{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P39695","uniProtkbId":"COMEC_BACSU","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-08-04","entryVersion":148,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Bacillus subtilis (strain 168)","taxonId":224308,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"ComE operon protein 3"}}},"genes":[{"geneName":{"value":"comEC"},"synonyms":[{"value":"comE3"}],"orderedLocusNames":[{"value":"BSU25570"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"7968523"}],"value":"The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11948146"}],"value":"Expression activated by ComK (PubMed:11948146)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17630974"}],"value":"Destabilization of ComFA (PubMed:17630974)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":776,"modifier":"EXACT"}},"description":"ComE operon protein 3","featureId":"PRO_0000090009"},{"type":"Transmembrane","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":232,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":308,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":352,"modifier":"EXACT"},"end":{"value":372,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":387,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":416,"modifier":"EXACT"},"end":{"value":436,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":443,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":475,"modifier":"EXACT"},"end":{"value":495,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":451,"modifier":"EXACT"},"end":{"value":451,"modifier":"EXACT"}},"description":"in Ref. 1; AAC36907","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["I"]}},{"type":"Sequence conflict","location":{"start":{"value":639,"modifier":"EXACT"},"end":{"value":639,"modifier":"EXACT"}},"description":"in Ref. 1; AAC36907","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["K"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0178","category":"Biological process","name":"Competence"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"7968523","citationType":"journal article","authors":["Hahn J.","Inamine G.","Kozlov Y.","Dubnau D.A."],"citationCrossReferences":[{"database":"PubMed","id":"7968523"},{"database":"DOI","id":"10.1111/j.1365-2958.1993.tb00907.x"}],"title":"Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNA.","publicationDate":"1993","journal":"Mol. Microbiol.","firstPage":"99","lastPage":"110","volume":"10"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION"]},{"referenceNumber":2,"citation":{"id":"8969508","citationType":"journal article","authors":["Mizuno M.","Masuda S.","Takemaru K.","Hosono S.","Sato T.","Takeuchi M.","Kobayashi Y."],"citationCrossReferences":[{"database":"PubMed","id":"8969508"},{"database":"DOI","id":"10.1099/13500872-142-11-3103"}],"title":"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.","publicationDate":"1996","journal":"Microbiology","firstPage":"3103","lastPage":"3111","volume":"142"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"168 / JH642","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"9384377","citationType":"journal article","authors":["Kunst F.","Ogasawara N.","Moszer I.","Albertini A.M.","Alloni G.","Azevedo V.","Bertero M.G.","Bessieres P.","Bolotin A.","Borchert S.","Borriss R.","Boursier L.","Brans A.","Braun M.","Brignell S.C.","Bron S.","Brouillet S.","Bruschi C.V.","Caldwell B.","Capuano V.","Carter N.M.","Choi S.-K.","Codani J.-J.","Connerton I.F.","Cummings N.J.","Daniel R.A.","Denizot F.","Devine K.M.","Duesterhoeft A.","Ehrlich S.D.","Emmerson P.T.","Entian K.-D.","Errington J.","Fabret C.","Ferrari E.","Foulger D.","Fritz C.","Fujita M.","Fujita Y.","Fuma S.","Galizzi A.","Galleron N.","Ghim S.-Y.","Glaser P.","Goffeau A.","Golightly E.J.","Grandi G.","Guiseppi G.","Guy B.J.","Haga K.","Haiech J.","Harwood C.R.","Henaut A.","Hilbert H.","Holsappel S.","Hosono S.","Hullo M.-F.","Itaya M.","Jones L.-M.","Joris B.","Karamata D.","Kasahara Y.","Klaerr-Blanchard M.","Klein C.","Kobayashi Y.","Koetter P.","Koningstein G.","Krogh S.","Kumano M.","Kurita K.","Lapidus A.","Lardinois S.","Lauber J.","Lazarevic V.","Lee S.-M.","Levine A.","Liu H.","Masuda S.","Mauel C.","Medigue C.","Medina N.","Mellado R.P.","Mizuno M.","Moestl D.","Nakai S.","Noback M.","Noone D.","O'Reilly M.","Ogawa K.","Ogiwara A.","Oudega B.","Park S.-H.","Parro V.","Pohl T.M.","Portetelle D.","Porwollik S.","Prescott A.M.","Presecan E.","Pujic P.","Purnelle B.","Rapoport G.","Rey M.","Reynolds S.","Rieger M.","Rivolta C.","Rocha E.","Roche B.","Rose M.","Sadaie Y.","Sato T.","Scanlan E.","Schleich S.","Schroeter R.","Scoffone F.","Sekiguchi J.","Sekowska A.","Seror S.J.","Serror P.","Shin B.-S.","Soldo B.","Sorokin A.","Tacconi E.","Takagi T.","Takahashi H.","Takemaru K.","Takeuchi M.","Tamakoshi A.","Tanaka T.","Terpstra P.","Tognoni A.","Tosato V.","Uchiyama S.","Vandenbol M.","Vannier F.","Vassarotti A.","Viari A.","Wambutt R.","Wedler E.","Wedler H.","Weitzenegger T.","Winters P.","Wipat A.","Yamamoto H.","Yamane K.","Yasumoto K.","Yata K.","Yoshida K.","Yoshikawa H.