{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"P39081","secondaryAccessions":["D6VSL0","Q04932"],"uniProtkbId":"PCF11_YEAST","entryAudit":{"firstPublicDate":"1995-02-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1998-07-15","entryVersion":194,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Protein PCF11"}},"alternativeNames":[{"fullName":{"value":"protein 1 of CF I"}}]},"genes":[{"geneName":{"value":"PCF11"},"orderedLocusNames":[{"value":"YDR228C"}],"orfNames":[{"value":"YD9934.13C"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11344258"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15998810"}],"value":"Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factor NAB4/CFIB and the cleavage and polyadenylation factor (CPF) complex. Independently involved in RNA polymerase II transcript termination. Binds RNA. Seems to bridge RNA polymerase II and the native transcript and may be involved in dismantling the RNA polymerase II elongation complex"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11149954"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12727883"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15565157"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9032237"}],"value":"Component of the CFIA complex, which is composed of RNA14, RNA15, PCF11 and CLP1. Interacts with RNA14, RNA15 and RTT103. Interacts directly with the phosphorylated CTD domain of RPB1/RNA polymerase II"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"P39081","intActId":"EBI-12980"},"interactantTwo":{"uniProtKBAccession":"Q08685","geneName":"CLP1","intActId":"EBI-29732"},"numberOfExperiments":14,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P39081","intActId":"EBI-12980"},"interactantTwo":{"uniProtKBAccession":"P25298","geneName":"RNA14","intActId":"EBI-15632"},"numberOfExperiments":12,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P39081","intActId":"EBI-12980"},"interactantTwo":{"uniProtKBAccession":"P25299","geneName":"RNA15","intActId":"EBI-15640"},"numberOfExperiments":9,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P39081","intActId":"EBI-12980"},"interactantTwo":{"uniProtKBAccession":"P40073","geneName":"SHO1","intActId":"EBI-18140"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"P39081","intActId":"EBI-12980"},"interactantTwo":{"uniProtKBAccession":"Q12159","geneName":"YRA1","intActId":"EBI-29516"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 2800 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":626,"modifier":"EXACT"}},"description":"Protein PCF11","featureId":"PRO_0000058247"},{"type":"Domain","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"CID","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00724"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"Interaction with RBP1 CTD (CID)"},{"type":"Region","location":{"start":{"value":263,"modifier":"EXACT"},"end":{"value":286,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Mutagenesis","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Loss of interaction with RBP1 CTD.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12727883"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["D"]}},{"type":"Mutagenesis","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"Loss of interaction with RBP1 CTD.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12727883"}],"alternativeSequence":{"originalSequence":"DSI","alternativeSequences":["AAA"]}},{"type":"Sequence conflict","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":288,"modifier":"EXACT"}},"description":"in Ref. 3; AAC33145","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"NSL","alternativeSequences":["ILS"]}},{"type":"Sequence conflict","location":{"start":{"value":515,"modifier":"EXACT"},"end":{"value":515,"modifier":"EXACT"}},"description":"in Ref. 3; AAC33145","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["V"]}},{"type":"Helix","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":16,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":40,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1SZ9"}]},{"type":"Helix","location":{"start":{"value":474,"modifier":"EXACT"},"end":{"value":476,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4C0B"}]},{"type":"Helix","location":{"start":{"value":481,"modifier":"EXACT"},"end":{"value":485,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OI4"}]},{"type":"Helix","location":{"start":{"value":486,"modifier":"EXACT"},"end":{"value":490,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OI4"}]},{"type":"Helix","location":{"start":{"value":495,"modifier":"EXACT"},"end":{"value":498,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4OI4"}]},{"type":"Helix","location":{"start":{"value":543,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Beta