{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P39286","secondaryAccessions":["Q2M6E1"],"uniProtkbId":"RSGA_ECOLI","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-12-15","entryVersion":190,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"Small ribosomal subunit biogenesis GTPase RsgA"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"3.6.1.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15466596"}],"value":"rsgA"},"synonyms":[{"value":"engC"},{"value":"yjeQ"}],"orderedLocusNames":[{"value":"b4161"},{"value":"JW4122"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14973029"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15466596"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15828870"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21102555"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25904134"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18223068"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"27382067"}],"value":"One of at least 4 proteins (Era, RbfA, RimM and RsgA/YjeQ) that assist in the late maturation steps of the functional core of the 30S ribosomal subunit (PubMed:18223068, PubMed:21102555, PubMed:21303937, PubMed:25904134, PubMed:27382067). Binds the 30S subunit contacting the head, platform, and rRNA helix 44, which may assist the last maturation stages (PubMed:21788480, PubMed:21960487). Removes RbfA from mature, but not immature 30S ribosomes in a GTP-dependent manner; 95% removal in the presence of GTP, 90% removal in GMP-PNP and 65% removal in the presence of GDP (PubMed:21102555, PubMed:25904134). Circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover (PubMed:12220175). Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96-fold increase in kcat) ribosomes (PubMed:14973029). Mature 30S ribosomes stimulate intrinsic GTPase more than do immature 30S ribosomes (PubMed:25904134). Ribosome-associated GTPase activity is stimulated by RbfA (PubMed:21102555). The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin (PubMed:15466596) streptomycin and spectinomycin (PubMed:15828870). This inhibition is not due to competition for binding sites on the 30S or 70S ribosome (PubMed:15828870)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"Binds 1 zinc ion per subunit."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.41,"unit":"mM","substrate":"GTP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}]},{"constant":1.0,"unit":"mM","substrate":"dATP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}]},{"constant":1.2,"unit":"mM","substrate":"ATP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}]},{"constant":1.3,"unit":"mM","substrate":"ITP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}]},{"constant":1.6,"unit":"mM","substrate":"dGTP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}]},{"constant":2.4,"unit":"mM","substrate":"CTP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12220175"}],"value":"Measured in the absence of 30S ribosomes, kcat is 8.1 h(-1) for GTP, 5.2 for dATP, 3.5 for ATP, 4.5 for ITP, 7.1 for dGTP, 2.1 for CTP."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14973029"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15466596"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21102555"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21788480"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21960487"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25904134"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27382067"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12220175"}],"value":"Monomer (Probable). All of the protein is associated with ribosomes; the ratio is substoichiometric at 1:200 RsgA/ribosome (PubMed:14973029). Association is tightest with the 30S subunit in the presence of the non-hydrolyzable GTP analog GMP-PNP (PubMed:14973029, PubMed:15466596). 2 cryoelectron microscopy (cryo-EM) structures in complex with 30S ribosomes have been resolved; the protein is determined to bind to different but partially overlapping regions of the 30S ribosomal subunit (PubMed:21788480, PubMed:21960487). One cryo-EM study suggests it contacts ribosomal proteins uS3, uS12 and uS13 as well as 16S rRNA (PubMed:21788480). Another cryo-EM study shows it to bind 16S rRNA, no ribosomal proteins, and to cover the sites of intersubunit bridges B2a, B3 and B7a (PubMed:21960487). Has a significant preference for mature versus immature 30S ribosomes in the presence of GMP-PNP (PubMed:21102555, PubMed:27382067). Another study shows it binds equally well to mature and immature 30S ribosomal subunits in the presence of GMP-PNP (PubMed:25904134)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14973029"}],"value":"Associates with 30S ribosomes (PubMed:14973029)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14973029"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25904134"}],"value":"Has 3 domains; an N-terminal OB-like domain (about residues 1-100), a central, circularly permutated GTPase module (residues 104-270) and the C-terminal zinc-finger domain (residues 280 to 350) (PubMed:25904134). The C-terminal domain has 2 regions; the zinc-binding domain (residues 287-319) is required for binding to 30S ribosomes while the extreme C-terminus (residues 320-350) helps remove RbfA from the mature 30S subunit"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15466596"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21102555"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21303937"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25904134"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27382067"}],"value":"Reduced growth rate, reduced 70S ribosomes, accumulation of 17S rRNA (the precursor of 16S rRNA) (PubMed:15466596, PubMed:21303937, PubMed:25904134, PubMed:27382067). Ribosomal proteins uS2 and bS21 not found in 30S subunits, decreased uS3 and RbfA in 30S subunits, distortion of rRNA helix 44 near the decoding center (PubMed:21303937, PubMed:27382067). The phenotype is partially suppressed by overexpression of a number of genes involved in ribosome function, including infB, era and ksgA (PubMed:15466596). Double rbfA-rsgA deletion mutants have the same phenotype as single mutants (PubMed:21102555). Single rgsA deletion is suppressed by a number of mutants in RbfA but not by wild-type RbfA; in all the RbfA mutants less RbfA is found bound to the 30S ribosome (PubMed:21102555)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01820"}],"value":"Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"12220175"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"14973029"}],"value":"Was initially characterized with a propeptide of 20 residues; this is now thought to be the result of in vitro proteolysis when the protein is overexpressed (PubMed:12220175, PubMed:14973029)"}],"commentType":"CAUTION"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAA97060.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":350,"modifier":"EXACT"}},"description":"Small ribosomal subunit biogenesis GTPase RsgA","featureId":"PRO_0000008149"},{"type":"Domain","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":273,"modifier":"EXACT"}},"description":"CP-type 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