39829","properties":[{"key":"Interactions","value":"5"}]},{"database":"STRING","id":"511145.b3128","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"511145-b3128","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAC76162","properties":[{"key":"ProteinId","value":"AAC76162"},{"key":"GeneId","value":"b3128"}]},{"database":"GeneID","id":"947641","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecj:JW3097","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"eco:b3128","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ecoc:C3026_17050","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|1411691.4.peg.3603","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"EchoBASE","id":"EB2413","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG2721","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_029189_0_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"VGWELFH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9804574at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"EcoCyc:GALACTARDEHYDRA-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:GALACTARDEHYDRA-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.1.42","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"SABIO-RK","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00565","properties":[{"key":"RectionId","value":"UER00629"}]},{"database":"EvolutionaryTrace","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P39829","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000625","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P39829","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0008198","properties":[{"key":"GoTerm","value":"F:ferrous iron binding"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9772162"}]},{"database":"GO","id":"GO:0008867","properties":[{"key":"GoTerm","value":"F:galactarate dehydratase activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9772162"}]},{"database":"GO","id":"GO:0042803","properties":[{"key":"GoTerm","value":"F:protein homodimerization activity"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31811683"}]},{"database":"GO","id":"GO:0046392","properties":[{"key":"GoTerm","value":"P:D-galactarate catabolic process"},{"key":"GoEvidenceType","value":"IDA:EcoCyc"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"9772162"}]},{"database":"GO","id":"GO:0019698","properties":[{"key":"GoTerm","value":"P:D-galacturonate catabolic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd11613","properties":[{"key":"EntryName","value":"SAF_AH_GD"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.130.110:FF:000001","properties":[{"key":"EntryName","value":"Galactarate dehydratase (L-threo-forming)"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.130.110","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_02031","properties":[{"key":"EntryName","value":"Galactar_dehydrat"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR017654","properties":[{"key":"EntryName","value":"GarD-like"}]},{"database":"InterPro","id":"IPR032893","properties":[{"key":"EntryName","value":"GarD_Enterobacteriaceae"}]},{"database":"InterPro","id":"IPR048332","properties":[{"key":"EntryName","value":"GD_AH_C"}]},{"database":"InterPro","id":"IPR007392","properties":[{"key":"EntryName","value":"GD_AH_second"}]},{"database":"InterPro","id":"IPR013974","properties":[{"key":"EntryName","value":"SAF"}]},{"database":"InterPro","id":"IPR044144","properties":[{"key":"EntryName","value":"SAF_UxaA/GarD"}]},{"database":"InterPro","id":"IPR052172","properties":[{"key":"EntryName","value":"UxaA_altronate/galactarate_dh"}]},{"database":"NCBIfam","id":"TIGR03248","properties":[{"key":"EntryName","value":"galactar-dH20"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30536","properties":[{"key":"EntryName","value":"ALTRONATE/GALACTARATE DEHYDRATASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30536:SF1","properties":[{"key":"EntryName","value":"GALACTARATE DEHYDRATASE (L-THREO-FORMING)"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF20629","properties":[{"key":"EntryName","value":"GD_AH_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04295","properties":[{"key":"EntryName","value":"GD_AH_second"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF30644","properties":[{"key":"EntryName","value":"SAF_GD_AH"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00858","properties":[{"key":"EntryName","value":"SAF"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLELIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTLPLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDLLPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKLQPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRETCPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTPRAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGKSAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVPVIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQWGLHNQLAVFNPAPVT","length":523,"molWeight":56402,"crc64":"919BCC8B49411BB0","md5":"78261D16FF6656AC479F4550B2DDD7E2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"INDUCTION":1,"DOMAIN":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Helix":25,"Beta strand":20,"Turn":2},"uniParcId":"UPI000012B0DE"}}