strand","location":{"start":{"value":550,"modifier":"EXACT"},"end":{"value":553,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Turn","location":{"start":{"value":555,"modifier":"EXACT"},"end":{"value":557,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5M9Z"}]},{"type":"Beta strand","location":{"start":{"value":561,"modifier":"EXACT"},"end":{"value":564,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Turn","location":{"start":{"value":565,"modifier":"EXACT"},"end":{"value":568,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Beta strand","location":{"start":{"value":569,"modifier":"EXACT"},"end":{"value":576,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Turn","location":{"start":{"value":577,"modifier":"EXACT"},"end":{"value":580,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Beta strand","location":{"start":{"value":581,"modifier":"EXACT"},"end":{"value":585,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Beta strand","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":590,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Beta strand","location":{"start":{"value":593,"modifier":"EXACT"},"end":{"value":596,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]},{"type":"Helix","location":{"start":{"value":597,"modifier":"EXACT"},"end":{"value":603,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2NAX"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"id":"KW-0806","category":"Biological process","name":"Transcription termination"}],"references":[{"referenceNumber":1,"citation":{"id":"9169867","citationType":"journal article","authors":["Jacq C.","Alt-Moerbe J.","Andre B.","Arnold W.","Bahr A.","Ballesta J.P.G.","Bargues M.","Baron L.","Becker A.","Biteau N.","Bloecker H.","Blugeon C.","Boskovic J.","Brandt P.","Brueckner M.","Buitrago M.J.","Coster F.","Delaveau T.","del Rey F.","Dujon B.","Eide L.G.","Garcia-Cantalejo J.M.","Goffeau A.","Gomez-Peris A.","Granotier C.","Hanemann V.","Hankeln T.","Hoheisel J.D.","Jaeger W.","Jimenez A.","Jonniaux J.-L.","Kraemer C.","Kuester H.","Laamanen P.","Legros Y.","Louis E.J.","Moeller-Rieker S.","Monnet A.","Moro M.","Mueller-Auer S.","Nussbaumer B.","Paricio N.","Paulin L.","Perea J.","Perez-Alonso M.","Perez-Ortin J.E.","Pohl T.M.","Prydz H.","Purnelle B.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Rieger M.","Salom D.","Saluz H.P.","Saiz J.E.","Saren A.-M.","Schaefer M.","Scharfe M.","Schmidt E.R.","Schneider C.","Scholler P.","Schwarz S.","Soler-Mira A.","Urrestarazu L.A.","Verhasselt P.","Vissers S.","Voet M.","Volckaert G.","Wagner G.","Wambutt R.","Wedler E.","Wedler H.","Woelfl S.","Harris D.E.","Bowman S.","Brown D.","Churcher C.M.","Connor R.","Dedman K.","Gentles S.","Hamlin N.","Hunt S.","Jones L.","McDonald S.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","Rajandream M.A.","Richards C.","Shore L.","Walsh S.V.","Barrell B.G.","Dietrich F.S.","Mulligan J.T.","Allen E.","Araujo R.","Aviles E.","Berno A.","Carpenter J.","Chen E.","Cherry J.M.","Chung E.","Duncan M.","Hunicke-Smith S.","Hyman R.W.","Komp C.","Lashkari D.","Lew H.","Lin D.","Mosedale D.","Nakahara K.","Namath A.","Oefner P.","Oh C.","Petel F.X.","Roberts D.","Schramm S.","Schroeder M.","Shogren T.","Shroff N.","Winant A.","Yelton M.A.","Botstein D.","Davis R.W.","Johnston M.","Andrews S.","Brinkman R.","Cooper J.","Ding H.","Du Z.","Favello A.","Fulton L.","Gattung S.","Greco T.","Hallsworth K.","Hawkins J.","Hillier L.W.","Jier M.","Johnson D.","Johnston L.","Kirsten J.","Kucaba T.","Langston Y.","Latreille P.","Le T.","Mardis E.","Menezes S.","Miller N.","Nhan M.","Pauley A.","Peluso D.","Rifkin L.","Riles L.","Taich A.","Trevaskis E.","Vignati D.","Wilcox L.","Wohldman P.","Vaudin M.","Wilson R.","Waterston R.","Albermann K.","Hani J.","Heumann K.","Kleine K.","Mewes H.