-F.","Zumstein E.","Yoshikawa H.","Danchin A."],"citationCrossReferences":[{"database":"PubMed","id":"9384377"},{"database":"DOI","id":"10.1038/36786"}],"title":"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.","publicationDate":"1997","journal":"Nature","firstPage":"249","lastPage":"256","volume":"390"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"168","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"11948146","citationType":"journal article","authors":["Ogura M.","Yamaguchi H.","Kobayashi K.","Ogasawara N.","Fujita Y.","Tanaka T."],"citationCrossReferences":[{"database":"PubMed","id":"11948146"},{"database":"DOI","id":"10.1128/jb.184.9.2344-2351.2002"}],"title":"Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.","publicationDate":"2002","journal":"J. Bacteriol.","firstPage":"2344","lastPage":"2351","volume":"184"},"referencePositions":["INDUCTION"],"referenceComments":[{"value":"168 / CU741","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"17630974","citationType":"journal article","authors":["Kramer N.","Hahn J.","Dubnau D."],"citationCrossReferences":[{"database":"PubMed","id":"17630974"},{"database":"DOI","id":"10.1111/j.1365-2958.2007.05799.x"}],"title":"Multiple interactions among the competence proteins of Bacillus subtilis.","publicationDate":"2007","journal":"Mol. Microbiol.","firstPage":"454","lastPage":"464","volume":"65"},"referencePositions":["DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"168","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L15202","properties":[{"key":"ProteinId","value":"AAC36907.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Unassigned_DNA"}]},{"database":"EMBL","id":"D84432","properties":[{"key":"ProteinId","value":"BAA12454.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL009126","properties":[{"key":"ProteinId","value":"CAB14499.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S39865","properties":[{"key":"EntryName","value":"S39865"}]},{"database":"RefSeq","id":"NP_390435.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"RefSeq","id":"WP_009967776.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000964.3"}]},{"database":"AlphaFoldDB","id":"P39695","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P39695","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P39695","properties":[{"key":"Number of interactors","value":"232"}]},{"database":"IntAct","id":"P39695","properties":[{"key":"Interactions","value":"2"}]},{"database":"STRING","id":"224308.BSU25570","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.A.11.1.1","properties":[{"key":"FamilyName","value":"the bacterial competence-related dna transformation transporter (dna-t) family"}]},{"database":"PaxDb","id":"224308-BSU25570","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAB14499","properties":[{"key":"ProteinId","value":"CAB14499"},{"key":"GeneId","value":"BSU_25570"}]},{"database":"GeneID","id":"937839","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bsu:BSU25570","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|224308.179.peg.2780","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG0658","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"eggNOG","id":"COG2333","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"P39695","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9761531at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"P39695","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"BSUB:BSU25570-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001570","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"P39695","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0030420","properties":[{"key":"GoTerm","value":"P:establishment of competence for transformation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd07731","properties":[{"key":"EntryName","value":"ComA-like_MBL-fold"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.60.15.10","properties":[{"key":"EntryName","value":"Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR035681","properties":[{"key":"EntryName","value":"ComA-like_MBL"}]},{"database":"InterPro","id":"IPR025405","properties":[{"key":"EntryName","value":"ComEC-like_N"}]},{"database":"InterPro","id":"IPR004477","properties":[{"key":"EntryName","value":"ComEC_N"}]},{"database":"InterPro","id":"IPR004797","properties":[{"key":"EntryName","value":"Competence_ComEC/Rec2"}]},{"database":"InterPro","id":"IPR052159","properties":[{"key":"EntryName","value":"Competence_DNA_uptake"}]},{"database":"InterPro","id":"IPR001279","properties":[{"key":"EntryName","value":"Metallo-B-lactamas"}]},{"database":"InterPro","id":"IPR036866","properties":[{"key":"EntryName","value":"RibonucZ/Hydroxyglut_hydro"}]},{"database":"NCBIfam","id":"TIGR00360","properties":[{"key":"EntryName","value":"ComEC_N-term"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"TIGR00361","properties":[{"key":"EntryName","value":"ComEC_Rec2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30619","properties":[{"key":"EntryName","value":"DNA INTERNALIZATION/COMPETENCE PROTEIN COMEC/REC2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR30619:SF1","properties":[{"key":"EntryName","value":"RECOMBINATION PROTEIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03772","properties":[{"key":"EntryName","value":"Competence"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13567","properties":[{"key":"EntryName","value":"DUF4131"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00753","properties":[{"key":"EntryName","value":"Lactamase_B"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00849","properties":[{"key":"EntryName","value":"Lactamase_B"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56281","properties":[{"key":"EntryName","value":"Metallo-hydrolase/oxidoreductase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQFKAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHIHWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHVGILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVRSATAICLSYIVLLLFNPYHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGAVAGVLLLSLSASFGRLFFSWFDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGGICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKGIKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPEAPDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKNNRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN","length":776,"molWeight":86701,"crc64":"90F50E1EECEEFB2A","md5":"F8839F002594240A259EC344525E308E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":10,"Sequence conflict":2},"uniParcId":"UPI0000060762"}}