-W.","Zollner A.","Zaccaria P."],"citationCrossReferences":[{"database":"PubMed","id":"9169867"},{"database":"DOI","id":"10.1038/387s075"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.","publicationDate":"1997","journal":"Nature","firstPage":"75","lastPage":"78","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"7885847","citationType":"journal article","authors":["Davies C.J.","Hutchison C.A. III"],"citationCrossReferences":[{"database":"PubMed","id":"7885847"},{"database":"DOI","id":"10.1093/nar/23.3.507"}],"title":"Insertion site specificity of the transposon Tn3.","publicationDate":"1995","journal":"Nucleic Acids Res.","firstPage":"507","lastPage":"514","volume":"23"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-626"]},{"referenceNumber":4,"citation":{"id":"9032237","citationType":"journal article","authors":["Amrani N.","Minet M.","Wyers F.","Dufour M.-E.","Aggerbeck L.P.","Lacroute F."],"citationCrossReferences":[{"database":"PubMed","id":"9032237"},{"database":"DOI","id":"10.1128/mcb.17.3.1102"}],"title":"PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I.","publicationDate":"1997","journal":"Mol. Cell. Biol.","firstPage":"1102","lastPage":"1109","volume":"17"},"referencePositions":["IDENTIFICATION IN THE CFIA COMPLEX","INTERACTION WITH RNA14 AND RNA15"]},{"referenceNumber":5,"citation":{"id":"11344258","citationType":"journal article","authors":["Gross S.","Moore C."],"citationCrossReferences":[{"database":"PubMed","id":"11344258"},{"database":"DOI","id":"10.1073/pnas.101046598"}],"title":"Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I.","publicationDate":"2001","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"6080","lastPage":"6085","volume":"98"},"referencePositions":["FUNCTION OF THE CFIA COMPLEX"]},{"referenceNumber":6,"citation":{"id":"11149954","citationType":"journal article","authors":["Barilla D.","Lee B.A.","Proudfoot N.J."],"citationCrossReferences":[{"database":"PubMed","id":"11149954"},{"database":"DOI","id":"10.1073/pnas.98.2.445"}],"title":"Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae.","publicationDate":"2001","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"445","lastPage":"450","volume":"98"},"referencePositions":["INTERACTION WITH RBP1"]},{"referenceNumber":7,"citation":{"id":"12727883","citationType":"journal article","authors":["Sadowski M.","Dichtl B.","Huebner W.","Keller W."],"citationCrossReferences":[{"database":"PubMed","id":"12727883"},{"database":"DOI","id":"10.1093/emboj/cdg200"}],"title":"Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination.","publicationDate":"2003","journal":"EMBO J.","firstPage":"2167","lastPage":"2177","volume":"22"},"referencePositions":["INTERACTION WITH RBP1","MUTAGENESIS OF ALA-66 AND 68-ASP--ILE-70"]},{"referenceNumber":8,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":9,"citation":{"id":"15565157","citationType":"journal article","authors":["Kim M.","Krogan N.J.","Vasiljeva L.","Rando O.J.","Nedea E.","Greenblatt J.F.","Buratowski S."],"citationCrossReferences":[{"database":"PubMed","id":"15565157"},{"database":"DOI","id":"10.1038/nature03041"}],"title":"The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II.","publicationDate":"2004","journal":"Nature","firstPage":"517","lastPage":"522","volume":"432"},"referencePositions":["INTERACTION WITH RTT103"]},{"referenceNumber":10,"citation":{"id":"15998810","citationType":"journal article","authors":["Zhang Z.","Fu J.","Gilmour D.S."],"citationCrossReferences":[{"database":"PubMed","id":"15998810"},{"database":"DOI","id":"10.1101/gad.1296305"}],"title":"CTD-dependent dismantling of the RNA polymerase II elongation complex by the pre-mRNA 3'-end processing factor, Pcf11.","publicationDate":"2005","journal":"Genes Dev.","firstPage":"1572","lastPage":"1580","volume":"19"},"referencePositions":["FUNCTION","RNA-BINDING"]},{"referenceNumber":11,"citation":{"id":"15241417","citationType":"journal article","authors":["Meinhart A.","Cramer P."],"citationCrossReferences":[{"database":"PubMed","id":"15241417"},{"database":"DOI","id":"10.1038/nature02679"}],"title":"Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors.","publicationDate":"2004","journal":"Nature","firstPage":"223","lastPage":"226","volume":"430"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-140"]},{"referenceNumber":12,"citation":{"id":"15665873","citationType":"journal article","authors":["Noble C.G.","Hollingworth D.","Martin S.R.","Ennis-Adeniran V.","Smerdon S.J.","Kelly G.","Taylor I.A.","Ramos A."],"citationCrossReferences":[{"database":"PubMed","id":"15665873"},{"database":"DOI","id":"10.1038/nsmb887"}],"title":"Key features of the interaction between Pcf11 CID and RNA polymerase II CTD.","publicationDate":"2005","journal":"Nat. Struct. Mol. Biol.","firstPage":"144","lastPage":"151","volume":"12"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-138"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"Z48612","properties":[{"key":"ProteinId","value":"CAA88508.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"U13239","properties":[{"key":"ProteinId","value":"AAC33145.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006938","properties":[{"key":"ProteinId","value":"DAA12070.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S59435","properties":[{"key":"EntryName","value":"S59435"}]},{"database":"RefSeq","id":"NP_010514.3","properties":[{"key":"NucleotideSequenceId","value":"NM_001180536.3"}]},{"database":"PDB","id":"1SZ9","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"A/B/C=1-140"}]},{"database":"PDB","id":"1SZA","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.20 A"},{"key":"Chains","value":"A/B/C=1-140"}]},{"database":"PDB","id":"2BF0","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"X=1-138"}]},{"database":"PDB","id":"2NAX","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=538-608"}]},{"database":"PDB","id":"2NPI","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.95 A"},{"key":"Chains","value":"C/D=454-563"}]},{"database":"PDB","id":"4C0B","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.77 A"},{"key":"Chains","value":"C/D=454-563"}]},{"database":"PDB","id":"4C0H","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"C/D=454-563"}]},{"database":"PDB","id":"4OI4","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"B/D/U=454-563"}]},{"database":"PDB","id":"5M9Z","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=530-626"}]},{"database":"PDBsum","id":"1SZ9","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"1SZA","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2BF0","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2NAX","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2NPI","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4C0B","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4C0H","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4OI4","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5M9Z","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"BMRB","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"32280","properties":[{"key":"Interactions","value":"391"}]},{"database":"ComplexPortal","id":"CPX-1895","properties":[{"key":"EntryName","value":"mRNA cleavage factor complex CFIA"}]},{"database":"ComplexPortal","id":"CPX-1896","properties":[{"key":"EntryName","value":"mRNA cleavage factor complex CFI"}]},{"database":"DIP","id":"DIP-944N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"P39081","properties":[{"key":"Number of interactors","value":"590"}]},{"database":"IntAct","id":"P39081","properties":[{"key":"Interactions","value":"88"}]},{"database":"MINT","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YDR228C","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"P39081","properties":[{"key":"glycosylation","value":"1 site"}]},{"database":"iPTMnet","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YDR228C","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YDR228C_mRNA","properties":[{"key":"ProteinId","value":"YDR228C"},{"key":"GeneId","value":"YDR228C"}]},{"database":"GeneID","id":"851814","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YDR228C","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000002636","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000002636","properties":[{"key":"GeneName","value":"PCF11"}]},{"database":"VEuPathDB","id":"FungiDB:YDR228C","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2071","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_015606_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PHANKNI","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2129491at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-29807-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-SCE-9770562","properties":[{"key":"PathwayName","value":"mRNA Polyadenylation"}]},{"database":"BioGRID-ORCS","id":"851814","properties":[{"key":"hits","value":"1 hit in 10 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"P39081","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:P39081","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome IV"}]},{"database":"RNAct","id":"P39081","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22932476"}]},{"database":"GO","id":"GO:0005849","properties":[{"key":"GoTerm","value":"C:mRNA cleavage factor complex"},{"key":"GoEvidenceType","value":"IPI:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"11344258"},{"evidenceCode":"ECO:0000353","source":"PubMed","id":"22026644"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22932476"}]},{"database":"GO","id":"GO:0003729","properties":[{"key":"GoTerm","value":"F:mRNA binding"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15998810"}]},{"database":"GO","id":"GO:0000993","properties":[{"key":"GoTerm","value":"F:RNA polymerase II complex binding"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12727883"}]},{"database":"GO","id":"GO:0031124","properties":[{"key":"GoTerm","value":"P:mRNA 3'-end processing"},{"key":"GoEvidenceType","value":"IDA:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22026644"}]},{"database":"GO","id":"GO:0006369","properties":[{"key":"GoTerm","value":"P:termination of RNA polymerase II transcription"},{"key":"GoEvidenceType","value":"IDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12727883"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15998810"}]},{"database":"GO","id":"GO:0030847","properties":[{"key":"GoTerm","value":"P:termination of RNA polymerase II transcription, exosome-dependent"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"12819204"}]},{"database":"GO","id":"GO:0030846","properties":[{"key":"GoTerm","value":"P:termination of RNA polymerase II transcription, poly(A)-coupled"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"12819204"}]},{"database":"CDD","id":"cd16982","properties":[{"key":"EntryName","value":"CID_Pcf11"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.25.40.90:FF:000016","properties":[{"key":"EntryName","value":"mRNA cleavage factor complex component Pcf11"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.90","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR006569","properties":[{"key":"EntryName","value":"CID_dom"}]},{"database":"InterPro","id":"IPR008942","properties":[{"key":"EntryName","value":"ENTH_VHS"}]},{"database":"InterPro","id":"IPR045154","properties":[{"key":"EntryName","value":"PCF11-like"}]},{"database":"InterPro","id":"IPR054127","properties":[{"key":"EntryName","value":"Pcf11_C"}]},{"database":"InterPro","id":"IPR047415","properties":[{"key":"EntryName","value":"Pcf11_CID"}]},{"database":"InterPro","id":"IPR021605","properties":[{"key":"EntryName","value":"Pcf11_Clp1-ID"}]},{"database":"InterPro","id":"IPR054128","properties":[{"key":"EntryName","value":"Pfc11_Rna14/15-ID"}]},{"database":"PANTHER","id":"PTHR15921:SF3","properties":[{"key":"EntryName","value":"PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR15921","properties":[{"key":"EntryName","value":"PRE-MRNA CLEAVAGE COMPLEX II"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04818","properties":[{"key":"EntryName","value":"CID"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21936","properties":[{"key":"EntryName","value":"Pcf11_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11526","properties":[{"key":"EntryName","value":"Pfc11_Clp1_ID"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21940","properties":[{"key":"EntryName","value":"Pfc11_Rna14-15-ID"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00582","properties":[{"key":"EntryName","value":"RPR"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48464","properties":[{"key":"EntryName","value":"ENTH/VHS domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51391","properties":[{"key":"EntryName","value":"CID"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDHDTEVIVKDFNSILEELTFNSRPIITTLTKLAEENISCAQYFVDAIESRIEKCMPKQKLYAFYALDSICKNVGSPYTIYFSRNLFNLYKRTYLLVDNTTRTKLINMFKLWLNPNDTGLPLFEGSALEKIEQFLIKASALHQKNLQAMLPTPTVPLLLRDIDKLTCLTSERLKNQPNDEKLKMKLLVLSQLKQELKREKLTLNALKQVQMQLRQVFSQDQQVLQERMRYHELQQQQQQQQQQQQQQQQQQQQYHETKDMVGSYTQNSNSAIPLFGNNSDTTNQQNSLSSSLFGNISGVESFQEIEKKKSLNKINNLYASLKAEGLIYTPPKESIVTLYKKLNGHSNYSLDSHEKQLMKNLPKIPLLNDILSDCKAYFATVNIDVLNNPSLQLSEQTLLQENPIVQNNLIHLLYRSKPNKCSVCGKRFGNSESEKLLQNEHLDWHFRINTRIKGSQNTANTGISNSNLNTTTTRKNIQSRNWYLSDSQWAAFKDDEITSTKHKNDYTDPHANKNIDKSALNIHADENDEGSVDNTLGSDRSNELEIRGKYVVVPETSQDMAFKCPICKETVTGVYDEESGEWVWKNTIEVNGKYFHSTCYHETSQNSSKSNSGKVGLDDLKKLVTK","length":626,"molWeight":71898,"crc64":"66F91BA9577E82F1","md5":"0E53835DFCC2EB2A0DB2A319EC53992F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":5,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":2,"Mutagenesis":2,"Sequence conflict":2,"Helix":16,"Beta strand":6,"Turn":3},"uniParcId":"UPI0000052DDB"}}